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61.
Carolyn Owen Matthaios Mathioudakis Anita Gazivoda Peter Gal Nevena Nol Katerina Kalliampakou Angela Figas Anais Bellan Anais Iparaguirre Luis Rubio Ioannis Livieratos 《Journal of Phytopathology》2014,162(11-12):839-843
Sporadic incidences of Citrus tristeza virus (CTV) in western Crete resulting from the introduction of a mild strain (Spanish isolate T385) have been reported previously. Further analysis within this region has identified an emerging second CTV strain with minimal genetic divergence, sharing 99% nucleotide identity with the severe stem‐pitting isolate Taiwan‐Pum/SP/T1. Other severe isolates from the Mediterranean region appear in the same phylogenetic cluster, indicating movement or new introductions and the need for targeted control actions and improved phytosanitary measures in this area. 相似文献
62.
Ksenija Taški-Ajduković Nevena Nagl Dragan Milić Slobodan Katić Miroslav Zorić 《Central European Journal of Biology》2014,9(8):768-776
The aim of investigation was to evaluate genetic variation and relationship among alfalfa populations and their offspring, with minimal cost, by using DNA marker analysis. RAPD analysis was performed on bulked DNA samples of five alfalfa parental populations and their progenies: 20 F1 populations from reciprocal diallel crosses and five S1 populations from self-pollination. Twenty primers generated 217 bands, ranging in size from 300 to 6000 bp, with the average number of bands per primer of 10.85 and polymorphism information content of 0.246. Percentage of polymorphic loci, effective number of alleles, expected heterozygosity and Shannon’s information index were used to estimate genetic variation. Higher diversity was observed in F1 progeny populations, while genetic variation in parental populations and S1 progenies remained similar. The genetic relatedness of alfalfa populations was analysed by UPGMA and Bayesian model-based clustering approach. In both types of analysis selfpollinated progenies were grouped. Furthermore, the hybrid offspring where Zuzana, and RSI 20 were maternal parents were placed in separate groups. The results indicate that use of RAPD markers on bulked DNA samples can be fast and cost-effective way for differentiation of alfalfa parental populations and their offspring, as well as for evaluation of their genetic relationships. 相似文献
63.
Ignatova Milena Manolova Nevena Rashkov Ilia Vassileva Valya Ignatov George 《Plant and Soil》2000,227(1-2):27-34
The efficacy of Fe3+ complexes of polyethers with 8-quinolinol (8QOH) chelating groups attached to the polymer chain at different positions of
the aromatic ring or having different chain length for remedying the iron-deficient maize plants was evaluated. The efficacy
of chelates of polymers having terminal 8QOH residues was compared with that of complexes of ethylenediaminetetraacetic acid,
8QOH, mixtures of commercial polyethers with isopropylamino end-groups and 8QOH or FeCl3.6H2O. It was found that at 30/25 °C (day/night) and photosynthetic photon flux density 1100–1300 μmol m−2 s−1, the chlorotic maize plants recovered for 4 days of iron re-supply. An increase in the fresh and dry weight, leaf area, net
photosynthetic CO2 uptake of maize leaves, leaf pigment composition and chlorophyll fluorescence was more pronounced in the plants supplied
with Fe3+ chelates of polymers bearing 8QOH groups attached at 5-position, compared to the other tested Fe3+complexes. The importance of the stability of Fe3+ complexes, structure of the chelating agent and the necessity of effective ligand exchange between synthetic chelators and
free phytosiderophore in iron uptake by strategy II plants was discussed.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
64.
Dmitry O. Koltun Eric Q. Parkhill Natalya I. Vasilevich Andrei I. Glushkov Timur M. Zilbershtein Alexei V. Ivanov Andrew G. Cole Ian Henderson Nathan A. Zautke Sandra A. Brunn Nevena Mollova Kwan Leung Jeffrey W. Chisholm Jeff Zablocki 《Bioorganic & medicinal chemistry letters》2009,19(7):2048-2052
We identified a series of structurally novel SCD (Δ9 desaturase) inhibitors via high-throughput screening and follow-up SAR studies. Modification of the central bicyclic scaffold has proven key to our potency optimization effort. The most potent analog (8g) had IC50 value of 50 pM in a HEPG2 SCD assay and has been shown to be metabolically stable and selective against Δ5 and Δ6 desaturases. 相似文献
65.
Dragana Šnjegota Milomir Stefanović Nevena Veličković Duško Ćirović Mihajla Djan 《Conservation Genetics》2018,19(3):755-760
The grey wolves of Bosnia and Herzegovina form a subpopulation of the Dinaric-Balkan wolf population and represent one of Europe’s least studied wolf populations. Since the Dinaric-Balkan population is a valuable source of genetic diversity for neighboring populations, comprehensive assessments are warranted. We aimed to determine the genetic variability and structure of the grey wolf population from Bosnia and Herzegovina, as well as estimate levels of gene flow and inbreeding and evaluate genetic signals of a bottleneck. To do this, we analyzed the variability of eighteen microsatellite loci. We found moderately high genetic heterozygosity for wolves from Bosnia and Herzegovina, as described for other Dinaric-Balkan wolf populations. We reveal weak genetic structuring with two genetic clusters identified. Wolves from the eastern part of the region formed a relatively distinct cluster, whereas individuals in the second cluster overlapped quite considerably with admixed individuals. Despite the signal of genetic structure being weak, clustering of individuals from the eastern part of the country extended through all analyses. Thus, this cluster could be considered a separate management unit, perhaps requiring specific conservation attention. 相似文献
66.
Nevena?Miti? Mariana?Stani?i? Jelena?Savi? Tatjana??osi? Nemanja?Stanisavljevi? Jovanka?Milju?-?uki? Marija?Marin Svetlana?Radovi? Slavica?Ninkovi?Email author 《Protoplasma》2018,255(6):1683-1692
Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nata?a seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes. 相似文献
67.
68.
Contemporary genetic structure and postglacial demographic history of the black scorpionfish,Scorpaena porcus,in the Mediterranean and the Black Seas
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E. Boissin D. Micu M. Janczyszyn‐Le Goff V. Neglia L. Bat V. Todorova M. Panayotova C. Kruschel V. Macic N. Milchakova Ç. Keskin A. Anastasopoulou I. Nasto L. Zane S. Planes 《Molecular ecology》2016,25(10):2195-2209
Understanding the distribution of genetic diversity in the light of past demographic events linked with climatic shifts will help to forecast evolutionary trajectories of ecosystems within the current context of climate change. In this study, mitochondrial sequences and microsatellite loci were analysed using traditional population genetic approaches together with Bayesian dating and the more recent approximate Bayesian computation scenario testing. The genetic structure and demographic history of a commercial fish, the black scorpionfish, Scorpaena porcus, was investigated throughout the Mediterranean and Black Seas. The results suggest that the species recently underwent population expansions, in both seas, likely concomitant with the warming period following the Last Glacial Maximum, 20 000 years ago. A weak contemporaneous genetic differentiation was identified between the Black Sea and the Mediterranean Sea. However, the genetic diversity was similar for populations of the two seas, suggesting a high number of colonizers entered the Black Sea during the interglacial period and/or the presence of a refugial population in the Black Sea during the glacial period. Finally, within seas, an east/west genetic differentiation in the Adriatic seems to prevail, whereas the Black Sea does not show any structured spatial genetic pattern of its population. Overall, these results suggest that the Black Sea is not that isolated from the Mediterranean, and both seas revealed similar evolutionary patterns related to climate change and changes in sea level. 相似文献
69.
70.
Nevena D. McKay Brian Robinson Richard Brodie Nancy Rooke-Allen 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1983,762(2):198-204
Human skin fibroblast cultures, seeded at plate and allowed to grow to confluence at approx. plate, utilized a glycolytic mode of metabolism where the ratio of glucose utilized to lactate produced wa 0.62±0.05 (Zielke, R.H., Ozand, P.T., Tyldon, J.I., Sevdalian, D.A. and Cornblath, M. (1976) Proc. Natl. Acad. Sci. U.S.A. 73, 4110–4114) (mean±S.E.). When the glucose in the medium was exhausted, the lactate produced during the highly glycolytic phase was then reutilized. In monolayer cultures that had been washed with phosphate-buffered saline, rates of glucose utilization were measured at 0.25 and 2 mM glucose by monitoring the appearance of 3H2O from [5-3H]glucose. Rate of utilization for each concentration of glucose decreased markedly as the cultures became more confluent. This decrease also correlated with a reduced ability to transport glucose as measured by 2-deoxy-[3H]glucose uptake in washed monolayer cultures. In washed confluent culture of fibroblasts, glucose utilization was markedly decreased by the presence of pyruvate and lactate but not by glutamine. The respiratory inhibitors, rotenone and antimycin, did not increase the rate of glucose utilization except when added in combination with pyruvate. We conclude that cultured skin fibroblasts posses a highly glycolytic mode of metabolism but that this mode can become more oxidative in the presence of sufficient quantities of pyruvate and lactate. 相似文献