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51.
Neha Pant Harold Marcotte Harald Brüssow Lennart Svensson Lennart Hammarström 《BMC microbiology》2007,7(1):86
Background
Rotavirus is a worldwide cause of infectious infantile diarrhea that claims over 600,000 lives annually. Recently, two new vaccine candidates have been developed but their efficacy in developing countries, still remains to be proven. Oral delivery of specific immunoglobulins provides passive immunity and is a fast acting treatment for rotavirus diarrhea. Probiotic bacteria have also gained considerable attention lately as treatment for rotavirus diarrhea. Here we report an evaluation of the therapeutic potential of different probiotics and their combination with anti - rotavirus antibodies in a mouse model of rotavirus diarrhea. 相似文献52.
Gehlot Ashok Chaudhary Neha Devi Jyoti Joshi Robin Kumar Dinesh Bhushan Shashi 《Plant Cell, Tissue and Organ Culture》2022,148(2):347-361
Plant Cell, Tissue and Organ Culture (PCTOC) - In vitro adventitious roots were induced from leaves of Valeriana jatamansi to assess their potential as a sustainable alternative to extract... 相似文献
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Holger Maier Christine Schütt Ralph Steinkamp Anja Hurt Elida Schneltzer Philipp Gormanns Christoph Lengger Mark Griffiths David Melvin Neha Agrawal Rafael Alcantara Arthur Evans David Gannon Simon Holroyd Christian Kipp Navis Pretheeba Raj David Richardson Sophie LeBlanc Laurent Vasseur Hiroshi Masuya Kimio Kobayashi Tomohiro Suzuki Nobuhiko Tanaka Shigeharu Wakana Alison Walling David Clary Juan Gallegos Helmut Fuchs Martin Hrabě de Angelis Valerie Gailus-Durner 《Mammalian genome》2015,26(9-10):467-481
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Uma Kumari Rishi K. Vishwakarma Neha Gupta Ruby M. V. Shirgurkar Bashir M. Khan 《Physiology and Molecular Biology of Plants》2015,21(2):261-267
Bacopa monniera is an important source of metabolites with pharmaceutical value. It has been regarded as a valuable medicinal plant and its entire commercial requirement is met from wild natural population. Recently, metabolic engineering has emerged as an important solution for sustained supply of assured and quality raw material for the production of active principles. Present report describes efficient in vitro multiplication and transformation method for genetic manipulation of this species. MS medium supplemented with 2 mgl−1 BA and 0.2 mgl−1 IAA was found optimum for maximum shoot regeneration (98.33 %) from in vitro leaves with 2–3 longitudinal cuts. Agrobacterium tumefaciens-mediated transformation method was used for generating transgenic B. monniera plants. Putative transformants were confirmed by GUS assay and PCR based confirmation of hptII gene. DNA blot analysis showed single copy insertion of transgene cassette. An average of 87.5 % of the regenerated shoots were found PCR positive for hptII gene and GUS activity was detected in leaves of transgenic shoots at a frequency of 82.5 % The efficient multiple shoots regeneration system described herein may help in mass production of B. monniera plant. Also, the high frequency transformation protocol described here can be used for genetic engineering of B. monniera for enhancement of its pharmaceutically important metabolites. 相似文献
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Bacterial spot of tomato and pepper: diverse Xanthomonas species with a wide variety of virulence factors posing a worldwide challenge
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Aher RB Wanare G Kawathekar N Kumar RR Kaushik NK Sahal D Chauhan VS 《Bioorganic & medicinal chemistry letters》2011,21(10):3034-3036
A series of dibenzylideneacetones (A1-A12) and some of their pyrazolines (B1-B4) were synthesized and evaluated in vitro for blood stage antiplasmodial properties in Plasmodium falciparum culture using SYBR-green-I fluorescence assay. The compound (1E, 4E)-1,5-bis(3,4-dimethoxyphenyl)penta-1,4-dien-3-one (A9) was found to be the most active with IC50 of 1.97 μM against chloroquine-sensitive strain (3D7) and 1.69 μM against chloroquine-resistant field isolate (RKL9). The MTT based cytotoxicity assay on HeLa cell line has confirmed that A9 is selective in its action against malaria parasite (with a therapeutic index of 166). Our results revealed that these compounds exhibited promising antiplasmodial activities which can be further explored as potential leads for the development of cheaper, safe, effective and potent drugs against chloroquine-resistant malarial parasites. 相似文献
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