全文获取类型
收费全文 | 1493篇 |
免费 | 122篇 |
出版年
2023年 | 11篇 |
2022年 | 16篇 |
2021年 | 39篇 |
2020年 | 23篇 |
2019年 | 37篇 |
2018年 | 29篇 |
2017年 | 28篇 |
2016年 | 46篇 |
2015年 | 77篇 |
2014年 | 85篇 |
2013年 | 99篇 |
2012年 | 141篇 |
2011年 | 144篇 |
2010年 | 84篇 |
2009年 | 66篇 |
2008年 | 112篇 |
2007年 | 93篇 |
2006年 | 93篇 |
2005年 | 62篇 |
2004年 | 60篇 |
2003年 | 69篇 |
2002年 | 57篇 |
2001年 | 26篇 |
2000年 | 9篇 |
1999年 | 10篇 |
1998年 | 14篇 |
1997年 | 10篇 |
1996年 | 11篇 |
1995年 | 6篇 |
1994年 | 2篇 |
1993年 | 11篇 |
1992年 | 6篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1989年 | 3篇 |
1988年 | 6篇 |
1987年 | 3篇 |
1986年 | 2篇 |
1985年 | 5篇 |
1984年 | 4篇 |
1983年 | 1篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1972年 | 1篇 |
排序方式: 共有1615条查询结果,搜索用时 421 毫秒
991.
992.
993.
Van Den Hende S Vervaeren H Saveyn H Maes G Boon N 《Biotechnology and bioengineering》2011,108(3):549-558
Microalgal bacterial floc (MaB‐floc) reactors have been suggested as a more sustainable secondary wastewater treatment. We investigated whether MaB‐flocs could be used as tertiary treatment. Tertiary influent has a high inorganic/organic carbon ratio, depending on the efficiency of the secondary treatment. In this study, the effect of this inorganic/organic carbon ratio on the MaB‐flocs performance was determined, using three sequencing batch photobioreactors. The MaB‐flocs were fed with synthetic wastewater containing 84, 42, and 0 mg L−1 C‐KHCO3 supplemented with 0, 42, 84 mg L−1 C‐sucrose, respectively, representing inorganic versus organic carbon. Bicarbonate significantly decreased the autotrophic index of the MaB‐flocs and resulted in poorly settling flocs. Moreover, sole bicarbonate addition led to a high pH of 9.5 and significant lower nitrogen removal efficiencies. Sucrose without bicarbonate resulted in good settling MaB‐flocs, high nitrogen removal efficiencies and neutral pH levels. Despite the lower chlorophyll a content of the biomass and the lower in situ oxygen concentration, 92–96% of the soluble COD‐sucrose was removed. This study shows that the inorganic/organic carbon ratio of the wastewater is of major importance and that organic carbon is requisite to guarantee a good performance of the MaB‐flocs for wastewater treatment. Biotechnol. Bioeng. 2011; 108:549–558. © 2010 Wiley Periodicals, Inc. 相似文献
994.
Chong DY Hansen UN van der Venne R Verdonschot N Amis AA 《Journal of biomechanics》2011,44(5):948-954
Periprosthetic bone resorption after tibial prosthesis implantation remains a concern for long-term fixation performance. The fixation techniques may inherently aggravate the "stress-shielding" effect of the implant, leading to weakened bone foundation. In this study, two cemented tibial fixation cases (fully cemented and hybrid cementing with cement applied under the tibial tray leaving the stem uncemented) and three cementless cases relying on bony ingrowth (no, partial and fully ingrown) were modelled using the finite element method with a strain-adaptive remodelling theory incorporated to predict the change in the bone apparent density after prosthesis implantation. When the models were loaded with physiological knee joint loads, the predicted patterns of bone resorption correlated well with reported densitometry results. The modelling results showed that the firm anchorage fixation formed between the prosthesis and the bone for the fully cemented and fully ingrown cases greatly increased the amount of proximal bone resorption. Bone resorption in tibial fixations with a less secure anchorage (hybrid cementing, partial and no ingrowth) occurred at almost half the rate of the changes around the fixations with a firm anchorage. The results suggested that the hybrid cementing fixation or the cementless fixation with partial bony ingrowth (into the porous-coated prosthesis surface) is preferred for preserving proximal tibial bone stock, which should help to maintain post-operative fixation stability. Specifically, the hybrid cementing fixation induced the least amount of bone resorption. 相似文献
995.
996.
The relationships of 16 leaf traits and their plasticity with the dependence of tree species on gaps for regeneration (gap association index; GAI) were examined in a Neotropical rainforest. Young saplings of 24 species with varying GAI were grown under a closed canopy, in a medium-sized and in a large gap, thus capturing the full range of plasticity with respect to canopy openness. Structural, biomechanical, chemical and photosynthetic traits were measured. At the chloroplast level, the chlorophyll a/b ratio and plasticity in this variable were not related to the GAI. However, plasticity in total carotenoids per unit chlorophyll was larger in shade-tolerant species. At the leaf level, leaf mass per unit area (LMA) decreased with the GAI under the closed canopy and in the medium gap, but did not significantly decrease with the GAI in the large gap. This was a reflection of the larger plasticity in LMA and leaf thickness of gap-dependent species. The well-known opposite trends in LMA for adaptation and acclimation to high irradiance in evergreen tropical trees were thus not invariably found. Although leaf strength was dependent on LMA and thickness, plasticity in this trait was not related to the GAI. Photosynthetic capacity expressed on each basis increased with the GAI, but the large plasticity in these traits was not clearly related to the GAI. Although gap-dependent species tended to have a greater plasticity overall, as evident from a principle component analysis, leaf traits of gap-dependent species are thus not invariably more phenotypically plastic. 相似文献
997.
Madhav Prakash Thakur Peter B. Reich Nicholas A. Fisichelli Artur Stefanski Simone Cesarz Tomasz Dobies Roy L. Rich Sarah E. Hobbie Nico Eisenhauer 《Oecologia》2014,175(2):713-723
Global climate warming is one of the key forces driving plant community shifts, such as range shifts of temperate species into boreal forests. As plant community shifts are slow to observe, ecotones, boundaries between two ecosystems, are target areas for providing early evidence of ecological responses to warming. The role of soil fauna is poorly explored in ecotones, although their positive and negative effects on plant species can influence plant community structure. We studied nematode communities in response to experimental warming (ambient, +1.7, +3.4 °C) in soils of closed and open canopy forest in the temperate-boreal ecotone of Minnesota, USA and calculated various established nematode indices. We estimated species-specific coverage of understory herbaceous and shrub plant species from the same experimental plots and tested if changes in the nematode community are associated with plant cover and composition. Individual nematode trophic groups did not differ among warming treatments, but the ratio between microbial-feeding and plant-feeding nematodes increased significantly and consistently with warming in both closed and open canopy areas and at both experimental field sites. The increase in this ratio was positively correlated with total cover of understory plant species, perhaps due to increased predation pressure on soil microorganisms causing higher nutrient availability for plants. Multivariate analyses revealed that temperature treatment, canopy conditions and nematode density consistently shaped understory plant communities across experimental sites. Our findings suggest that warming-induced changes in nematode community structure are associated with shifts in plant community composition and productivity in the temperate-boreal forest ecotones. 相似文献
998.
Ashok K. Rout Marie-Paule Strub Grzegorz Piszczek Nico Tjandra 《The Journal of biological chemistry》2014,289(51):35111-35123
Chaperone-mediated autophagy (CMA) is a highly regulated cellular process that mediates the degradation of a selective subset of cytosolic proteins in lysosomes. Increasing CMA activity is one way for a cell to respond to stress, and it leads to enhanced turnover of non-critical cytosolic proteins into sources of energy or clearance of unwanted or damaged proteins from the cytosol. The lysosome-associated membrane protein type 2a (LAMP-2A) together with a complex of chaperones and co-chaperones are key regulators of CMA. LAMP-2A is a transmembrane protein component for protein translocation to the lysosome. Here we present a study of the structure and dynamics of the transmembrane domain of human LAMP-2A in n-dodecylphosphocholine micelles by nuclear magnetic resonance (NMR). We showed that LAMP-2A exists as a homotrimer in which the membrane-spanning helices wrap around each other to form a parallel coiled coil conformation, whereas its cytosolic tail is flexible and exposed to the cytosol. This cytosolic tail of LAMP-2A interacts with chaperone Hsc70 and a CMA substrate RNase A with comparable affinity but not with Hsp40 and RNase S peptide. Because the substrates and the chaperone complex can bind at the same time, thus creating a bimodal interaction, we propose that substrate recognition by chaperones and targeting to the lysosomal membrane by LAMP-2A are coupled. This can increase substrate affinity and specificity as well as prevent substrate aggregation, assist in the unfolding of the substrate, and promote the formation of the higher order complex of LAMP-2A required for translocation. 相似文献
999.
Nico Salmaso Fabio Buzzi Leonardo Cerasino Letizia Garibaldi Barbara Leoni Giuseppe Morabito Michela Rogora Marco Simona 《Hydrobiologia》2014,731(1):31-48
Winter air and spring surface water temperatures, spring epilimnetic phosphorus, and hypolimnetic oxygen in the deep lakes south of the Alps (Garda, Iseo, Como, Lugano, and Maggiore) showed a high degree of temporal coherence. The common temporal patterns were originating from the effects caused by winter climate, and from corresponding synchronisms in the interannual variations in the extent of the spring water renewal and replenishment of nutrients. In turn, the sequence of linked causal events was triggered by two atmospheric modes of variability relevant for the Mediterranean region, i.e. the East Atlantic pattern (EA) and the Eastern Mediterranean Pattern (EMP). In contrast, there were no significant relationships of air and water temperatures with the North Atlantic Oscillation. In oligotrophic lakes, which were characterised by weak vertical nutrient gradients, the spring replenishment of P was negligible and difficult to detect (Maggiore), or detectable but of minor importance (Garda and Como) compared to that measured in more enriched lakes (Iseo and Lugano). The applicability of EA and EMP in the study of the impact of climate on aquatic ecosystems will require to be tested by expanding the number, typology, and geographical location of water bodies in the Alpine and Mediterranean regions. 相似文献
1000.
Joeri Coppens Bjorge Decostere Stijn Van Hulle Ingmar Nopens Siegfried E. Vlaeminck Leen De Gelder Nico Boon 《Applied microbiology and biotechnology》2014,98(19):8377-8387
Within sustainable resource management, the recovery of nitrogen and phosphorus nutrients from waste streams is becoming increasingly important. Although the use of microalgae has been described extensively in environmental biotechnology, the potential of nitrate-accumulating microalgae for nutrient recovery has not been investigated yet. The ability of these marine microorganisms to concentrate environmental nitrate within their biomass is remarkable. The aim of this study was to investigate the application potential of nitrate-accumulating diatoms for nutrient recovery from marine wastewaters. The intracellular nitrate storage capacity was quantified for six marine diatom strains in synthetic wastewater. Amphora coffeaeformis and Phaeodactylum tricornutum stored the highest amount of nitrate with respectively 3.15 and 2.10 g N L?1 of cell volume, which accounted for 17.3 and 4.6 %, respectively, of the total nitrogen content. The growth and nitrate and phosphate uptake of both diatoms were further analyzed and based on these features P. tricornutum showed the highest potential for nutrient recovery. A mathematical model was developed which included intracellular nitrate storage and the kinetic parameters were derived for P. tricornutum. Furthermore, a simulation study was performed to compare the performance of a proposed microalgal nutrient recovery unit with a conventional denitrification system for marine wastewater treatment. Overall, this study demonstrates the potential application of P. tricornutum for saline wastewater treatment with concurrent nitrogen and phosphorus recycling. 相似文献