全文获取类型
收费全文 | 93040篇 |
免费 | 9569篇 |
国内免费 | 68篇 |
出版年
2021年 | 933篇 |
2020年 | 581篇 |
2019年 | 732篇 |
2018年 | 963篇 |
2017年 | 902篇 |
2016年 | 1504篇 |
2015年 | 2414篇 |
2014年 | 2809篇 |
2013年 | 3820篇 |
2012年 | 4709篇 |
2011年 | 4734篇 |
2010年 | 3159篇 |
2009年 | 2861篇 |
2008年 | 4103篇 |
2007年 | 4364篇 |
2006年 | 4178篇 |
2005年 | 3966篇 |
2004年 | 3983篇 |
2003年 | 3896篇 |
2002年 | 3672篇 |
2001年 | 2538篇 |
2000年 | 2319篇 |
1999年 | 2205篇 |
1998年 | 1372篇 |
1997年 | 1129篇 |
1996年 | 1086篇 |
1995年 | 1007篇 |
1994年 | 992篇 |
1993年 | 1027篇 |
1992年 | 1655篇 |
1991年 | 1575篇 |
1990年 | 1554篇 |
1989年 | 1512篇 |
1988年 | 1428篇 |
1987年 | 1370篇 |
1986年 | 1226篇 |
1985年 | 1275篇 |
1984年 | 1216篇 |
1983年 | 1076篇 |
1982年 | 1050篇 |
1981年 | 1073篇 |
1980年 | 1026篇 |
1979年 | 1000篇 |
1978年 | 929篇 |
1977年 | 881篇 |
1976年 | 736篇 |
1975年 | 814篇 |
1974年 | 895篇 |
1973年 | 776篇 |
1972年 | 638篇 |
排序方式: 共有10000条查询结果,搜索用时 62 毫秒
31.
Robert Platt 《BMJ (Clinical research ed.)》1953,1(4810):577-580
32.
33.
34.
35.
36.
Cyclic GMP directly regulates a cation conductance in membranes of bovine rods by a cooperative mechanism 总被引:18,自引:0,他引:18
Cyclic GMP causes the release of endogenous Ca2+ from rod outer segments, whose plasma membrane has been made permeable, or from isolated discs. Approximately 11,000 Ca2+ ions are released per disc at saturating concentrations of cyclic GMP. The velocity and the amplitude of the release of Ca2+ are dependent on the concentration of cyclic GMP. The maximal rate of the Ca2+ efflux is approximately 7 X 10(4) Ca2+ ions s-1 rod-1. The Ca2+ release by cyclic GMP is independent of light. The activation of the efflux occurred within a narrow range of the cyclic GMP concentration (30-80 microM) and does not obey a simple Michaelis-Menten scheme. Instead, the kinetic analysis of the Ca2+ efflux suggests that a minimum number of 2 molecules of cyclic GMP activates the ion conductance in a cooperative fashion. The release of Ca2+ by cyclic GMP requires a gradient of Ca2+ ions across the disc membrane. If the endogenous Ca2+ gradient is dissipated by means of the ionophore A23187, the release of Ca2+ by cyclic GMP is abolished. Ca2+ is released by analogues of cyclic GMP which are either modified at the 8-carbon position of the imidazole ring or by the deaza-analogue of cyclic GMP. Congeners of cyclic GMP which are modified at the ribose, phosphodiester, or pyrimidine portion of the molecule are ineffective. The hydrolysis of cyclic GMP by the light-regulated phosphodiesterase of rod outer segments is not a necessary condition for the Ca2+ release because 8-bromo-cyclic GMP, a congener resistant to hydrolysis, is a more powerful activator of the release than cyclic GMP itself. Ca2+ release by cyclic GMP is inhibited by organic and inorganic blockers of Ca2+ channels. The l-stereoisomer of cis-diltiazem blocks the release of Ca2+ at micromolar concentrations, whereas the d-form is much less effective. These results suggest that disc membranes contain a cationic conductance which is permeable to Ca2+ ions and which is regulated through the cooperative binding of at least 2 molecules of cyclic GMP to regulatory sites of the transport protein. By this mechanism, subtle changes in the concentration of cyclic GMP could promote large changes in the flux of Ca2+ ions across the disc membrane. 相似文献
37.
38.
The roles of specific and nonspecific interactions in the regulation of protein kinase C by lipid have been examined. Binding and activity measurements reveal two mechanisms by which protein kinase C interacts with membranes: (1) a specific binding to the activating lipid phosphatidylserine and (2) a nonspecific binding to nonactivating, acidic lipids. The specific interaction with phosphatidylserine is relatively insensitive to ionic strength, surface charge, and the presence of nonactivating lipids. The two second messengers of the kinase, diacylglycerol and Ca2+, increase markedly the affinity of the kinase for phosphatidylserine. In contrast, the nonspecific interaction is sensitive to ionic strength and surface charge, and is unaffected by diacylglycerol. These results suggest that electrostatic interactions promote the binding of protein kinase C to membranes but the cooperative and selective binding of phosphatidylserine is the dominant driving force in a productive protein-lipid interaction. 相似文献
39.
40.
E. Backe G. Lotz U. Tittelbach H. Thurmer E. Gierke N. Kersten A. Bernard G. Wallenstein W. D. Schneider 《Biomarkers》2000,5(2):119-128
The inflammation markers alpha-1-antitrypsin (AAT), Clara cell protein (CC-16), soluble interleukin-2-receptor (IL-R) and the soluble adhesion molecule E-selectin, the intercellular adhesion molecule (ICAM-1) and the vascular adhesion molecule (VCAM-1) were determined in the serum of 195 salt-exposed miners to analyse dose-response relationships between markers and potash dust. Alpha-1-antitrypsin, Clara-cell protein, IL2-R, E-selectin and VCAM-1 were not changed by salt exposure, however the ICAM-1 level in the serum fell slightly as the salt exposure increased. This effect was strongest in the group of smokers, still visible in the group of ex-smokers, no effect was seen in non-smokers. Markers, with the exception of VCAM-1, were influenced by tobacco exposure. Since markers were not elevated in relation to salt dust exposure, the results do not support an inflammatory effect of potash dust on the respiratory system. 相似文献