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11.
Atrazine, simazine, and cyanazine are widely used preemergence and postemergence triazine herbicides that have made their way into the potable water supply of many agricultural communities. Although there are several contradictory genotoxicity studies in the literature, our previous in vitro studies with human lymphocytes showed that atrazine, simazine, and cyanazine did not induce sister chromatid exchanges (SCEs) or chromosome aberrations (CAs) up to the limits of solubility in aqueous medium using 0.5% dimethyl sulfoxide. To expand upon these results and to ensure that our in vitro findings could be replicated in an in vivo system, mice were treated with each triazine by two intraperitoneal injections, 24h apart. The animals were sacrificed and the bone marrow removed for micronucleus (MN) analysis, 24h after the last injection. Two to four independent trials were performed for MN analysis in polychromatic erythrocytes, and in some trials the spleen was removed, cultured, and analyzed for SCEs and CAs. None of the triazines investigated induced MN in the bone marrow, even at doses that caused significant bone marrow suppression and/or death. These results indicate that atrazine, simazine, and cyanazine are not genotoxic as measured by the bone marrow MN assay in mice following high dose exposures. 相似文献
12.
M. A. Pszczolkowski J. F. Brunner M. D. Doerr J. J. Brown 《Journal of Applied Entomology》2004,128(7):474-477
Abstract: Monosodium glutamate, a taste enhancer widely used in food industry, was tested in the laboratory to determine its phagostimulatory effects on larvae of Choristoneura rosaceana (Harris). Larvae fed apple leaves treated with 50–700 μ g/l monosodium glutamate increased leaf tissue consumption by approximately 40%. The stimulatory effect of monosodium glutamate (at 675 μ g/l concentration) was maintained throughout 10 days of continuous exposure. Adding 675 μ g/l monosodium glutamate to commercial formulation of Bacillus thuringiensis ssp. kurstaki, (DiPel® 2X DF), lowered LC50 from 450 to 150 μ g/l (P < 0.05, Lethal Ratio Significance Test), indicating good potential for monosodium glutamate to enhance B. thuringiensis -based formulations. 相似文献
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Jean Yves Le Reste Patrice Nabbe Charles Rivet Charilaos Lygidakis Christa Doerr Slawomir Czachowski Heidrun Lingner Stella Argyriadou Djurdjica Lazic Radost Assenova Melida Hasaganic Miquel Angel Munoz Hans Thulesius Bernard Le Floch Jeremy Derriennic Agnieska Sowinska Harm Van Marwijk Claire Lietard Paul Van Royen 《PloS one》2015,10(1)
Background
Multimorbidity, according to the World Health Organization, exists when there are two or more chronic conditions in one patient. This definition seems inaccurate for the holistic approach to Family Medicine (FM) and long-term care. To avoid this pitfall the European General Practitioners Research Network (EGPRN) designed a comprehensive definition of multimorbidity using a systematic literature review.Objective
To translate that English definition into European languages and to validate the semantic, conceptual and cultural homogeneity of the translations for further research.Method
Forward translation of the EGPRN’s definition of multimorbidity followed by a Delphi consensus procedure assessment, a backward translation and a cultural check with all teams to ensure the homogeneity of the translations in their national context. Consensus was defined as 70% of the scores being higher than 6. Delphi rounds were repeated in each country until a consensus was reachedResults
229 European medical expert FPs participated in the study. Ten consensual translations of the EGPRN comprehensive definition of multimorbidity were achieved.Conclusion
A comprehensive definition of multimorbidity is now available in English and ten European languages for further collaborative research in FM and long-term care. 相似文献14.
15.
Doerr A 《Nature methods》2006,3(6):424
Researchers demonstrate that in addition to protein ligation, folding and functional assays can be performed on proteins while they are still attached to a water-compatible solid support. 相似文献
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Doerr A 《Nature methods》2006,3(5):338
Silica nano test tubes used as delivery vehicles can be 'corked' with nanoparticles using chemical self-assembly. 相似文献
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Doerr A 《Nature methods》2006,3(5):340
With a new method for optimizing the amino acid isotopic labeling pattern, stereo-array isotope labeling (SAIL), structures of large proteins (up to 50 kDa) can now be solved with ease with nuclear magnetic resonance (NMR) spectroscopy. 相似文献
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Doerr A 《Nature methods》2006,3(11):874
An engineered yeast strain is capable of carrying out the full range of human protein glycosylation reactions. 相似文献