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Identification and quantification of subsets of mononuclear inflammatory cells in melanocytic and other human tumors 总被引:1,自引:0,他引:1
M S Nestor A J Cochran 《Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society》1987,1(1):22-27
We used monoclonal antibodies and an indirect immunoperoxidase technique to identify mononuclear inflammatory cells associated with human tumors. The absolute number of the different types of inflammatory cells was assessed by using a point-counting technique. We studied tissues from six primary cutaneous melanomas, six metastatic melanomas, eight melanocytic nevi, 14 breast cancers, seven examples of fibrocystic disease of the breast, 11 lung cancers, and six colon cancers. Virtually all tumors were associated with substantial numbers of T lymphocytes (Leu3a-positive T helper-inducer cells predominating) and macrophages. Primary melanomas contained significantly more T lymphocytes (P less than .002), macrophages (P less than .005), and Langerhans/dendritic cells (P less than .002) than nevi or normal skin and had a higher proportion of T cells than metastatic melanomas (P less than .01). Breast cancers contained more T lymphocytes and macrophages than occur with fibrocystic disease (P less than .0001 and P less than .002, respectively) and more B lymphocytes. Cancers of the lung and colon contained moderate numbers of T lymphocytes and macrophages; however, colon cancers contained a higher proportion of B cells. Leu7-positive NK/K cells were noted in small numbers in all tumors examined. 相似文献
355.
Myostatin maps to the interval containing the bovine mh locus 总被引:1,自引:0,他引:1
Timothy P.L. Smith Nestor L. Lopez-Corrales Steven M. Kappes Tad S. Sonstegard 《Mammalian genome》1997,8(10):742-744
Myostatin (GDF-8) is a member of the transforming growth factor-β superfamily and plays a role in muscle growth and development.
Mice having targeted disruption of this gene display marked increases in muscle mass, a phenotype similar to the muscular
hypertrophy (mh) in several cattle breeds. Physical mapping data developed from YAC clones indicate the bovine myostatin gene lies close
to the centromere of bovine Chromosome (Chr) 2 (BTA2) at 2q11, indistinguishable from the cytogenetic location of the mh locus. In addition, a polymorphism in the second intron of the gene was used to show that myostatin maps within the interval
previously shown to contain mh. These data suggest myostatin may be the gene causing muscular hypertrophy in cattle.
Received: 19 June 1997 / Accepted: 2 July 1997 相似文献