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91.
Identification and Localization of a Rickettsia sp. in Bemisia tabaci (Homoptera: Aleyrodidae) 总被引:1,自引:0,他引:1
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Yuval Gottlieb Murad Ghanim Elad Chiel Dan Gerling Vitaly Portnoy Shimon Steinberg Galil Tzuri A. Rami Horowitz Eduard Belausov Neta Mozes-Daube Svetlana Kontsedalov Moshe Gershon Shunit Gal Nurit Katzir Einat Zchori-Fein 《Applied microbiology》2006,72(5):3646-3652
Whiteflies (Homoptera: Aleyrodidae) are sap-sucking insects that harbor “Candidatus Portiera aleyrodidarum,” an obligatory symbiotic bacterium which is housed in a special organ called the bacteriome. These insects are also home for a diverse facultative microbial community which may include Hamiltonella, Arsenophonus, Fritchea, Wolbachia, and Cardinium spp. In this study, the bacteria associated with a B biotype of the sweet potato whitefly Bemisia tabaci were characterized using molecular fingerprinting techniques, and a Rickettsia sp. was detected for the first time in this insect family. Rickettsia sp. distribution, transmission and localization were studied using PCR and fluorescence in situ hybridizations (FISH). Rickettsia was found in all 20 Israeli B. tabaci populations screened but not in all individuals within each population. A FISH analysis of B. tabaci eggs, nymphs, and adults revealed a unique concentration of Rickettsia around the gut and follicle cells, as well as a random distribution in the hemolymph. We postulate that the Rickettsia enters the oocyte together with the bacteriocytes, leaves these symbiont-housing cells when the egg is laid, multiplies and spreads throughout the egg during embryogenesis and, subsequently, disperses throughout the body of the hatching nymph, excluding the bacteriomes. Although the role Rickettsia plays in the biology of the whitefly is currently unknown, the vertical transmission on the one hand and the partial within-population infection on the other suggest a phenotype that is advantageous under certain conditions but may be deleterious enough to prevent fixation under others. 相似文献
92.
Atarhouch T Rüber L Gonzalez EG Albert EM Rami M Dakkak A Zardoya R 《Molecular phylogenetics and evolution》2006,39(2):373-383
Fishery assessment models meant to determine sustainability of commercial marine fish failed to predict recent stock collapses due to overexploitation. One flaw of assessment models is that they strongly rely on catch and age-composition statistics, but largely ignore the genetic background of the studied populations. We examined population genetic structure of sardine (Sardina pilchardus) in the centraleastern and northeastern Atlantic Ocean and Mediterranean Sea to aid fishery management of this heavily fished small pelagic species. We found that sardine has a striking mitochondrial control region, and sequenced a fragment of 387 bp of its 5'-end in 261 individuals collected off the coasts of Morocco (Dakhla, Tantan, Safi, Larache, and Nador), Portugal (Quarteira), Spain (Pasajes, Barcelona), and Greece (Kavala). High levels of haplotypic diversity rendered a rather unresolved NJ phylogeny. The recovered tree had no phylogeographic structuring except for the clustering of 13 individuals of Safi. In contrast, individuals grouped together according to the presence or absence of a 13-bp insertion in the sequence. Phi(ST) pairwise comparisons and molecular variance analyses supported genetic differentiation between the population of Pasajes (Bay of Biscay), and those of the Mediterranean Sea and Moroccan coast, with a contact zone around the Strait of Gibraltar. This result confirms the existence of two subspecies, S. pilchardus pilchardus and S. pilchardus sardina that were previously identified based on meristics and morphometry. Mismatch distribution analysis showed that sardine populations are expanding since the Pleistocene. Surprisingly, the population of Safi showed strong and statistically significant levels of genetic differentiation that could be related with isolation and genetic drift. Comparative analysis of the Safi population versus the rest including mismatch distributions, and a Bayesian skyline plot suggest that the Safi population likely underwent an early genetic bottleneck. The genetic singularity of the Safi population could have been responsible for the historical collapse of this sardine stock in the 1970s. 相似文献
93.
HS1-associated protein X1 (HAX-1) is a mitochondrial protein which interacts with a diverse group of molecules such as inflammatory
cytokines; interleukin-1, hematopoietic lineage specific protein-1 and vimentin. It has been reported that HAX-1 may act as
antiapoptotic protein in HeLa- and Jurkat cells after Fas-treatment, irradiation or serum deprivation. This underlines the
evidence that HAX-1 might be involved in both receptor- and mitochondria-mediated apoptosis pathways. However, the role of
HAX-1 in neuronal death induced by status epilepticus in the immature brain has not been reported. In this study, we performed
a status epilepticus in rats and investigated the dynamic changes of HAX-1 expression, HtrA2 distribution and caspase-3 activation
in the hippocampus. Western blot and immunohistochemistry analysis revealed that HAX-1 was expressed at very low levels in
the hippocampus. Status epilepticus in the immature brain significantly induced increased cytosolic accumulation of HAX-1
in a biphasic manner, induced an upregulation of HtrA2 and enhanced caspase-3 activity in the selectively vulnerable hippocampal
CA1-subfield. Taken together, these results suggested that HAX-1 is probably involved in the pathophysiology of cell death
induced by epilepsy. 相似文献
94.
A reference microsatellite kit to assess for genetic diversity of Sorghum bicolor (Poaceae) 总被引:1,自引:0,他引:1
Billot C Rivallan R Sall MN Fonceka D Deu M Glaszmann JC Noyer JL Rami JF Risterucci AM Wincker P Ramu P Hash CT 《American journal of botany》2012,99(6):e245-e250
? Premise of the study: Discrepancies in terms of genotyping data are frequently observed when comparing simple sequence repeat (SSR) data sets across genotyping technologies and laboratories. This technical concern introduces biases that hamper any synthetic studies or comparison of genetic diversity between collections. To prevent this for Sorghum bicolor, we developed a control kit of 48 SSR markers. ? Methods and Results: One hundred seventeen markers were selected along the genome to provide coverage across the length of all 10 sorghum linkage groups. They were tested for polymorphism and reproducibility across two laboratories (Centre de Cooperation Internationale en Recherche Agronomique pour le Developpement [CIRAD], France, and International Crops Research Institute for the Semi-Arid Tropics [ICRISAT], India) using two commonly used genotyping technologies (polyacrylamide gel-based technology with LI-COR sequencing machines and capillary systems with ABI sequencing apparatus) with DNA samples from a diverse set of 48 S. bicolor accessions. ? Conclusions: A kit for diversity analysis (http://sat.cirad.fr/sat/sorghum_SSR_kit/) was developed. It contains information on 48 technically robust sorghum microsatellite markers and 10 DNA controls. It can further be used to calibrate sorghum SSR genotyping data acquired with different technologies and compare those to genetic diversity references. 相似文献
95.
Sundvall M Korhonen A Vaparanta K Anckar J Halkilahti K Salah Z Aqeilan RI Palvimo JJ Sistonen L Elenius K 《The Journal of biological chemistry》2012,287(27):23216-23226
96.
R Zamora N Azhar R Namas MR Metukuri T Clermont C Gladstone RA Namas L Hermus C Megas G Constantine TR Billiar MP Fink Y Vodovotz 《The Journal of biological chemistry》2012,287(37):31003-31014
Extracellular β-nicotinamide adenine dinucleotide (NAD(+)) is anti-inflammatory. We hypothesized that NAD(+) would modulate the anti-inflammatory cytokine Transforming Growth Factor (TGF)-β1. Indeed, NAD(+) led to increases in both active and latent cell-associated TGF-β1 in RAW 264.7 mouse macrophages as well as in primary peritoneal macrophages isolated from both C3H/HeJ (TLR4-mutant) and C3H/HeOuJ (wild-type controls for C3H/HeJ) mice. NAD(+) acts partially via cyclic ADP-ribose (cADPR) and subsequent release of Ca(2+). Treatment of macrophages with the cADPR analog 3-deaza-cADPR or Ca(2+) ionophores recapitulated the effects of NAD(+) on TGF-β1, whereas the cADPR antagonist 8-Br-cADPR, Ca(2+) chelation, and antagonism of L-type Ca(2+) channels suppressed these effects. The time and dose effects of NAD(+) on TGF-β1 were complex and could be modeled both statistically and mathematically. Model-predicted levels of TGF-β1 protein and mRNA were largely confirmed experimentally but also suggested the presence of other mechanisms of regulation of TGF-β1 by NAD(+). Thus, in vitro and in silico evidence points to NAD(+) as a novel modulator of TGF-β1. 相似文献
97.
98.
99.
Hochwender CG Cha DH Czesak ME Fritz RS Smyth RR Kaufman AD Warren B Neuman A 《Oecologia》2012,169(1):49-60
To determine the mechanistic basis of tolerance, we evaluated six candidate traits for tolerance to damage using F2 interspecific hybrids in a willow hybrid system. A distinction was made between reproductive tolerance and biomass tolerance;
reproductive tolerance was designated as a plant’s proportional change in catkin production following damage, while biomass
tolerance referred to a plant’s proportional change in biomass (i.e., regrowth) following damage. F2 hybrids were generated to increase variation and independence among candidate traits. Using three clonally identical individuals,
pre-damage candidate traits for tolerance to damage (root:shoot ratio, total nonstructural carbohydrate, and total available
protein) and post-damage candidate traits (relative root:shoot ratio, phenolic ratio, and specific leaf area ratio) were measured.
The range of variation for these six candidate traits was broad. Biomass was significantly increased two years after 50% shoot
length removal, and catkin production was not significantly reduced when damaged, suggesting that F2 hybrids had great biomass tolerance and reproductive tolerance. Based on multiple regression methods, increased reproductive
tolerance was associated with increased protein storage and decreased relative root:shoot ratio (reduced root allocation after
damage). In addition, a positive relationship between biomass tolerance and condensed tannins was detected, and both traits
were associated with increased reproductive tolerance. These four factors explained 57% of the variance in the reproductive
tolerance of F2 hybrids, but biomass tolerance explained the majority of the variance in reproductive tolerance. Changes in plant architecture
in response to plant damage may be the underlying mechanism that explains biomass tolerance. 相似文献
100.
Christopher E. Heise Jeremy Murray Katherine E. Augustyn Brandon Bravo Preeti Chugha Frederick Cohen Anthony M. Giannetti Paul Gibbons Rami N. Hannoush Brian R. Hearn Priyadarshini Jaishankar Cuong Q. Ly Kinjalkumar Shah Karen Stanger Micah Steffek Yinyan Tang Xianrui Zhao Joseph W. Lewcock Adam R. Renslo John Flygare Michelle R. Arkin 《PloS one》2012,7(12)
Inhibition of caspase-6 is a potential therapeutic strategy for some neurodegenerative diseases, but it has been difficult to develop selective inhibitors against caspases. We report the discovery and characterization of a potent inhibitor of caspase-6 that acts by an uncompetitive binding mode that is an unprecedented mechanism of inhibition against this target class. Biochemical assays demonstrate that, while exquisitely selective for caspase-6 over caspase-3 and -7, the compound’s inhibitory activity is also dependent on the amino acid sequence and P1’ character of the peptide substrate. The crystal structure of the ternary complex of caspase-6, substrate-mimetic and an 11 nM inhibitor reveals the molecular basis of inhibition. The general strategy to develop uncompetitive inhibitors together with the unique mechanism described herein provides a rationale for engineering caspase selectivity. 相似文献