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731.
Anna R. Carta Anil Kachroo Nicoletta Schintu Kui Xu Michael A. Schwarzschild Jadwiga Wardas Micaela Morelli 《Journal of neurochemistry》2009,111(6):1478-1489
Adenosine A2A receptors antagonists produce neuroprotective effects in animal models of Parkinson’s disease (PD). As neuroinflammation is involved in PD pathogenesis, both neuronal and glial A2A receptors might participate to neuroprotection. We employed complementary pharmacologic and genetic approaches to A2A receptor inactivation, in a multiple MPTP mouse model of PD, to investigate the cellular basis of neuroprotection by A2A antagonism. MPTP·HCl (20 mg/kg daily for 4 days) was administered in mice treated with the A2A antagonist SCH58261, or in conditional knockout mice lacking A2A receptors on forebrain neurons (fbnA2AKO mice). MPTP‐induced partial loss of dopamine neurons in substantia nigra pars compacta (SNc) and striatum (Str), associated with increased astroglial and microglial immunoreactivity in these areas. Astroglia was similarly activated 1, 3, and 7 days after MPTP administration, whereas maximal microglial reactivity was detected on day 1, returning to baseline 7 days after MPTP administration. SCH58261 attenuated dopamine cell loss and gliosis in SNc and Str. Selective depletion of A2A receptors in fbnA2AKO mice completely prevented MPTP‐induced dopamine neuron degeneration and gliosis in SNc, and partially counteracted gliosis in Str. Results provide evidence of a primary role played by neuronal A2A receptors in neuroprotective effects of A2A antagonists in a multiple MPTP injections model of PD. With the symptomatic antiparkinsonian potential of several A2A receptor antagonists being pursued in clinical trials, this study adds to the rationale for broader clinical benefit and use of these drugs early in the treatment of PD. 相似文献
732.
Cinzia Murgia Rachele Berria Luigi Minerba Simonetta Sulis Michela Murenu Elaine Portoghese Nicoletta Garau Pierina Zedda Gian Benedetto Melis 《Reproductive biology and endocrinology : RB&E》2008,6(1):26
Background
A very high prevalence (22.3%) of gestational diabetes mellitus (GDM) was recently reported following our study on a large group of Sardinian women. In order to explain such a high prevalence we sought to characterise our obstetric population through the analysis of risk factors and their association with the development of GDM. 相似文献733.
Laura Moro Marina Colombi Nicoletta Zoppi Annalisa Ghinelli Sergio Barlati 《Cell biology international》1994,18(1):29-37
Skin fibroblasts derived from Ehlers-Danlos syndrome (EDS) patients lack an organized extracellular matrix (ECM) of fibronectin (FN) and often show an accumulation of cytoplasmic FN. The treatment of EDS cells of different types (I to VIII) with 10-7 M dexamethasone (dex), as well as cocultivation with control fibroblasts, induced in most cases the assembly of a FN-like ECM. The study of FN mRNA expression by dot-blot hybridization and of FN released into the culture media of EDS cells showed that the correction of the defective FN-ECM of EDS cells by dex treatment is associated in most cases with an increase of FN mRNA synthesis and of secreted FN. 相似文献
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735.
Summary Four techniques for the electrophoretic separation of human haptoglobins have been experimented. The results are compared, and the conclusion drawn that disc-electrophoresis with 5.5% acrylamide (Cyanogum), ammonium persulphate as gelling catalyst, and TRIS-glycine buffer for the electrode compartments, gives the optimal separation of Hp polymers.
This work has been supported in part by grant 115. 1839. 04817 from CNR. 相似文献
Zusammenfassung Vier Techniken für die elektrophoretische Trennung der menschlichen Haptoglobine wurden erprobt. Die Resultate werden verglichen und der Schluß gezogen, daß Disc-Electrophoresis mit 5,5% Acrylamide (Cyanogum), Ammoniumpersulphate als Katalysator und TRIS-Glycine-Puffersystem für die Elektrodenfächer die beste Trennung der Hp-Polymere gibt.
This work has been supported in part by grant 115. 1839. 04817 from CNR. 相似文献
736.
Nicoletta Basilico 《Analytical biochemistry》2009,385(2):371-373
We provide two simple low-cost and low-tech procedures to measure with good precision and accuracy the binding and internalization into human erythrocytes of chloroquine and other aminoquinolines. The methods are based on the high fluorescence of the quinoline ring and are complementary. Method A evaluates residual drugs in the supernatants of treated erythrocytes, whereas method B quantifies the total uptake by whole cells and the fraction bound to the membranes. Drug uptake is dose dependent and related to the number of erythrocytes. These assays could be useful when studying the cell interaction of quinoline-type compounds not available in the radioactive form. 相似文献
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738.
739.
Francesca Zambianchi Stefano Raimondi Piero Pasta Giacomo Carrea Nicoletta Gaggero John M. Woodley 《Journal of Molecular Catalysis .B, Enzymatic》2004,31(4-6):165-171
Both whole cells of recombinant Escherichia coli TOP10, overexpressing cyclohexanone monooxygenase (CHMO) and isolated cyclohexanone monooxygenase, were used to carry out the enantioselective oxidation of 1,3-dithiane (1) to (R)-1,3-dithiane-1-oxide (2). The two biocatalysts were evaluated under various experimental conditions (e.g., shaken flask or bioreactor; non-bound or resin-adsorbed substrate; different substrate concentrations) in terms of volumetric productivity and enantioselectivity. While productivity was similar in the two cases (up to 0.58 g L−1 h−1), the optical purity of the product was much higher with the isolated enzyme (up to 98% e.e.) than with the whole cell biocatalyst (up to 85% e.e.). 相似文献
740.
Julian Buchrieser Jrmy Dufloo Mathieu Hubert Blandine Monel Delphine Planas Maaran Michael Rajah Cyril Planchais Franoise Porrot Florence GuivelBenhassine Sylvie Van der Werf Nicoletta Casartelli Hugo Mouquet Timothe Bruel Olivier Schwartz 《The EMBO journal》2021,40(3)
In the supporting information of the article, the authors noticed that there was an error in Movie EV1. The right panel (SARS‐CoV‐2 + IFITM1) showed the same PI channel data (red) as the middle panel (SARS‐CoV‐2). This mistake occurred during the assembly of the merged movie file and does not change the interpretation of the data. A corrected version of the movie is herewith updated. 相似文献