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11.
Edgar Antonio Rodríguez-Negrete Enrique Alejandro Guevara-Rivera Ángela Paulina Arce-Leal Norma Elena Leyva-López Jesús Méndez-Lozano 《Molecular Plant Pathology》2023,24(10):1300-1311
The nonstructural protein NSm of tomato spotted wilt virus (TSWV) has been identified as the avirulence determinant of the tomato single dominant Sw-5 resistance gene. Although Sw-5 effectiveness has been shown for most TSWV isolates, the emergence of resistance-breaking (RB) isolates has been observed. It is strongly associated with two point mutations (C118Y or T120N) in the NSm viral protein. TSWV-like symptoms were observed in tomato crop cultivars (+Sw-5) in the Baja California peninsula, Mexico, and molecular methods confirmed the presence of TSWV. Sequence analysis of the NSm 118–120 motif and three-dimensional protein modelling exhibited a noncanonical C118F substitution in seven isolates, suggesting that this substitution could emulate the C118Y-related RB phenotype. Furthermore, phylogenetic and molecular analysis of the full-length genome (TSWV-MX) revealed its reassortment-related evolution and confirmed that putative RB-related features are restricted to the NSm protein. Biological and mutational NSm 118 residue assays in tomato (+Sw-5) confirmed the RB nature of TSWV-MX isolate, and the F118 residue plays a critical role in the RB phenotype. The discovery of a novel TSWV-RB Mexican isolate with the presence of C118F substitution highlights a not previously described viral adaptation in the genus Orthotospovirus, and hence, the necessity of further crop monitoring to alert the establishment of novel RB isolates in cultivated tomatoes. 相似文献
12.
Bruno Moises de Matos Marco Augusto Stimamiglio Alejandro Correa Anny Waloski Robert 《World journal of stem cells》2023,15(5):453-465
Extracellular vesicles (EVs) are nanometric particles that enclose cell-derived bioactive molecules in a lipid bilayer and serve as intercellular communication tools. Accordingly, in various biological contexts, EVs are reported to engage in immune modulation, senescence, and cell proliferation and differentiation. Therefore, EVs could be key elements for potential off-the-shelf cell-free therapy. Little has been studied regarding EVs derived from human pluripotent stem cells (hPSC-EVs), even though hPSCs offer good opportunities for induction of tissue regeneration and unlimited proliferative ability. In this review article, we provide an overview of studies using hPSC-EVs, focusing on identifying the conditions in which the cells are cultivated for the isolation of EVs, how they are characterized, and applications already demonstrated. The topics reported in this article highlight the incipient status of the studies in the field and the significance of hPSC-EVs’ prospective applications as PSC-derived cell-free therapy products. 相似文献
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We argue that the Würm III glaciation eradicated possible European populations ofS. torvicornis, and that today, a reconquest of Europe takes place on two fronts.A western wave has reached the Pyrenees, an eastern one now occupies most of eastern and northern Europe. The western route probably started in the Maghreb, the eastern one in the Levant and the Ponto-Caspian. Animals in the west had to move north by crossing, at right angles, a series of east-west oriented river valleys and progressed slowly; animals in the east could move up river valleys extending north-east, and moved quickly. Italy was not occupied, becauseS.torvicornis is a warm stenotherm, and by the time the climate had warmed sufficiently for it to reach the southern shore of the mediterranean (ca 6000 BP), the gap with Italy was probably too large for a crossing. Cold-loving species (of the generaBranchipus, Chirocephalus) conversely, and could freely flow across the Central Mediterranean at low sea-levels (ca 12000 BP), and now occur in Italy (and the rest of Europe) as well as in Northern Africa.A prediction of our hypothesis is that the pioneer populations in Spain and Central Europe should have been isolated longest. Thisis tested and confirmed by their comparative morphology, and two subspecies,S. t. torvicornis andS. t. bucheti are reinstated.A gap across the Nile Valley where onlyS. rubricaudatus seems to occur, deserves further study. 相似文献
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Diversity, abundance or rare species as a target for the conservation of mammalian carnivores: a case study in Southern Spain 总被引:6,自引:2,他引:4
Alejandro Travaini Miguel Delibes Pablo Ferreras Francisco Palomares 《Biodiversity and Conservation》1997,6(4):529-535
Management goals in protected areas and/or communities usually include diversity as one of the most valuable and confident criteria. Nevertheless, the use of diversity and related indices as a means of evaluating successful management practices could produce conflicting results. Here we report a case study in one of the most important European protected areas. After 6 years of intensive conservation management of the Don~ana National Park, the general abundance and numbers of the target single-species conservation plan (the Iberian lynx) increased, although carnivore community diversity and evenness decreased. This was a result of a disproportionate increase of an oportunistic native species, the red fox. We propose the combined use of diversity, richness and evenness indices when monitoring management practices such as those reported here. 相似文献
17.
A review on salinity adaptation of marine molluscsbased on mainly Russian scientific literature ispresented. The existence of two relativelyindependent systems of adaptation to extreme(resistance level) and moderate (tolerance level)changes of environmental salinity was shown. Theresistance of molluscs is based mainly on an impededwater-salt exchange with the external medium due tomantle cavity hermetization. The tolerance ofmolluscs is determined by cellular mechanisms ofadaptation. Reversible changes of protein and RNAsynthesis, alteration of the pattern of multiplemolecular forms of different enzymes, and theregulation of ionic content and cell volume wereshown to be of importance for the above mentionedmechanisms. The efficiency of resistance andtolerance adaptations to salinity changes may varyin different species and in different colourphenotypes of the same species (intrapopulationalpolymorphism). Parasites (trematodes) may suppressthe resistance of the mollusc-host to extremesalinity changes without effecting the host'scapacity for adaptive changes in salinitytolerance. 相似文献
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Summary A large pedigree with a satellited Yq chromosome is described, Q, C, and NOR banding were performed. Family C proband suffers from a Klinefelter syndrome. 相似文献
20.
Patterns of predation in a free-ranging troop of stumptail macaques (Macaca arctoides): Relations to the ecology II 总被引:2,自引:2,他引:0
Data on patterns of systematic and ocassional hunting of birds, mammals, reptiles, mollusks and insects by Stumptail macaques
are reported for a period of ten months. Systematic hunting of water snails, terrestrial spiders, and land worms was conducted
by all age classes, except infants, and both sexes. Of the occasional hunting of birds, large lizards, large frogs, and field
mice, the adult females conducted 70%, the adult males 12%, the two year old females 12%, and the two year old males 6%. The
differences between males and females were statistically significant (.05 confidence level). Females dominated the hunt and
were more interested in meateating than the males. This contrasts strikingly with the data reported for baboons and chimpanzees
in which the males dominate the hunt.
Of all the prey hunted ocassionally, 76% was shared. The differences between shared and not sared prey were statistically
significant (.05 confidence level). All age classes, including infants, participated in meat-sharing. Three types of meat-sharing
are described: mother-offspring, hunter-close-friend, and piece-dropping. The prey was shared with genetic relatives, and
with close and sistant friends in this order. Pearson’s correlation coeficients between rank of hunter and number of hunts
and between rank of hunter and number of individuals with whom the prey was shared yielded +.866 and +.890 respectively. Meat-sharing
seems to be similar to that observed for baboons but some differences exist between baboons and chimpanzees on the one hand
and Stumptails on the other. Dominance relations in our Stumptails seem to act as the context determining the direction and
the type of sharing.
An increase in hunting activity during the study period is suggested to be the result of the prey’s migratory and breeding
patterns, of environmental changes, and of the high activity scores and physiological states of the adult-females in the troop.
Although not hunted, reactions to snakes, iguanas, scorpions, and gulls are also described.
This work was supported by grants from Behavioral Sciences Foundation and by NSF Grant No. GB-42235. 相似文献