全文获取类型
收费全文 | 1570篇 |
免费 | 180篇 |
出版年
2021年 | 25篇 |
2020年 | 13篇 |
2019年 | 17篇 |
2018年 | 20篇 |
2017年 | 14篇 |
2016年 | 25篇 |
2015年 | 43篇 |
2014年 | 59篇 |
2013年 | 55篇 |
2012年 | 56篇 |
2011年 | 69篇 |
2010年 | 57篇 |
2009年 | 43篇 |
2008年 | 58篇 |
2007年 | 82篇 |
2006年 | 64篇 |
2005年 | 50篇 |
2004年 | 59篇 |
2003年 | 63篇 |
2002年 | 56篇 |
2001年 | 59篇 |
2000年 | 41篇 |
1999年 | 40篇 |
1998年 | 23篇 |
1997年 | 14篇 |
1996年 | 15篇 |
1995年 | 17篇 |
1993年 | 15篇 |
1992年 | 34篇 |
1991年 | 33篇 |
1990年 | 21篇 |
1989年 | 35篇 |
1988年 | 31篇 |
1987年 | 28篇 |
1986年 | 42篇 |
1985年 | 32篇 |
1984年 | 23篇 |
1983年 | 15篇 |
1981年 | 17篇 |
1980年 | 17篇 |
1979年 | 20篇 |
1978年 | 13篇 |
1977年 | 19篇 |
1976年 | 14篇 |
1975年 | 18篇 |
1974年 | 15篇 |
1973年 | 21篇 |
1971年 | 11篇 |
1968年 | 13篇 |
1966年 | 11篇 |
排序方式: 共有1750条查询结果,搜索用时 15 毫秒
51.
P.D. Franzmann B.M. Patterson T.R. Power P.D. Nichols G.B. Davis 《Journal of applied microbiology》1996,80(6):617-625
The city of Perth contains a number of sites that have been contaminated with hydrocarbons due to leakage from petroleum underground storage tanks. Microbial biomass in groundwater and sediment cores from above and below the water table, and from within and outside a plume of hydrocarbon contamination, was examined using phospholipid fatty acid methyl ester analysis. Microbial numbers, calculated from the phospholipid content, ranged from 0·9 × 106 to 7·8 × 106 ' Escherichia coli equivalent cells' g−1 dry wt of sediment. Over 96% of the microbial biomass was attached to the sediment and the proportion of attached cells did not decrease within the plume of contaminants. The amount of biomass within aquifer samples seemed to be related more to the proximity of the rhizosphere to the shallow aquifer, and other unknown urban inputs, rather than to the effects of the plume of contaminants. Fatty acids common to many bacterial groups dominated within the plume, and as such the analyses gave limited insight into microbial community structure. For site assessment of intrinsic remediation of shallow aquifers in urban areas, estimates of microbial biomass may not provide information that is readily applicable to plume management. 相似文献
52.
H. B. Rogers C. A. Beyrouty T. D. Nichols D. C. Wolf C. M. Reynolds 《Soil & Sediment Contamination》1996,5(2):171-186
A mixture of organic chemicals (MOC) containing equal molar amounts of benzoic acid, hexadecane, 2,2‐dimethyl 4,n‐propyl‐benzene, phenanthrene, pyrene, and either cycloheptane or cis‐decahydronaphthalene (cis‐decalin) was applied to soil at rates of 0 to 8000 mg/kg. In a plant‐screening experiment, growth responses of four legume and five nonlegume species were determined at 10 and 25°C. The MOC applied at 2000 mg/kg reduced the growth of several species without resulting in significant seedling death. At 10°C, the growth of alpine bluegrass (Poa alpina L.) in the 1000 and 2000 mg/kg treatments of soil increased by more than 185%. In a plant growth response experiment, alpine bluegrass and alfalfa (Medicago sativa L.) were grown in soil that had been contaminated at rates of 0 and 2000 mg/kg. At 14 weeks, the shoot and root dry weights of alfalfa were 97% lower in the contaminated soil, while the shoot dry weight, root dry weight, and root length of alpine bluegrass were 135,235 and 268% higher, respectively. Except for pyrene, <23% of the compounds comprising the MOC remained in the soil after 4 weeks and <5% after 14 weeks. The disappearance of the MOC was not significantly influenced by the presence of alfalfa or alpine bluegrass. 相似文献
53.
A spontaneous melanotic ependymoma was observed in the brain of an adult female Goeldi's marmoset (Callimico goeldii). The mass completely occupied the left lateral ventricle, rupturing the fornix and corpus callosum, and compressing the adjacent neuropil. Special histochemical techniques, including melanin bleach, periodic acid-Schiff, Perls iron and phosphotungstic acid hematoxylin, demonstrated the neoplasms to be an ependymoma with a rare melanotic differentiation. 相似文献
54.
PcaK, a high-affinity permease for the aromatic compounds 4-hydroxybenzoate and protocatechuate from Pseudomonas putida. 总被引:1,自引:0,他引:1 下载免费PDF全文
PcaK is a transporter and chemoreceptor protein from Pseudomonas putida that is encoded as part of the beta-ketoadipate pathway regulon for aromatic acid degradation. When expressed in Escherichia coli, PcaK was localized to the membrane and catalyzed the accumulation of two aromatic substrates, 4-hydroxybenzoate and protocatechuate, against a concentration gradient. Benzoate inhibited 4-hydroxybenzoate uptake but was not a substrate for PcaK-catalyzed transport. A P. putida pcaK mutant was defective in its ability to accumulate micromolar amounts of 4-hydroxybenzoate and protocatechuate. The mutant was also impaired in growth on millimolar concentrations of these aromatic acids. In contrast, the pcaK mutant grew at wild-type rates on benzoate. The Vmax for uptake of 4-hydroxybenzoate was at least 25 nmol/min/mg of protein, and the Km was 6 microM. PcaK-mediated transport is energized by the proton motive force. These results show that although aromatic acids in the undissociated (uncharged) form can diffuse across bacterial membranes, high-specificity active transport systems probably also contribute to the ability of bacteria to grow on the micromolar concentrations of these compounds that are typically present in soil. A variety of aromatic molecules, including naturally occurring lignin derivatives and xenobiotics, are metabolized by bacteria and may be substrates for transport proteins. The characterization of PcaK provides a foundation for understanding active transport as a critical step in the metabolism of aromatic carbon sources. 相似文献
55.
benK encodes a hydrophobic permease-like protein involved in benzoate degradation by Acinetobacter sp. strain ADP1. 下载免费PDF全文
The chromosomal benK gene was identified within a supraoperonic gene cluster involved in benzoate degradation by Acinetobacter sp. strain ADP1, and benK was expressed in response to a benzoate metabolite, cis,cis-muconate. The disruption of benK reduced benzoate uptake and impaired the use of benzoate or benzaldehyde as the carbon source. BenK was homologous to several aromatic compound transporters. 相似文献
56.
KATP channels were reconstituted in COSm6 cells by coexpression of the sulfonylurea receptor SUR1 and the inward rectifier potassium channel Kir6.2. The role of the two nucleotide binding folds of SUR1 in regulation of KATP channel activity by nucleotides and diazoxide was investigated. Mutations in the linker region and the Walker B motif (Walker, J.E., M.J. Saraste, M.J. Runswick, and N.J. Gay. 1982. EMBO [Eur. Mol. Biol. Organ.] J. 1:945–951) of the second nucleotide binding fold, including G1479D, G1479R, G1485D, G1485R, Q1486H, and D1506A, all abolished stimulation by MgADP and diazoxide, with the exception of G1479R, which showed a small stimulatory response to diazoxide. Analogous mutations in the first nucleotide binding fold, including G827D, G827R, and Q834H, were still stimulated by diazoxide and MgADP, but with altered kinetics compared with the wild-type channel. None of the mutations altered the sensitivity of the channel to inhibition by ATP4−. We propose a model in which SUR1 sensitizes the KATP channel to ATP inhibition, and nucleotide hydrolysis at the nucleotide binding folds blocks this effect. MgADP and diazoxide are proposed to stabilize this desensitized state of the channel, and mutations at the nucleotide binding folds alter the response of channels to MgADP and diazoxide by altering nucleotide hydrolysis rates or the coupling of hydrolysis to channel activation. 相似文献
57.
The carbohydrate metabolism of the mushroom and the respiration of the sporophore after harvest at different stages of growth have been studied. Mannitol and trehalose were found to be the major soluble carbohydrates in the sporophore and mycelium respectively. Mannitol and trehalose levels fall during sporophore storage, and feeding experiments with14C-labelled sugars indicate that they are metabolised. The results were discussed in relation to the post-harvest development of the sporophore. 相似文献
58.
Bacteriophage T5 DNA, when isolated from mature phage particles, contains several nicks in one of the two strands. The 5'-terminal nucleotides at the nicks were labeled with polynucleotide kinase and [gamma-32P]ATP, and the 3'-terminal nucleotides were labeled with Escherichia coli DNA polymerase I and [alpha-32P]dGTP. The sequences around the nicks were analyzed by partial nuclease digestion followed by homochromatography fractionation of the resulting oligonucleotides. The nicks had at least the sequence -PuOH pGpCpGpC- in common. In addition, the two 5' external termini had the first seven nucleotides in common. 相似文献
59.
178-Nucleotide sequence surrounding the cos site of bacteriophage lambda DNA. 总被引:7,自引:1,他引:6 下载免费PDF全文
A nucleotide sequence of 61 nucleotides at the left end and 117 nucleotides at the right end of DNA from bacteriophage lambdacI857Sam7 was determined by the Maxam and Gilbert method. A perfect inverted repeat sequence of 10 nucleotides is near the left end, and one of 15 nucleotides is near the right end. DNA from another closely related lambda strain, lambdacI857prm116Sam7, has about 10% divergence in the sequence of the first 110 nucleotides at the right end and has a 17-member perfect inverted repeat sequence. 相似文献
60.
The hallucinogen analog -2-(2,5-dimethoxy-4-methylphenyl)-cyclopropylamine (DMCPA) was resolved into its two optical isomers. Examination of selected behavioral profiles in mice and cats clearly showed that the levorotatory isomer of DMCPA possesses stereoselective activity when compared with the dextro isomer. The results parallel those obtained using the isomers of the known hallucinogen, DOM (STP) in the same animal models. Comparison of the optical rotatory dispersion (ORD) curves for the N-(5-bromosalicylidene) derivatives of DMCPA and -2-phenylcyclopropylamine (tranylcypromine) of known absolute configuration established the configuration of DMCPA to be (-)-1R,2S. This stereoselective activity and proof of absolute configuration lend strong support to a new model of the hallucinogen receptor. The proposed model suggests possible structural similarities between LSD and phenethylamine hallucinogens. 相似文献