全文获取类型
收费全文 | 295445篇 |
免费 | 36368篇 |
国内免费 | 199篇 |
出版年
2016年 | 2933篇 |
2015年 | 4434篇 |
2014年 | 5138篇 |
2013年 | 7196篇 |
2012年 | 8199篇 |
2011年 | 8086篇 |
2010年 | 5537篇 |
2009年 | 5234篇 |
2008年 | 7334篇 |
2007年 | 7554篇 |
2006年 | 7157篇 |
2005年 | 7150篇 |
2004年 | 6916篇 |
2003年 | 7018篇 |
2002年 | 6672篇 |
2001年 | 11130篇 |
2000年 | 11208篇 |
1999年 | 9254篇 |
1998年 | 3629篇 |
1997年 | 3783篇 |
1996年 | 3755篇 |
1995年 | 3507篇 |
1994年 | 3509篇 |
1993年 | 3478篇 |
1992年 | 8215篇 |
1991年 | 8012篇 |
1990年 | 7805篇 |
1989年 | 7719篇 |
1988年 | 7281篇 |
1987年 | 7246篇 |
1986年 | 6694篇 |
1985年 | 6849篇 |
1984年 | 5748篇 |
1983年 | 5161篇 |
1982年 | 4134篇 |
1981年 | 3976篇 |
1980年 | 3602篇 |
1979年 | 5963篇 |
1978年 | 4667篇 |
1977年 | 4466篇 |
1976年 | 4264篇 |
1975年 | 4597篇 |
1974年 | 5064篇 |
1973年 | 4954篇 |
1972年 | 4609篇 |
1971年 | 4145篇 |
1970年 | 3655篇 |
1969年 | 3675篇 |
1968年 | 3285篇 |
1967年 | 2833篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
241.
Okadaic acid (OA), a protein phosphatase inhibitor, was found to induce hyperphosphorylation and reorganization of vimentin intermediate filaments in 9L rat brain tumor cells. The process was dose dependent. Vimentin phosphorylation was initially enhanced by 400 nM OA in 30 min and reached maximal level (about 26-fold) when cells were treated with 400 nM OA for 90 min. Upon removal of OA, dephosphorylation of the hyperphosphorylated vimentin was observed and the levels of phosphorylation returned to that of the controls after the cells recovered under normal growing conditions for 11 h. The phosphorylation and dephosphorylation of vimentin induced by OA concomitantly resulted in reversible reorganization of vimentin filaments and alteration of cell morphology. Cells rounded up as they were entering mitosis in the presence of OA and returned to normal appearance after 11 h of recovery. Immuno-staining with anti-vimentin antibody revealed that vimentin filaments were disassembled and clustered around the nucleus when the cells were treated with OA but subsequently returned to the filamentous states when OA was removed. Two-dimensional electrophoresis analysis further revealed that hyperphosphorylation of vimentin generated at least seven isoforms having different isoelectric points. Furthermore, the enhanced vimentin phosphorylation was accompanied by changes in the detergent-solubility of the protein. In untreated cells, the detergent-soluble and -insoluble vimentins were of equal amounts but the solubility could be increased when vimentins were hyperphosphorylated in the presence of OA. Taken together, the results indicated that OA could be involved in reversible hyperphosphorylation and reorganization of vimentin intermediate filaments, which may play an important role in the structure-function regulation of cytoskeleton in the cell. 相似文献
242.
Identification of a complex of the three forms of the rat liver asialoglycoprotein receptor 总被引:7,自引:0,他引:7
We have generated antibodies against synthetic peptides which represent the carboxyl terminus of either the major, or the two minor, forms of the rat hepatic lectin which recognizes galactose-terminated glycoproteins (asialoglycoproteins). The antibodies were shown to be specific for the form of the lectin containing the immunizing peptide sequence by the following: reaction with purified lectin after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoprecipitation of sodium dodecyl sulfate-denatured lectin, immunoprecipitation of lectin synthesized in vitro. These antibodies, however, precipitated all three rat hepatic lectin forms from nonionic detergent extracts of hepatocytes labeled with 125I via the lactoperoxidase catalyzed technique. A similar result was obtained if antibody was bound to intact cells prior to solubilization with detergent and collection of the immune complexes. We conclude that at least the plasma membrane-associated fraction of the rat hepatic lectin forms exists as a heterotypic complex. 相似文献
243.
With enzyme histochemical methods, the distribution and the activities of various oxidative and hydrolytic enzymes in the apocrine glands of the general body surface of the domestic cat were investigated. The results obtained support the view that these glands are clearly active in function. On the whole, however, the relatively weak enzyme activities in the secretory portion of the glands point to only unimportant secretion production rates. 相似文献
244.
245.
246.
247.
248.
249.
250.