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61.
Genovesio A Kwon YJ Windisch MP Kim NY Choi SY Kim HC Jung S Mammano F Perrin V Boese AS Casartelli N Schwartz O Nehrbass U Emans N 《Journal of biomolecular screening》2011,16(9):945-958
Recent genome-wide RNAi screens have identified >842 human genes that affect the human immunodeficiency virus (HIV) cycle. The list of genes implicated in infection differs between screens, and there is minimal overlap. A reason for this variance is the interdependence of HIV infection and host cell function, producing a multitude of indirect or pleiotropic cellular effects affecting the viral infection during RNAi screening. To overcome this, the authors devised a 15-dimensional phenotypic profile to define the viral infection block induced by CD4 silencing in HeLa cells. They demonstrate that this phenotypic profile excludes nonspecific, RNAi-based side effects and viral replication defects mediated by silencing of housekeeping genes. To achieve statistical robustness, the authors used automatically annotated RNAi arrays for seven independent genome-wide RNAi screens. This identified 56 host genes, which reliably reproduced CD4-like phenotypes upon HIV infection. The factors include 11 known HIV interactors and 45 factors previously not associated with HIV infection. As proof of concept, the authors confirmed that silencing of PAK1, Ku70, and RNAseH2A impaired HIV replication in Jurkat cells. In summary, multidimensional, visual profiling can identify genes required for HIV infection. 相似文献
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Blinks and saccades cause transient interruptions of visual input. To investigate how such effects influence our perceptual
state, we analyzed the time courses of blink and saccade rates in relation to perceptual switching in the Necker cube. Both
time courses of blink and saccade rates showed peaks at different moments along the switching process. A peak in blinking
rate appeared 1,000 ms prior to the switching responses. Blinks occurring around this peak were associated with subsequent
switching to the preferred interpretation of the Necker cube. Saccade rates showed a peak 150 ms prior to the switching response.
The direction of saccades around this peak was predictive of the perceived orientation of the Necker cube afterwards. Peak
blinks were followed and peak saccades were preceded by transient parietal theta band activity indicating the changing of
the perceptual interpretation. Precisely-timed blinks, therefore, can initiate perceptual switching, and precisely-timed saccades
can facilitate an ongoing change of interpretation. 相似文献
63.
Crisafulli E Beneventi C Bortolotti V Kidonias N Fabbri LM Chetta A Clini EM 《PloS one》2011,6(8):e23770
Background
Measurements of Energy Expenditure (EE) at rest (REE) and during physical activities are increasing in interest in chronic patients. In this study we aimed at evaluating the validity/reliability of the SenseWear®Armband (SWA) device in terms of REE and EE during assisted walking in Chronic Respiratory Failure (CRF) patients receiving long-term oxygen therapy (LTOT).Methodology/Principal Findings
In a two-phase prospective protocol we studied 40 severe patients and 35 age-matched healthy controls. In phase-1 we determined the validity and repeatability of REE measured by SWA (REEa) in comparison with standard calorimetry (REEc). In phase-2 we then assessed EE and Metabolic Equivalents-METs by SWA during the 6-minute walking test while breathing oxygen in both assisted (Aid) or unassisted (No-Aid) modalities. When compared with REEc, REEa was slightly lower in patients (1351±169 vs 1413±194 kcal/day respectively, p<0.05), and less repeatable than in healthy controls (0.14 and 0.43 coefficient respectively). COPD patients with CRF patients reported a significant gain with Aid as compared with No-Aid modality in terms of meters walked, perceived symptoms and EE.Conclusions/Significance
SWA provides a feasible and valid method to assess the energy expenditure in CRF patients on LTOT, and it shows that aided walking results in a substantial energy saving in this population. 相似文献64.
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Novel Adeno‐Associated Viral Vector Delivering the Utrophin Gene Regulator Jazz Counteracts Dystrophic Pathology in mdx Mice
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