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111.
Yeast mannans inhibit binding and phagocytosis of zymosan by mouse peritoneal macrophages 总被引:29,自引:2,他引:27 下载免费PDF全文
We have examined the effects of various mannans, glycoproteins, oligosaccharides, monosaccharides, and sugar phosphates on the binding and phagocytosis of yeast cell walls (zymosan) by mouse peritoneal macrophages. A phosphonomannan (PO(4):mannose ratio = 1:8:6) from kloeckera brevis was the most potent inhibitor tested; it inhibited binding and phagocytosis by 50 percent at concentrations of approximately 3-5 μg/ml and 10 μg/ml, respectively. Removal of the phosphate from this mannan by mild acid and alkaline phosphatase treatment did not appreciably reduce its capacity to inhibit zymosan phagocytosis. The mannan from saccharomyces cerevisiae mutant LB301 inhibits phagocytosis by 50 percent at 0.3 mg/ml, and a neutral exocellular glucomannan from pichia pinus inhibited phagocytosis by 50 percent at 1 mg/ml. Cell wall mannans from wild type S. cervisiae X2180, its mnn2 mutant which contains mannan with predominantly 1(arrow)6- linked mannose residues, yeast exocellular mannans and O-phosphonomannans were less efficient inhibitors requiring concentrations of 1-5 mg/ml to achieve 50 percent reduction in phagocytosis. Horseradish peroxidase, which contains high-mannose type oligosaccharides, was also inhibitory. Mannan is a specific inhibitor of zymosan binding and phagocytosis. The binding and ingestion of zymosan but not of IgG- or complement-coated erythrocytes can be obliterated by plating macrophages on substrates coated with poly-L-lysin (PLL)-mannan. Zymosan uptake was completely abolished by trypsin treatment of the macrophages and reduced by 50-60 percent in the presence of 10 mM EGTA. Pretreatment of the macrophages with chloroquine inhibited zymosan binding and ingestion. These results support the proposal that the macrophage mannose/N-acetylglucosamine receptor (P. Stahl, J.S. Rodman, M.J. Miller, and P.H. Schlesinger, 1978, Proc. Natl. Acad. Sci. U.S.A. 75:1399-1403, mediates the phagocytosis of zymosan particles. 相似文献
112.
Photosynthesis in Tall Fescue : IV. Carbon Assimilation Pattern in two Genotypes of Tall Fescue Differing in Net Photosynthesis Rates 总被引:3,自引:2,他引:1 下载免费PDF全文
We previously reported that the net photosynthetic rate of a decaploid genotype (I-16-2) of tall fescue (Festuca arundinacea Schreb.) was 32 to 41 versus 22 milligrams CO2 per square decimeter per hour in a hexaploid genotype (V6-802) (Randall, Nelson, Asay Plant Physiol 59: 38-41). The high rate was later correlated with increases in total ribulose 1,5-bisphosphate carboxylase protein (17%) and activity (27%) (Joseph, Randall, Nelson Plant Physiol 68: 894-898). This report characterizes photosynthesis with respect to light saturation and early products of photosynthesis in an attempt to identify regulatory metabolic site(s) in these two genotypes. Analysis of the early products of photosynthesis indicated that both genotypes fixed CO2 via the Calvin-Benson cycle with phosphoglyceric acid as the initial primary product. Both genotypes had similar 14C-labeled intermediates. Sucrose was the primary sink of 14CO2 assimilation. After 10 min of 14CO2 assimilation with attached leaves, sucrose accounted for 89% (decaploid) and 81% (hexaploid) of the total 14C incorporated. In 10 min, this amounted to 1.3 (decaploid) and 0.8 (hexaploid) μmol [14C]sucrose formed g fresh weight−1 and reflected the observed differences in photosynthetic rates. There was limited labeling of starch (1%) and fructan (1%). Results of total nonstructural carbohydrates and Pi analysis also demonstrated sucrose was the predominant carbohydrate in fescue leaves. Quantitative differences in sucrose and Pi between the two genotypes may reflect changes in partitioning and this possibility is discussed. 相似文献
113.
A M Bersten Q F Ahkong T Hallinan S J Nelson J A Lucy 《Biochimica et biophysica acta》1983,762(3):429-436
The effects of competitive inhibitors of transglutaminase on the formation of myotubes by the fusion of myoblasts in vitro has been investigated. Myotube formation was inhibited when myoblasts from 11-day-old chick embryos were cultured in vitro in the presence of 10 mM histamine or 0.2 mM dansyl cadaverine. The inhibitions observed were reversed when the treated cells were subsequently cultured in normal medium. Glycine methyl ester also inhibited myotube formation but sarcosine methyl ester, which is not a competitive inhibitor of transglutaminase, had little if any inhibitory action. The formation of myotubes was not inhibited by cultivation in normal medium adjusted to pH 8.0-8.1, indicating that the observed effects of histamine and of dansyl cadaverine were not mediated by a lysosomotropic effect. Inhibition of myotube formation in the presence of histamine was accompanied by the production of abnormal multinucleated cells, indicating that myoblast fusion occurred in the treated cultures but that the fused cells failed to elongate into normal myotubes. Transglutaminase activity has been found in cell-free lysates of embryonic chick myoblasts and it is concluded that a transglutaminase enzyme, activated by an increase in the concentration of intracellular Ca2+, plays an important role in stabilising the cytoskeletal network of developing myotubes. 相似文献
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116.
An invertase inactivator in maize endosperm and factors affecting inactivation 总被引:15,自引:10,他引:5 下载免费PDF全文
A protein present in the developing endosperm of maize (Zea mays L.) causes a loss of invertase activity under certain conditions of incubation. This protein, designated an inactivator, inactivates invertase I of maize even in the presence of other proteins. No inactivation of invertase II of maize or yeast invertase has been observed. The inactivator and invertase I are found only in the endosperm. The quantity of inactivator increases in the normal endosperm during development while invertase I activity decreases. However, the altered levels of invertase I activity in several endosperm mutant lines do not result from different quantities of inactivator. The inactivator can decrease invertase I activity during a preincubation period before addition of sucrose; inactivation is noncompetitive. Invertase I activity decreases curvilinearly with an increase in inactivator concentration. At high buffer concentrations or low inactivator concentrations in the reaction mixture, a latent period is observed when invertase I is not inactivated. Inactivation increases with an increase in temperature and a decrease in pH. 相似文献
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118.
Alice N. Neely Paul B. Nelson Gleen E. Mortimore 《Biochimica et Biophysica Acta (BBA)/General Subjects》1974,338(2):458-472
Livers from nonfasted rats were perfused in situ under conditions known from previous studies in this laboratory to increase or decrease overall endogenous proteolysis. At the termination of the experiments, lysosomal alterations were evaluated by the increase in free acid phosphatase or N-acetyl-β-D-glucosaminidase that occurred when tissue homogenates were subjected to osmotic shock in hypotonic sucrose. In control perfusions, osmotic sensitivity increased spontaneously over unperfused values, reaching maximum by 60 min or earlier. Additions of insulin, amino acid mixtures, or cycloheximide in amounts known to suppress proteolysis prevented this spontaneous perfusion effect or, when added at 60 min, rapidly reversed it. Glucagon alone during perfusion did not increase osmotic sensitivity further; however, stimulation with glucagon was observed when the perfusion effect was suppressed by insulin or cycloheximide. Anoxia, induced by gassing with nitrogen instead of oxygen, markedly reduced the perfusion effect and also doubled the amount of free acid phosphatase in the initial isotonic homogenates. Total acid phosphatase activities in the perfusion experiments were not significantly different from unperfused values and, with the exception of the anoxia perfusions, the amounts of free enzyme present in the initial isotonic sucrose homogenates did not change. 相似文献
119.
J D Nelson J J Jato-Rodriguez S Mookerjea 《Biochemical and biophysical research communications》1973,55(2):530-537
Human amniotic fluid obtained by amniocentesis during the third trimester of pregnancy was found to contain glycosyltransferases for the transfer of galactose, N-acetylgalactosamine, N-acetylglucosamine and sialic acid from their nucleotide derivatives to various exogenous protein and small molecular weight acceptors. The specific activity of the galactosyl- and N-acetylgalactosaminyl transferases was found to be 30 to 40 times higher in amniotic fluid as compared to serum. The specific activity of N-acetylglucosaminyl- and sialyl transferases was only 3 to 6 fold higher in amniotic fluid. 相似文献
120.