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971.
972.
Suresh Kumar Seong Won Choi Yuexi Gu Michal Mudd Nicolas Dupont Shanya Jiang Ryan Peters Farzin Farzam Ashish Jain Keith A Lidke Christopher M Adams Terje Johansen Vojo Deretic 《The EMBO journal》2017,36(1):42-60
Autophagy is a process delivering cytoplasmic components to lysosomes for degradation. Autophagy may, however, play a role in unconventional secretion of leaderless cytosolic proteins. How secretory autophagy diverges from degradative autophagy remains unclear. Here we show that in response to lysosomal damage, the prototypical cytosolic secretory autophagy cargo IL‐1β is recognized by specialized secretory autophagy cargo receptor TRIM16 and that this receptor interacts with the R‐SNARE Sec22b to recruit cargo to the LC3‐II+ sequestration membranes. Cargo secretion is unaffected by downregulation of syntaxin 17, a SNARE promoting autophagosome–lysosome fusion and cargo degradation. Instead, Sec22b in combination with plasma membrane syntaxin 3 and syntaxin 4 as well as SNAP‐23 and SNAP‐29 completes cargo secretion. Thus, secretory autophagy utilizes a specialized cytosolic cargo receptor and a dedicated SNARE system. Other unconventionally secreted cargo, such as ferritin, is secreted via the same pathway. 相似文献
973.
A. Tyagi J. Hermans J. Steppuhn Ch. Jansson F. Vater R. G. Herrmann 《Molecular & general genetics : MGG》1987,207(2-3):288-293
Summary Several cDNA clones encoding the 33 kDa protein associated with the photosynthetic water oxidation activity of spinach were sequenced. A 1208 bp insert of one of the clones encodes the entire 331 amino acid residues of the precursor protein including 84 amino acids (8.5 kDa) of the amino-terminal transit peptide, 49 bp of the 5 and 111 bp of the 3 untranslated segment of the mRNA. The 3 poly(A) tail starts 19 bp downstream from a putative polyadenylation signal, TATAAA. The hydrophilic mature protein consists of 247 amino acid residues corresponding to an Mr of 26.5 kDa, which is 6.5 kDa smaller than the value determined by SDS-polyacrylamide gel electrophoresis (33–34 kDa), and shows a certain degree of conservation with the putative Mn-complexing active sites of bacterial Mn-dependent superoxide dismutases. The anatomy of the unusually long transit sequence is discussed with regard to current concepts of protein import into and protein routein within the organelle. 相似文献
974.
Natural antibody--complement dependent neutralization of bovine herpesvirus 4 by human serum 总被引:1,自引:0,他引:1
Machiels B Gillet L Nascimento Brito SD Drion P Delforge C Nizet Y Gianello P Bona C Costes B Markine-Goriaynoff N Vanderplasschen A 《Microbes and infection / Institut Pasteur》2007,9(14-15):1530-1537
In contrast to most gammaherpesviruses, Bovine herpesvirus 4 (BoHV-4) has a broad range of host species both in vitro and in vivo. Several in vitro studies demonstrated that some human cell lines are sensitive or even permissive to BoHV-4. These observations led to the hypothesis that cross-species transmission of BoHV-4 could lead to human infections. In the present study, we investigate the sensitivity of BoHV-4 to neutralization by na?ve human sera in order to determine if humans exhibit innate anti-viral activities against this virus. Our results demonstrate that human sera from na?ve individuals, in contrast to the sera of na?ve subjects from various animal species, neutralize BoHV-4 efficiently. A series of complementary experiments were performed to unravel the mechanism(s) of this neutralization. The data obtained in this study demonstrates that human serum neutralizes BoHV-4 in a complement dependent manner activated by natural antibodies raised against the Galalpha1-3Galbeta1-4GlcNAc-R epitope expressed by bovine cells. 相似文献
975.
Pia Gruber Filipe Carvalho Marco P. C. Marques Brian O'Sullivan Fabiana Subrizi Dragana Dobrijevic John Ward Helen C. Hailes Pedro Fernandes Roland Wohlgemuth Frank Baganz Nicolas Szita 《Biotechnology and bioengineering》2018,115(3):586-596
976.
977.
978.
J. Ch. Mühlefeld 《Plant Systematics and Evolution》1866,16(5):149-156
Ohne Zusammenfassung 相似文献
979.
Ch. Luerssen 《Plant Systematics and Evolution》1863,13(8):162-161
Ohne Zusammenfassung 相似文献
980.
Pfarrer Ch. Münch 《Plant Systematics and Evolution》1862,12(2):35-37
Ohne Zusammenfassung 相似文献