全文获取类型
收费全文 | 734篇 |
免费 | 48篇 |
出版年
2023年 | 4篇 |
2022年 | 5篇 |
2021年 | 17篇 |
2020年 | 12篇 |
2019年 | 14篇 |
2018年 | 17篇 |
2017年 | 15篇 |
2016年 | 17篇 |
2015年 | 54篇 |
2014年 | 43篇 |
2013年 | 49篇 |
2012年 | 69篇 |
2011年 | 68篇 |
2010年 | 35篇 |
2009年 | 37篇 |
2008年 | 35篇 |
2007年 | 41篇 |
2006年 | 38篇 |
2005年 | 38篇 |
2004年 | 28篇 |
2003年 | 35篇 |
2002年 | 21篇 |
2001年 | 10篇 |
2000年 | 10篇 |
1999年 | 5篇 |
1998年 | 5篇 |
1997年 | 3篇 |
1996年 | 6篇 |
1995年 | 5篇 |
1994年 | 5篇 |
1993年 | 4篇 |
1992年 | 2篇 |
1991年 | 5篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 7篇 |
1984年 | 3篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1976年 | 1篇 |
1972年 | 1篇 |
1970年 | 1篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1966年 | 2篇 |
排序方式: 共有782条查询结果,搜索用时 203 毫秒
111.
Using antagonistic soil bacteria and their cell‐free filtrates to control the black rot pathogen Xanthomonas campestris pv. campestris
下载免费PDF全文
![点击此处可从《Journal of Phytopathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Rafael S. da Silva Brenda L. Moutinho Deise R. dos Santos I. S. Vasconcelo‐Rodrigues Viviane Talamini Marcelo F. Fernandes Roberta P. M. Fernandes 《Journal of Phytopathology》2018,166(7-8):494-501
Xanthomonas campestris pv. campestris (Xcc) is a phytopathogenic bacteria, and it is the causative agent of black rot in crucifers. Recent studies have shown that Bacillus species have strong biological control on Xanthomonas. One of the mechanisms of this control is secondary metabolites production. A collection of 257 bacteria isolated from a suppressive soil was evaluated for in vitro antagonistic activity against X. campestris, and 92 isolates (44.6%) were able to inhibit its growth. Among the 92 isolates evaluated in the double‐layer technique, 51 (55.43%) inhibited Xcc growth on the inhibition tests with cell‐free filtrates (CFF) in liquid medium. Thirteen of these isolates presented 50% or more growth inhibition, and five isolates presented 100% growth inhibition of Xcc. The CFF of the isolate TCDT‐08, which belongs to the Paenibacillus genus, was used for in vivo tests with kale crops. The artificial inoculation of kale with Xcc‐629IBSBF pretreated with CFF from the isolate TCDT‐08 demonstrated that the bacterium loses the ability of colonizing kale and of causing black rot. A Paenibacillus sp. isolate has strong inhibitory activity against X. campestris pv. campestris, and further studies can result in the use of this isolate to protect kale from Xcc infection. 相似文献
112.
Lidiane Maria da Silva-Carvalho Danilo Giovanni Narciso Pastura Jennifer Vieira Gomes Priscyanne Barreto Siqueira Mariana Borges Rodrigues Viviane Moreira de Lima Bruno Pereira Berto 《Systematic parasitology》2018,95(5):455-463
A species of Isospora Schneider, 1881 (Protozoa: Apicomplexa: Eimeriidae) considered as new to science is described and characterised molecularly from the eastern white-throated spadebill Platyrinchus mystaceus Vieillot in the Parque Nacional do Itatiaia, southeastern Brazil. Isospora lopesi n. sp. has oöcysts that are subspheroidal to ovoidal, 18–24 × 18–22 (20.6 × 19.7) µm, with smooth, bilayered wall, c.1.5 μm thick. Micropyle and oöcyst residuum are absent, but one polar granule is present. Sporocysts are ellipsoidal, 12–16 × 8–11 (14.4 × 8.6) µm. The Stieda body is flattened to half-moon-shaped and sub-Stieda body is rounded. Sporocyst residuum is present, consisting of numerous spherules of different sizes. Sporozoites are vermiform with anterior and posterior refractile bodies and nucleus. Molecular analysis was conducted at the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. This new isolate exhibited similarity greater than 98% with Isospora spp. isolates from spectacled warblers Sylvia conspicillata Temminck, 1820. This is the fourth isosporoid coccidian described from New World tyrannid birds, but is the first to have a complementary molecular characterisation. 相似文献
113.
Activation of Ca2+-activated K+ (maxi-K+) channel by angiotensin II in myocytes of the guinea pig ileum 总被引:1,自引:0,他引:1
Romero Fernando; Silva Bagnolia A.; Nouailhetas Viviane L.A.; Aboulafia Jeannine 《American journal of physiology. Cell physiology》1998,274(4):C983
We investigatedthe regulation of theCa2+-activatedK+(maxi-K+) channel by angiotensinII (ANG II) and its synthetic analog, [Lys2]ANG II, infreshly dispersed intestinal myocytes. We identified amaxi-K+ channel population in theinside-out patch configuration on the basis of its conductance (257 ± 4 pS in symmetrical 150 mM KCl solution), voltage andCa2+ dependence of channelopening, lowNa+-to-K+andCl-to-K+permeability ratios, and blockade by externalCs+ and tetraethylammoniumchloride. ANG II and[Lys2]ANG II caused anindirect, reversible, Ca2+- anddose-dependent activation ofmaxi-K+ channels in cell-attachedexperiments when cells were bathed inhigh-K+ solution. This effect wasreversibly blocked by DUP-753, being that it is mediated by theAT1 receptor.Evidences that activation of themaxi-K+ channel by ANG II requiresa rise in intracellular Ca2+concentration([Ca2+]i)as an intermediate step were the shift of the open probability of thechannel-membrane potential relationship to less positive membranepotentials and the sustained increase in[Ca2+]iin fura 2-loaded myocytes. The preservation of the pharmacomechanical coupling of ANG II in these cells provides a good model for the studyof transmembrane signaling responses to ANG II and analogs in thistissue. 相似文献
114.
The strong influence of collection bias on biodiversity knowledge shortfalls of Brazilian terrestrial biodiversity
下载免费PDF全文
![点击此处可从《Diversity & distributions》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Ubirajara Oliveira Adriano Pereira Paglia Antonio D. Brescovit Claudio J. B. de Carvalho Daniel Paiva Silva Daniella T. Rezende Felipe Sá Fortes Leite João Aguiar Nogueira Batista João Paulo Peixoto Pena Barbosa João Renato Stehmann John S. Ascher Marcelo Ferreira de Vasconcelos Paulo De Marco Jr Peter Löwenberg‐Neto Priscila Guimarães Dias Viviane Gianluppi Ferro Adalberto J. Santos 《Diversity & distributions》2016,22(12):1232-1244
115.
Viviane Zahner Leon Rabinovitch Phylip Suffys Hooman Momen 《Applied microbiology》1999,65(11):5182-5185
In comparison with other entomopathogenic Bacillus species, the genome of Brevibacillus laterosporus is poorly characterized. The aim of this study was to examine genetic variability in B. laterosporus by using a range of typing methodologies. Strains of B. laterosporus were examined for variation in 13 chromosomal genes encoding enzymes by multilocus enzyme electrophoresis. Optimal conditions of pulsed-field gel electrophoresis and randomly amplified polymorphic DNA were established that allowed analysis of the genome of B. laterosporus. None of these techniques allowed the identification of a convenient molecular marker for entomopathogenic strains, although one specific primer amplified only DNA from almost all mosquitocidal strains. 相似文献
116.
WOX14 promotes bioactive gibberellin synthesis and vascular cell differentiation in Arabidopsis
下载免费PDF全文
![点击此处可从《The Plant journal : for cell and molecular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Erwan Denis Nadia Kbiri Viviane Mary Gaëlle Claisse Natalia Conde e Silva Martin Kreis Yves Deveaux 《The Plant journal : for cell and molecular biology》2017,90(3):560-572
Procambial and cambial stem cells provide the initial cells that allow the formation of vascular tissues. WOX4 and WOX14 have been shown to act redundantly to promote procambial cell proliferation and differentiation. Gibberellins (GAs), which have an important role in wood formation, also stimulate cambial cell division. Here we show that the loss of WOX14 function phenocopies some traits of GA‐deficient mutants that can be complemented by exogenous GA application, whereas WOX14 overexpression stimulates the expression of GA3ox anabolism genes and represses GA2ox catabolism genes, promoting the accumulation of bioactive GA. More importantly, our data clearly indicate that WOX14 but not WOX4 promotes vascular cell differentiation and lignification in inflorescence stems of Arabidopsis. 相似文献
117.
Figueiredo Douglas B. Carvalho Eneas Santos Mauricio P. Kraschowetz Stefanie Zanardo Rafaela T. Campani Gilson Silva Gabriel G. Sargo Cíntia R. Horta Antonio Carlos L. de C. Giordano Roberto Miyaji Eliane N. Zangirolami Teresa C. Cabrera-Crespo Joaquin Gonçalves Viviane Maimoni 《Applied microbiology and biotechnology》2017,101(6):2305-2317
Applied Microbiology and Biotechnology - Streptococcus pneumoniae is the main cause of pneumonia, meningitis, and other conditions that kill thousands of children every year worldwide. The... 相似文献
118.
Ritz MS Millar C Miller GD Phillips RA Ryan P Sternkopf V Liebers-Helbig D Peter HU 《Molecular phylogenetics and evolution》2008,49(1):292-303
Whilst we have now a good understanding how past glaciation influenced species at the northern hemisphere, our knowledge of patterns and modes of speciation is far more limited for the southern hemisphere. We provide mtDNA based data on the phylogeography of a circumpolar distributed southern hemisphere seabird group-the southern skua complex (Catharacta spp.). Diversification of southern skuas dates between 210,000 yBP and 150,000 yBP and coincides with a glacial spanning 230,000-140,000 yBP. Skuas most likely first inhabited the Antarctic continent, in the course of global cooling and increasing glaciation spread to the sub-antarctic islands and Tristan da Cunha and finally colonized Patagonia and the Falkland Islands at the glacial maximum. Despite significant differences between taxa most populations still exchange genes with neighboring populations of other taxa and speciation is incomplete. 相似文献
119.
120.