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591.
Neri Azar Orna Liarzi Maor Zavitan Mohamed Samara Ahmed Nasser David Ezra 《Microbial biotechnology》2023,16(8):1629-1638
The worldwide demand for reduced and restricted use of pesticides in agriculture due to serious environmental effects, health risks and the development of pathogen resistance calls for the discovery of new bioactive compounds. In the medical field, antibiotic-resistant microorganisms have become a major threat to man, increasing mortality. Endophytes are endosymbiotic microorganisms that inhabit plant tissues without causing any visible damage to their host. Many endophytes secrete secondary metabolites with biological activity against a broad range of pathogens, making them potential candidates for novel drugs and alternative pesticides of natural origin. We isolated endophytes from wild plants in Israel, focusing on endophytes that secrete secondary metabolites with biological activity. We isolated 302 different endophytes from 30 different wild plants; 70 of them exhibited biological activity against phytopathogens. One biologically active fungal endophyte from the genus Penicillium, isolated from a squill (Urginea maritima) leaf, was further examined. Chloroform-based extraction of its growth medium was similarly active against phytopathogens. High-performance liquid chromatography separation followed by gas chromatography/mass spectrometry analysis revealed a single compound—mycophenolic acid—as the main contributor to the biological activity of the organic extract. 相似文献
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Alberto M. Martell Luca M. Neri Loris Zamai Renat Bareggi Lucia Manzoli Lucio Cocco 《The Histochemical journal》1994,26(2):179-188
Summary Isolated nuclei and nuclear matrices, prepared from mouse erythroleukaemia cells, were reacted with the sulphhydryl-specific dye 6-iodoacetamidofluorescein. To determine whether in vitro formation of disulphide bonds might play a role in the nuclear matrix stabilization triggered by exposure of isolated nuclei to the physiological temperature of 37°C, a variety of techniques were employed to assess the state of cysteinyl residues after such an incubation. Both flow cytometry and confocal microscopy quantitative analysis did not reveal major differences in the fluorescence intensity of nuclei incubated at 37°C in comparison with those maintained at 0°C. Confocal scanning laser microscopy revealed that 6-iodoacetamidofluorescein labelled a fibrogranular network in isolated nuclei. The fluorescent pattern of the network was not affected by a 37°C exposure of nuclei. However, such a network was not detectable in isolated nuclear matrices, thus suggesting a possible protein re-arrangement during matrix preparation. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of fluorescent-labelled nuclear proteins showed no difference between heat-exposed and control samples. We conclude that oxidation of cysteinyl residues is not a major factor leading to the stabilization of nuclei incubated at 37°C. 相似文献
594.
Christopher D. Wiley Rishi Sharma Sonnet S. Davis Jose Alberto Lopez-Dominguez Kylie P. Mitchell Samantha Wiley Fatouma Alimirah Dong Eun Kim Therese Payne Andrew Rosko Eliezer Aimontche Sharvari M. Deshpande Francesco Neri Chisaka Kuehnemann Marco Demaria Arvind Ramanathan Judith Campisi 《Cell metabolism》2021,33(6):1124-1136.e5
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Prof.Dr. Franco Russo Dr. Claudio Neri Dr. Adelaide Mastandrea Dr. Alberto Baracca 《Facies》1997,36(1):25-36
Summary The sedimentological features and the microbiofacies of the Cassian platforms (Late Ladinian-Carnian) of the Dolomites can
be studied only on the basis of the socalled “Cipit boulders”, that are platform-derived olistoliths and clasts fed to the
basin and escaped to the extensive dolomitization affecting the buildups.
Our paper deals with the Cipit boulders occurring in the Punta Grohmann section (Wengen and S. Cassiano formations, Late Ladinian,
Archelaus and Regoledanus Zones). The dominant microfacies are represented by boundstone, consisting of nearly 60% of micritic
limestone occurring both as peloidal or aphanitic micrite, mostly organized into stromatolitic laminites of thrombolites.
The skeletal organism (Tubiphytes, skeletal cyanobacteria, sphinctozoan sponges, etc.) represent only a minor component of the rock (usually less than 10%).
Early cements are widespread and consist both of fan-shaped calcite (replacing former aragonite), bladed isopachous magnesian
calcite and radial-fibrous calcite (neomorphic after Mg-calcite). The carbonate platforms from which the olistoliths derive
were made up mainly of carbonate mud that underwent early lithification, as witnessed by the considerable amount of early
cements: therefore they may be regarded to as mudmounds, and more precisely as microbial mud-mounds, due to the clearly accretionary,
organic-controlled nature of most micrites. The micrites, subdivided into auto- and allomicrite on the basis of micromorphological
and fabric characteristics, have been tested for epifluorescence. The results confirm the organic control on the deposition
of automicrite, also in the cases in which a microbial influence is not obvious (i.e. aphanitic micrite without internal organization). 相似文献
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