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51.
Kono H Imanishi M Negi S Tatsutani K Sakaeda Y Hashimoto A Nakayama C Futaki S Sugiura Y 《FEBS letters》2012,586(6):918-923
We developed a rational scheme for designing DNA binding proteins. The scheme was applied for a zinc finger protein and the designed sequences were experimentally characterized with high DNA sequence specificity. Starting with the backbone of a known finger structure, we initially calculated amino acid sequences compatible with the expected structure and the secondary structures of the designed fingers were then experimentally confirmed. The DNA-binding function was added to the designed finger by reconsidering a section of the amino acid sequence and computationally selecting amino acids to have the lowest protein-DNA interaction energy for the target DNA sequences. Among the designed proteins, one had a gap between the lowest and second lowest protein-DNA interaction energies that was sufficient to give DNA sequence-specificity. 相似文献
52.
On the patterns of abundance and diversity of macrolichens of Chopta-Tunganath in the Garhwal Himalaya 总被引:1,自引:0,他引:1
Negi HR 《Journal of biosciences》2000,25(4):367-378
A total of 3211 colonies of macrolichens, from twelve 50 m × 10 m plots distributed across four macrohabitat (vegetation)
types between 1500 m–3700 m in the Chopta-Tunganath landscape of the Garhwal Himalaya, yielded 13 families with 15 genera
and 85 species.Lobaria retigera stood out as a broad-niched generalist species with moderate levels of abundance in all the three major microhabitats, viz.
rock, soil and wood across 83% of all the plots sampled, whereasUmbilicaria indica emerged as an abundantly occurring specialist confined to rock substrates.Heterodermia incana andLeptogium javanicum appeared to be rare members of the community as they were encountered only once during the field survey. Woody microhabitats
turned out to be richer than rock and soil substrates for macrolichens. Amongst the macrohabitats, middle altitude (2500–2800
m)Quercus forest was richest in species and genera followed by high altitude (2900–3200 m)Rhododendron forest, higher altitude grasslands (3300–3700 m) and then the lower elevation (1500 m)Quercus forest. Species, genus and family level alphaas well as beta-diversities were significantly correlated with each other, implying
that higher taxonomic ranks such as genera may be used as surrogates for species thus facilitating cost- and time-effective
periodic monitoring of the biodiversity of macrolichens. Dynamics of the diversity of lichen communities in relation to various
forms of environmental disturbance including livestock grazing and tourism as dominant land use activities in the higher Himalaya
need further research. 相似文献
53.
Certain embryological and biochemical effects of the cytokinin (SD 8339) in converting flower sex from male to hermaphrodite were studied in a clone of Vitis vinifera L. (sylvestris). The cytokinin accelerated the meiotic division of the megaspore mother cell, mitotic divisions of the megaspore and cells of pistillate tissue, and increased the rate of protein synthesis in flower buds. Two working hypotheses for the possible mode of action of the cytokinin in sex conversion are presented. 相似文献
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Calcofluor- and lectin-binding exocellular polysaccharides of Azospirillum brasilense and Azospirillum lipoferum. 总被引:2,自引:1,他引:1 下载免费PDF全文
Extracellular polysaccharides synthesized by Azospirillum brasilense and A. lipoferum were shown on agar plates and liquid flocculating cultures. The six strains used in this work expressed a mucoid phenotype, yielding positive calcofluor fluorescence under UV light. The calcofluor-binding polysaccharides were distributed between the capsular and exopolysaccharide fractions, suggesting exocellular localization. No calcofluor fluorescence was observed in residual cells after separation of the capsular and exopolysaccharide fractions. Cellulose content was significantly higher in flocculating than in nonflocculating cultures. Failure to induce flocculation by addition of cellulose (100 mg/ml) to nonflocculating cultures, together with the sensitivity of flocs to cellulase digestion, suggested that cellulose is involved in maintenance of floc stability. Different A. brasilense and A. lipoferum strains bound to a wheat lectin (fluorescein isothiocyanate-wheat germ agglutinin), indicating the occurrence of specific sugar-bearing receptors for wheat germ agglutinin on the cell surface. The biochemical specificity of the reaction was shown by hapten inhibition with N-acetyl-D-glucosamine. All six strains failed to recognize fluorescein isothiocyanate-soybean seed lectin under our experimental conditions. We conclude that azospirilla produce exocellular polysaccharides with calcofluor- and lectin-binding properties. 相似文献
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Lipid composition of whole roots of wheat (Triticum vulgare Vill. cv. Svenno Spring Wheat) and oat (Avena sativa L. cv. Brighton) and of cell wall fractions, mitochondrial fractions and microsomal fractions of these roots were studied. Lipid composition depended upon the level of mineral nutrition. In wheat total phospholipids, phosphatidyl choline and sulfolipid content was highest in the roots grown at the higher salt concentration, while the reverse was true for oat roots. In both species glycolipid and sterol content was lower in the high salt roots, at the same time as higher proportions of them were built into the microsomal fraction. Phosphatidyl choline content of the wheat root membrane fractions increased with the salt level, while the opposite occurred in the oat roots. The phosphatidyl choline content may be correlated with the (Ca2+, Mg2+)-stimulated ATPase activity. 相似文献
60.
Tanu Kaushal Gaurava Srivastava Ashok Sharma Arvind Singh Negi 《Bioorganic & medicinal chemistry》2019,27(1):16-35
Quinoxalines are benzopyrazines containing benzene and pyrazine rings fused together. In the recent past, quinoxalines have attracted Medicinal Chemists considerably for their syntheses and chemistry due to their distinct pharmacological activities. Diverse synthetic protocols have been developed via multicomponent reactions, single pot synthesis and combinatorial approach using efficient catalysts, reagents, and nano-composites etc. Further, the versatility of the quinoxaline core and its reasonable chemical simplicity devise it extremely promising source of bioactive compounds. Therefore, a wide variety of bioactive quinoxalines has been realised as antitumour, antifungal, anti-inflammatory, antimicrobial, and antiviral agents. Already, a few of them are clinical drugs while many more are under various phases of clinical trials. Present review focuses on chemistry and pharmacology (both efficacy and safety) of quinoxalines and also provides some insight in to their structure–activity relationship. 相似文献