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571.
Shirley Raveh Peter Neuhaus F. Stephen Dobson 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2015,370(1669)
Parasites play an important role in the evolution of host traits via natural selection, coevolution and sexually selected ornaments used in mate choice. These evolutionary scenarios assume fitness costs for hosts. To test this assumption, we conducted an ectoparasite removal experiment in free-living Columbian ground squirrels (Urocittelus columbianus) in four populations over three years. Adult females were randomly chosen to be either experimentally treated with anti-parasite treatments (spot-on solution and flea powder, N = 61) or a sham treatment (control, N = 44). We expected that experimental females would show better body condition, increased reproductive success and enhanced survival. Contrary to our expectations, body mass was not significantly different between treatments at mating, birth of litter or weaning of young. Further, neither number nor size of young at weaning differed significantly between the two treatments. Survival to the next spring for adult females and juveniles was not significantly different between experimental and control treatments. Finally, annual fitness was not affected by the treatments. We concluded that females and their offspring were able compensate for the presence of ectoparasites, suggesting little or no fitness costs of parasites for females in the different colonies and during the years of our experiments. 相似文献
572.
Changes in the conductance of the neuronal nicotinic acetylcholine receptor channel induced by magnesium 总被引:3,自引:0,他引:3
We have investigated the effect of magnesium on the single-channel conductance of neuronal nicotinic acetylcholine receptors (nAChRs) in nerve growth-factor treated rat pheochromocytoma (PC12) cells. The patch-clamp technique was used to record single-channel currents from cell attached and excised, outside-out patches in the presence of various internal and external Mg2+ concentrations. Mg2+ reduced the single-channel conductance in a concentration-dependent manner with an IC50 of 9.2 mM for external Mg2+ (inward conductance) and 0.69 mM for external Mg2+ (outward conductance). Both estimated and measured conductances for divalent cation-free CsCl solutions were around 60 pS. We also find that divalent cations are not involved in the inward rectification of whole-cell ACh-induced currents in these cells. Our results imply that the amino acids screened by divalent cations sense electric fields only weakly and are presumably outside the lipid bilayer. They also suggest that the density and the number of charges (or both) differ on either side of the ion pore. 相似文献