Induction of ethylene biosynthesis in tobacco leaf discs by cell wall disesting enzymes

Cellulysin induces ethylene production in tobacco leaf discs by initiating the formation of 1-aminocyclopropane-1-carboxylic acid. Induction occurred within 30 to 60 min of incubation and was inhibited by aminoethoxyvinylglycine, and the antiproteases, PMSF and soybean trypsin inhibitor. Cycloheximide (CHI) at 2.8 μg/ml and chloramphenicol (CAP) at 100 μg/ml did not inhibit this induction although incorporation of the label from (3,4-¹⁴C)methionine into the acid-insoluble fraction was inhibited by 57%. At 14 μg/ml CHI, and CAP, ethylene production was inhibited by 25% while protein synthesis was inhibited by 75%. We suggest that either the low amounts of protein synthesis that appear to be insensitive to CHI is sufficient to induce ethylene biosynthesis or that Cellulysin activates a preexisting but inactive form of ACC synthase to promote ethylene biosynthesis. Also, induction of ethylene production by microbial enzymes that digests plant cell walls may be an initial protective response of plants that serves to combat microbial infection.     

The Lived Experience of Companion-animal Loss: A Systematic Review of Qualitative Studies

The aim of this systematic review was to evaluate qualitative studies of the lived experience of companion-animal loss and grief. Six electronic databases (PsycINFO, CINAHL Plus, Ovid MEDLINE, ProQuest, Scopus, and Web of Science) were searched for English language, peer-reviewed articles from 1970 to July 2015. Only primary empirical studies using a qualitative method with textual data describing a direct ongoing relationship with, and subsequent loss of, a companion animal were included. A narrative synthesis was conducted on 11 eligible studies using inductive open coding techniques. Analysis revealed that pets were often labeled as family, and strong emotional connections between animals and humans were reported in some studies, whereas in other studies findings were inconsistent. Loss experience was predominantly with prototypical animals (cats, dogs); two studies involved other animals (horse, fish). Grief was described in five studies, with participants’ experience ranging from low to overwhelming. Prolonged grief was associated with self-disenfranchisement, whereas subjective healing was associated with remembrance, in which the animal remained as a memory in a “new” normal. Clinical implications are discussed.     

A pilot randomised controlled trial of negative pressure wound therapy to treat grade III/IV pressure ulcers [ISRCTN69032034

ABSTRACT: BACKGROUND: Negative pressure wound therapy (NPWT) is widely promoted as a treatment for full thickness wounds, however there is a lack of high-quality research evidence regarding its clinical and cost effectiveness. A trial of NPWT for the treatment grade III/IV pressure ulcers would be worthwhile but premature without assessing whether such a trial is feasible. The aim of this pilot randomised controlled trial was to assess the feasibility of conducting a future full trial of NPWT for the treatment of grade III and IV pressure ulcers and to pilot all aspects of the trial. METHODS: This was a two centre (acute and community), pilot randomised controlled trial. Eligible participants were randomised to receive either NPWT or Standard Care (SC) (spun hydrocolloid, alginate or foam dressings). The primary outcome measure was time to healing of the reference pressure ulcer. Secondary outcome measures included recruitment rates, frequency of treatment visits, resources used and duration of follow-up. RESULTS: 312 patients were screened for eligibility into this trial over a 12-month recruitment period and 12/312 participants (3.8%) were randomised; six to NPWT and six to SC. Only one reference pressure ulcer healed (NPWT group) during follow up (time to healing 79 days). The mean number of treatment visits per week was 3.1 (NPWT) and 5.7 (SC). 6/6 TNP and 1/6 SC participants withdrew from their allocated trial treatment. The mean duration of follow-up was 3.8 (NPWT) and 5.0 (SC) months. CONCLUSIONS: This pilot trial yielded vital information for the planning of any future full study including a projected recruitment rate, required duration of follow up and extent of research nurse support required. Data were also used to inform cost-effectiveness and value of information analyses which were conducted alongside the pilot trial.     

Adaptive Radiations in the Context of Macroevolutionary Theory: A Paleontological Perspective

Adaptive radiations are often invoked anytime clades show significant bursts of diversification, but it is important to not simply assume that any radiating clade constitutes an adaptive radiation. In addition, several highly relevant macroevolutionary concepts including the Turnover Pulse Hypothesis, the Effect Hypothesis, exaptation, and species selection, have not been considered in the adaptive radiations literature. Here, these concepts are integrated into the theory of evolutionary radiations in general, and adaptive radiations in particular, and different types of evolutionary radiations are identified, including geographic radiations. Special emphasis is placed on considering the role that abiotic as opposed to biotic factors may play in motivating diversification during evolutionary radiations. Further, recent paleontological data suggesting that rather than organismal adaptation it may be principally abiotic factors, such as climate change and a taxon??s presence in a geographically complex region, that cause clades to diversify will be described. The fossil record, the source of the initial hallmark examples of adaptive radiation, now appears to show little concrete support for this phenomenon.