排序方式: 共有119条查询结果,搜索用时 15 毫秒
111.
Roghayeh Pourbagher Hossein Ghorbani Haleh Akhavan-Niaki Seyed Gholam Ali Jorsaraei Sadegh Fattahi Sahar Ghooran Zeinab Abedian Masoumeh Ghasemi Fatemeh Saeedi Negar Jafari Behnam Kalali Amrollah Mostafazadeh 《Reports of Biochemistry & Molecular Biology》2021,10(1):105
Background:Stem cell differentiation therapy is a promising strategy in cancer treatment. we show that protein cocktail prepared from serum starved fibroblasts has therapeutic potential based on this strategy. Methods:The condition medium was prepared from foreskin isolated fibroblasts and analyzed by Liquid chromatography electrospray ionization mass spectrometry-mass spectrometry (LC-ESI-MS/MS). LA7 mammary gland cancer stem cells originated tumors were induced in Sprague Dawley rats. The rats treated subcutaneously with DMEM (group A), condition medium (group B), or normal saline (group C) once daily for 7 days. Then the tumors were removed and divided into the two parts, one part was used to quantify gene expression by stem-loop RT-qPCR assay and the other part was used for Hematoxylin & Eosin (H & E), Giemsa, and immunohistochemistry (IHC) staining.Results:All induced tumors appeared as sarcomatoid carcinoma (SC). Immunohistochemistry staining confirmed this conclusion by recognizing the tumor as Ki67+, cytokeratin+, vimentine+, and estrogen receptor negative SC. RT-qPCR analysis revealed that Oct4-, Sox-2, Nanog- gene expression was much reduced in the condition medium treated tumors versus proper controls (p< 0.05). Tissue necrosis was more prevalent in this group while tumors volume was diminished almost by 40%. The LC-ESI-MS/MS analysis unrevealed the stemness reducing and the cell death inducing proteins such as, pigment epithelium-derived factor (PEDF), insulin like growth factor binding protein-5 (IGFBP-5) and -7 (IGFBP-7) in the condition medium.Conclusion:This study showed that the substances released from starved human fibroblasts were able to down-regulate the stemness-related genes and induce necrosis in LA7 derived tumors.Key Words: Breast cancer, Cancer Stem cells, Cell differentiation, Fibroblasts, Gene expression 相似文献
112.
Negar Azarpira Mohamad M. Sagheb Bita Geramizadeh Masumeh Darai 《Molecular biology reports》2009,36(8):2387-2392
Objective Transplantation of renal grafts is an established treatment for renal failure in a variety of medical conditions. Polymorphisms
in genes, coding for proteins involved in immune response, may influence immunological and non-immunological mechanisms that
lead to allograft loss. Vitamin D receptor (VDR) agonist has been shown to reduce short and long term allograft rejection
in animal model. There are functional polymorphisms in VDR gene. Materials and methods A total of 75 renal allograft recipients with at least 2 years follow-up were selected and genotyped for two polymorphisms
in the VDR genes (FokI and BsmI) and the association of each genotype with renal allograft survival and acute rejection was evaluated. Results We are unable to find statistically significant association between any of the study polymorphisms and clinical outcomes.
Conclusion We have found no evidence to suggest that either VDR FokI or BsmI polymorphism determines the incidence of acute rejection or graft survival after renal transplantation. A larger sample
size is necessary to confirm these findings. 相似文献
113.
114.
Negar Azarpira Saman Nikeghbalian Bita Geramizadeh Masumeh Darai 《Molecular biology reports》2010,37(1):21-25
Glutathione-S-transferase (GST) has a major protective role against free radicals and plays a vital role in phase II of biotransformation
of many substances. In liver transplantation, reperfusion injury, calcineurin drug consumption and infection produce free
radicals that cause tissue injury and organ damage. Genetic variations of GST may influence individual susceptibility to some
diseases associated with the deleterious effects of oxidative metabolism. Although it is well known that the rejection is
an immunological process, however, in this study, we have investigated the gene frequency and relationship between human GST
gene polymorphism and rejection in liver transplant recipients. We have assessed 51 liver transplant recipients from Shiraz,
South of Iran. The GSTT1 and GSTM1 genotypes were identified by polymerase chain reaction (PCR). The gene frequency of GSTM1
and GSTT1 polymorphism were evaluated. We observed that GSTM1 null genotype was present in 68.62% of the liver transplant
recipients while GSTT1 null genotype was present in 37.25% of the liver transplant subjects. There was a trend between increasing
age and acute rejection episode. No statistically significant correlation was present between GSTM1 null and GSTT1 null genotypes
with an acute rejection episode in transplant recipients. No relationship was observed between GST genotypes and acute rejection.
It is likely that development and progression of rejection are determined by genes which is involved in immunological pathways
rather than genes that is participated in free radicals destruction. However, these findings need to be confirmed in a larger
series of patients. 相似文献
115.
Khorshidi Negar Hassanpour Halimeh Ziyadi Hakimeh 《Journal of applied phycology》2022,34(5):2283-2295
Journal of Applied Phycology - This study assessed a culture system using a static magnetic field (SMF) for improving biomass and astaxanthin production in Haematococcus lacustris. Different... 相似文献
116.
Christopher J. Quinn Todd P. Coleman Negar Kiyavash Nicholas G. Hatsopoulos 《Journal of computational neuroscience》2011,30(1):17-44
Advances in recording technologies have given neuroscience researchers access to large amounts of data, in particular, simultaneous,
individual recordings of large groups of neurons in different parts of the brain. A variety of quantitative techniques have
been utilized to analyze the spiking activities of the neurons to elucidate the functional connectivity of the recorded neurons.
In the past, researchers have used correlative measures. More recently, to better capture the dynamic, complex relationships
present in the data, neuroscientists have employed causal measures—most of which are variants of Granger causality—with limited
success. This paper motivates the directed information, an information and control theoretic concept, as a modality-independent
embodiment of Granger’s original notion of causality. Key properties include: (a) it is nonzero if and only if one process
causally influences another, and (b) its specific value can be interpreted as the strength of a causal relationship. We next describe how the causally conditioned directed information between two processes given
knowledge of others provides a network version of causality: it is nonzero if and only if, in the presence of the present
and past of other processes, one process causally influences another. This notion is shown to be able to differentiate between
true direct causal influences, common inputs, and cascade effects in more two processes. We next describe a procedure to estimate
the directed information on neural spike trains using point process generalized linear models, maximum likelihood estimation
and information-theoretic model order selection. We demonstrate that on a simulated network of neurons, it (a) correctly identifies
all pairwise causal relationships and (b) correctly identifies network causal relationships. This procedure is then used to
analyze ensemble spike train recordings in primary motor cortex of an awake monkey while performing target reaching tasks,
uncovering causal relationships whose directionality are consistent with predictions made from the wave propagation of simultaneously
recorded local field potentials. 相似文献
117.
Negar Azarpira Mehdi Dehghani Mahdokth H. Aghdaie Masumeh Darai 《Molecular biology reports》2010,37(1):27-31
Graft-versus-host disease is the main complication after hematopoietic stem cells transplantation (HSCT). Non-HLA genotypes,
such as cytokines, have been investigated for their potential roles in the occurrence and severity of GVHD as well as for
their contribution to overall transplant-related mortality and survival. IL-7 which is secreted by bone marrow stromal cells
plays an important role in the development and survival of T cells. Its effect is mediated via interleukin 7 receptor (IL7R).
This study investigates the possible links between IL-7αR single nucleotide polymorphisms (+510 C/T, +1237 A/G, +2087 T/C
and +3110A/G) and transplant outcomes among 116 recipients of HSCT. Genotypes were determined using polymerase chain reaction—sequence-specific
primers. No significant differences were observed between the genotypic distributions of IL-7αR polymorphisms and incidence
of acute or chronic graft versus host disease. Additional studies with larger sample are necessary to further define the influence
of IL-7αR on the immune response after bone marrow transplantation. 相似文献
118.
Shahsavari Gholamreza Nouryazdan Negar Adibhesami Glavizh Birjandi Mehdi 《Molecular biology reports》2020,47(7):5137-5144
Molecular Biology Reports - The oxidative modification of low-density lipoprotein (LDL) in the arterial wall plays a pivotal role in the initiation and progression of atherosclerosis which is a... 相似文献
119.
Mozaheb Negar Arefian Ehsan Aliyan Amir Amoozegar Mohammad Ali 《International microbiology》2022,25(1):165-175
International Microbiology - The field of microbial pigments is an emerging area in natural products science. Carotenoids form a major class of such pigments and are found to be diversely... 相似文献