全文获取类型
收费全文 | 258篇 |
免费 | 35篇 |
出版年
2022年 | 3篇 |
2021年 | 3篇 |
2017年 | 3篇 |
2016年 | 5篇 |
2015年 | 6篇 |
2014年 | 15篇 |
2013年 | 8篇 |
2012年 | 14篇 |
2011年 | 11篇 |
2010年 | 7篇 |
2009年 | 7篇 |
2008年 | 7篇 |
2007年 | 13篇 |
2006年 | 9篇 |
2005年 | 12篇 |
2004年 | 6篇 |
2003年 | 11篇 |
2002年 | 9篇 |
2001年 | 5篇 |
2000年 | 8篇 |
1999年 | 10篇 |
1998年 | 3篇 |
1996年 | 3篇 |
1995年 | 4篇 |
1992年 | 10篇 |
1991年 | 5篇 |
1990年 | 14篇 |
1989年 | 4篇 |
1988年 | 5篇 |
1987年 | 3篇 |
1986年 | 7篇 |
1985年 | 4篇 |
1984年 | 3篇 |
1983年 | 7篇 |
1982年 | 4篇 |
1981年 | 2篇 |
1979年 | 4篇 |
1978年 | 9篇 |
1977年 | 4篇 |
1975年 | 2篇 |
1974年 | 3篇 |
1973年 | 2篇 |
1971年 | 1篇 |
1970年 | 3篇 |
1969年 | 1篇 |
1968年 | 2篇 |
1966年 | 1篇 |
1965年 | 3篇 |
1963年 | 1篇 |
1961年 | 1篇 |
排序方式: 共有293条查询结果,搜索用时 15 毫秒
181.
Inhibition, escape, and attenuated growth of severe acute respiratory syndrome coronavirus treated with antisense morpholino oligomers
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Neuman BW Stein DA Kroeker AD Churchill MJ Kim AM Kuhn P Dawson P Moulton HM Bestwick RK Iversen PL Buchmeier MJ 《Journal of virology》2005,79(15):9665-9676
182.
183.
184.
185.
Robinson WH DiGennaro C Hueber W Haab BB Kamachi M Dean EJ Fournel S Fong D Genovese MC de Vegvar HE Skriner K Hirschberg DL Morris RI Muller S Pruijn GJ van Venrooij WJ Smolen JS Brown PO Steinman L Utz PJ 《Nature medicine》2002,8(3):295-301
We constructed miniaturized autoantigen arrays to perform large-scale multiplex characterization of autoantibody responses directed against structurally diverse autoantigens, using submicroliter quantities of clinical samples. Autoantigen microarrays were produced by attaching hundreds of proteins, peptides and other biomolecules to the surface of derivatized glass slides using a robotic arrayer. Arrays were incubated with patient serum, and spectrally resolvable fluorescent labels were used to detect autoantibody binding to specific autoantigens on the array. We describe and characterize arrays containing the major autoantigens in eight distinct human autoimmune diseases, including systemic lupus erythematosus and rheumatoid arthritis. This represents the first report of application of such technology to multiple human disease sera, and will enable validated detection of antibodies recognizing autoantigens including proteins, peptides, enzyme complexes, ribonucleoprotein complexes, DNA and post-translationally modified antigens. Autoantigen microarrays represent a powerful tool to study the specificity and pathogenesis of autoantibody responses, and to identify and define relevant autoantigens in human autoimmune diseases. 相似文献
186.
Bienkowski PR Barthé A Voloch M Neuman RN Ladisch MR 《Biotechnology and bioengineering》1986,28(7):960-964
Ground corn is now used in industry as an adsorbent to remove water from ethanol vapors. It is stable and inexpensive at 10 cents/lb (22 cents/kg). For regeneration it requires less than 2000 Btu/gal of 190 proof ethanol processed. If necessary, it could be readily saccharified and fermented into ethanol after use. This renewable resource has further exciting potential as an inexpensive adsorbent for water removal from other alcohols, including methanol, isopropanol, and t-butanol. Water sorption capacity in a fixed bed, nonisothermal adsorption column appears to be a function of the heat capacity of the non-adsorbed alcohol vapor, relative to the heat capacity of the corn adsorbent. Methanol, ethanol, isopropanol, and t-butanol containing 17.5 mol% water gave 105,151, 284, and 358 g anhydrous product/kg adsorbent, respectively, per adsorption cycle. This adsorbent, having operational temperature ranges between 80 and 100 degrees C, is indicated to be of potential utility in solvent recycle processes using these industrially important alcohols. Observed adsorption characteristics are discussed in terms of the alcohol properties of molecular size, heat capacity, and diffusivity. The adsorption mechanism is hypothesized to include transport of water molecules into the structure of adjacent starch molecules present in small spherical bodies (diameter of several microns) immobilized on the surface of the corn grit particles. 相似文献
187.
The estimation of the amount of evolutionary divergence that has taken
place between two DNA coding sequences depends strongly on the degree of
constraint on amino acid replacements. If amino acid replacements are
relatively unconstrained, the individual nucleotide is the appropriate unit
of analysis and the method of Tajima and Nei can be used. If amino acid
replacements are constrained, however, this method is shown to be
inapplicable. For sequences with strong amino acid constraints, a method is
outlined analogous to the Tajima and Nei method using codons as the unit of
analysis. Only synonymous substitutions are used. Codon usage data can be
employed to estimate the necessary parameters of the calculation, or a
priori models of substitution may be employed. Sequences with significant
but intermediate constraints on amino acid replacements are, in principle,
unanalyzable.
相似文献
188.
Nerve growth factor increases the cyclic GMP level and activates the cyclic GMP phosphodiesterase in PC12 cells 总被引:2,自引:0,他引:2
Nerve growth factor (NGF) rapidly increases the cyclic GMP (cGMP) level about 2-3-fold and enhances the cGMP phosphodiesterase (PDE) activity about 2-fold in rat pheochromocytoma PC12 cells. No changes in the level of cyclic AMP (cAMP) and in the activity of cAMP PDE were found. GTP and a nonhydrolysable analog of GTP, GMP-PCP, at 100 microM, were able to mimic the effect of NGF on the cGMP PDE activity. These results suggest that the cGMP system may be one of the second messengers of NGF action in PC12 cells. 相似文献
189.
190.
Solute exclusion from cellulose in packed columns: experimental investigation and pore volume measurements 总被引:1,自引:0,他引:1
A packed column approach was used in this investigation to determine pore volume and surface area distributions of several celluloses. Specific surface areas for Avicel PH 102, Solka Floc BW 300, and two size fractions of corn cobs that have been pretreated to remove lignin and hemicellulose were measured using this technique. In addition to measuring pore volume and specific surface area, the molecular diameters of several PEGs (polyethylene glycol) were estimated using viscosity measurements. Also, the influence of cellulose particle size, molecular diameter of PEGs, and PEG solution velocity on dispersion and tailing were investigated. Molecular diameter estimates from this investigation were 30%-35% lower than those reported in the literature. This discrepancy is due to earlier investigators using an inappropriate relationship for estimating molecular diameter from viscosity measurements. The precision of the column approach to solute exclusion was higher than that obtained by investigators using a batch approach. Dispersion increased with increasing particle size. Tailing of the elution curve was increased with increasing solute molecular diameter and elution rate. For a cellulase with a molecular diameter of 5.1 nm, estimated specific surface area ranged from 7.2 to 10.5 m(2) g(-1). 相似文献