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91.
Metabolic effects of phenethyl alcohol on mammalian cells 总被引:1,自引:0,他引:1
92.
Mitochondrial hexokinase. Release, rebinding, and location 总被引:30,自引:0,他引:30
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1. Rat tissues have been shown to possess an aminotransferase that is active towards l-tyrosine O-sulphate and dependent on 2-oxoglutarate and pyridoxal phosphate. 2. Kidney, liver and pancreas have the greatest activity and the enzyme is localized mainly in the mitochondrial fraction in the liver and kidney cell. 3. The enzyme was shown to be distinct from l-tyrosine-2-oxoglutarate aminotransferase but its true identity was not established. 4. A procedure for the assay of the enzyme in crude tissue preparations was developed. 相似文献
97.
Rose B. Saperstein 《The Western journal of medicine》1961,94(4):211-213
Long term continuous superficial abrasion with abrasive compounds of various degrees of roughness was used over an 11-year period in over 1,500 patients with acne of all types. The following observations were made:• That when a material with a specific abrasive quality is used for a period, desquamation stops despite continued use of the material, and then use of material of the next higher degree of roughness is indicated until the patient''s skin is blackhead-free and minutely flaky.• When long term continuous superficial graded abrasion is used alone or with other treatment, it permits the young patients to outgrow the disease with little or no scarring.• The routine of abrasive washing seems in a large measure to replace the constant fingering of lesions by the patient.• No untoward effects were observed clinically. There was no hyperpigmentation of Negro or Caucasian skins. 相似文献
98.
Cytoplasmic crystalline inclusions are found in some larval haemocytes of Drosophila melanogaster. Blackening can be experimentally induced in these cells, and previously it was suggested that either the substrate or enzyme for the tyrosine-tyrosinase system leading to melanin production in Drosophila larvae is found in these inclusions in the crystal cells. The present report is an attempt to further localize the enzyme and substrate. Larvae have been fed on food containing α-C14-tyrosine and autoradiographs of the blood cells taken from these larvae subsequently prepared. The C14 activity in the crystal cells is restricted to the crystal inclusions in the cells and is significantly higher than that found in the other type of haemocytes, the plasmatocytes. When samples of the blood cells are incubated in DOPA solution, the extra-crystalline cytoplasm becomes blackened while the crystals themselves remain colorless. These observations are consistent with the hypothesis that the substrate is localized in the crystal inclusions whereas enzyme is found in the surrounding cytoplasm. An in vivo structural isolation would serve to separate enzyme and substrate rather than an inhibition by dehydrogenases as postulated by previous authors. In vitro examination with the phase microscope has shown that the crystal cells rupture easily and the crystals dissolve in the haemolymph. Therefore any treatment which tends to disrupt the structural integrity of the cell will allow the enzyme and substrate to come together. Humoral factors preceding metamorphosis might account for the in vivo release of the enzymatic reaction by initially altering the permeability of the cell. 相似文献
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