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191.
192.
T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from co-integration of separate T-DNAs 总被引:22,自引:4,他引:18
Myriam De Neve Sylvie De Buck Anni Jacobs Marc Van Montagu Ann Depicker 《The Plant journal : for cell and molecular biology》1997,11(1):15-29
Nicotiana protoplasts and Arabidopsis leaf discs or roots were co-cultivated with two Agrobacterium strains each carrying a different T-DNA. Co-transformed plants were selected and the integration of the different T-DNAs was analysed at the genetic and genomic level. Genetic analysis showed that the T-DNAs derived from different bacteria were frequently integrated at the same locus, independent of the plant species or transformation method used. Southern analysis revealed that 12 out of 27 Arabidopsis transformants contained the co-transferred T-DNAs linked to each other in all possible configurations but with a preference for those with at least one right border involved in linkage. Overall, our data support the hypothesis that ligation of separate T-DNAs is a dominant mechanism in formation of the frequently observed repeats of identical T-DNAs. We propose a scheme which could explain the formation of T-DNA repeats and the preferential involvement of right borders in T-DNA linkages. 相似文献
193.
Liesbeth De Neve Juan J. Soler Manuel Soler Tomás Pérez-Contreras 《Journal of avian biology》2004,35(3):237-245
Reproductive success in many avian populations declines throughout the breeding season. Two hypotheses have gained attention to explain such a decline: the "timing" hypothesis proposes that deteriorating food availability causes the decline in reproductive success (causal effect of breeding time), whereas the "quality" hypothesis proposes that individuals of lower phenotypic quality reproduce at the end of the breeding season, causing the correlation between breeding time and breeding success. We tested both of these hypotheses in a monogamous single breeder, the magpie Pica pica , by experimentally inducing some pairs to lay a replacement clutch, after removal of the first clutch. The first clutch was left in the nest of another magpie pair (matched by laying date and clutch size), and incubated and raised by these foster parents. In this way we obtained two clutches from the same magpie pair with full siblings raised in conditions of the first and second reproductive attempts. High quality pairs (with laying dates in the first half of the breeding season) reached similar breeding success in replacement clutches as compared to first clutches of the same female. In addition, experimental pairs reared significantly more offspring of similar quality in their replacement clutches as compared to late-season first clutches, thereby suggesting that late season first clutches were produced by pairs of lower phenotypic quality. Results indicate that high quality pairs trade-off clutch size for larger eggs in replacement clutches, which could help magpie pairs to partly compensate for poorer environmental conditions associated with a delayed breeding attempt. 相似文献
194.
Liesbeth De Neve Juan J. Soler Manuel Soler Tomás Pérez-Contreras Manuel Martín-Vivaldi Juan G. Martínez 《Journal of avian biology》2004,35(3):246-251
Food availability is an important factor affecting breeding success in birds. Food supplementation experiments in birds have in general focused on the effects on reproductive success in terms of female investment (laying date, clutch size, egg size), however, it is also known that the estimation of mate quality based on sexually selected signals influences female reproductive investment. In the particular case of magpies, females use nest size, a post-mating sexually selected signal, to assess male's likelihood to invest in reproduction, and accordingly adjust reproductive investment (clutch size). Then, the possible effects of food supplementation on female reproductive investment could be mediated by other variables related to parental quality, such as nest size in magpies. In the present study, we explore if higher food availability in a magpie territory affected both male sexually selected traits (i.e. nest size) and female reproductive investment (laying date, egg size, clutch size). We performed a food supplementation experiment in which we experimentally increased food availability in several magpie territories, keeping others as controls. In food-supplemented territories, males built significantly larger nests and females significantly increased egg size by 4.1% compared to control females. Results suggest that the continuous provisioning of protein rich food allowed magpie females to increase egg size. However, laying date and clutch size did not differ between control and food-supplemented magpie pairs. Food availability also affected the relationship between female reproductive investment and nest size. In control territories, females decreased their egg size in response to a larger nest, whereas a tendency for the opposite relationship was revealed in food-supplemented territories. We discuss the possibility that magpie females adopt different strategies for reproductive investment according to food availability. 相似文献
195.
196.
Molecular adaptations underlying susceptibility and resistance to social defeat in brain reward regions 总被引:4,自引:0,他引:4
Krishnan V Han MH Graham DL Berton O Renthal W Russo SJ Laplant Q Graham A Lutter M Lagace DC Ghose S Reister R Tannous P Green TA Neve RL Chakravarty S Kumar A Eisch AJ Self DW Lee FS Tamminga CA Cooper DC Gershenfeld HK Nestler EJ 《Cell》2007,131(2):391-404
While stressful life events are an important cause of psychopathology, most individuals exposed to adversity maintain normal psychological functioning. The molecular mechanisms underlying such resilience are poorly understood. Here, we demonstrate that an inbred population of mice subjected to social defeat can be separated into susceptible and unsusceptible subpopulations that differ along several behavioral and physiological domains. By a combination of molecular and electrophysiological techniques, we identify signature adaptations within the mesolimbic dopamine circuit that are uniquely associated with vulnerability or insusceptibility. We show that molecular recapitulations of three prototypical adaptations associated with the unsusceptible phenotype are each sufficient to promote resistant behavior. Our results validate a multidisciplinary approach to examine the neurobiological mechanisms of variations in stress resistance, and illustrate the importance of plasticity within the brain's reward circuits in actively maintaining an emotional homeostasis. 相似文献
197.
The classic neuropathological diagnostic markers for AD are amyloid plaques and neurofibrillary tangles, but their role in the etiology and progression of the disease remains incompletely defined. Research over the last decade has revealed that cell cycle abnormalities also represent a major neuropathological feature of AD. These abnormalities appear very early in the disease process, prior to the appearance of plaques and tangles; and it has been suggested that neuronal cell cycle regulatory failure may be a significant component of the pathogenesis of AD. The amyloid precursor protein (APP) is most commonly known as the source of the beta-amyloid (Abeta) peptides that accumulate in the brains of patients with AD. However, a large body of work supports the idea that APP is also a signaling receptor. Most recently, it has been shown that familial AD (FAD) mutations in APP or simple overexpression of wild type APP cause dysfunction of APP signaling, resulting in initiation of DNA synthesis in neurons and consequent apoptosis. In this article, we review the evidence that APP has the potential to activate aberrant neuronal cell cycle re-entry in AD, and we describe a signal transduction pathway that may mediate this abnormal activation of the cell cycle. 相似文献
198.
Manuel Soler Elena Macías‐Sánchez David Martín‐Gálvez Liesbeth de Neve 《Journal of avian biology》2017,48(11):1406-1413
Parent decisions about food allocation are usually based on simple time‐saving rules that optimize their own fitness; however, they can sometimes vary depending on the prevailing ecological conditions both outside and inside the nest. Parent–offspring interactions also become more complex when parents suffer from brood parasitism, which implies that they care for the parasite's eggs and unrelated young. The great spotted cuckoo Clamator glandarius is a specialist brood parasite that uses the magpie Pica pica as its primary host. Here, by filming food allocation by magpie parents in natural non‐parasitized and experimentally parasitized and non‐parasitized magpie nests, we have found that magpie provisioning behaviour is highly complex including two types of feedings apart from normal ones. First, false feedings, when the parent touched the chick's beak but did not leave any food, occurred more frequently when feeding a cuckoo than when feeding magpie nestlings. Second, two types of what we have called coax feedings: 2a) when magpie parents induce a nestling to beg by waking it up by touching it softly with the beak, and 2b) when parents disregard begging signals (always from brood parasitic great spotted cuckoos) while coaxing one non‐begging nestling (always one of their own) to feed it. We suggest that brood parasitism, involving selfish excessively begging nestlings, could have acted as a selective pressure for both false and coax feedings to evolve, as both imply ignoring nestlings that beg too much. We also discuss that these parental responses could have evolved either by a discrimination without recognition mechanism, or, more probably, by a recognition‐based discrimination mechanism. 相似文献
199.
200.
Tissue Distribution of Human Monoamine Oxidase A and B mRNA 总被引:5,自引:1,他引:5
Joseph Grimsby Nancy C. Lan Rachael Neve Kevin Chen Jean C. Shih 《Journal of neurochemistry》1990,55(4):1166-1169