排序方式: 共有126条查询结果,搜索用时 15 毫秒
121.
Katrina Mekhail Minhyoung Lee Michael Sugiyama Audrey Astori Jonathan St-Germain Elyse Latreille Negar Khosraviani Kuiru Wei Zhijie Li James Rini Warren L. Lee Costin Antonescu Brian Raught Gregory D. Fairn 《Journal of lipid research》2022,63(9):100256
The spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other coronaviruses mediates host cell entry and is S-acylated on multiple phylogenetically conserved cysteine residues. Multiple protein acyltransferase enzymes have been reported to post-translationally modify spike proteins; however, strategies to exploit this modification are lacking. Using resin-assisted capture MS, we demonstrate that the spike protein is S-acylated in SARS-CoV-2-infected human and monkey epithelial cells. We further show that increased abundance of the acyltransferase ZDHHC5 associates with increased S-acylation of the spike protein, whereas ZDHHC5 knockout cells had a 40% reduction in the incorporation of an alkynyl-palmitate using click chemistry detection. We also found that the S-acylation of the spike protein is not limited to palmitate, as clickable versions of myristate and stearate were also labelled the protein. Yet, we observed that ZDHHC5 was only modified when incubated with alkyne-palmitate, suggesting it has specificity for this acyl-CoA, and that other ZDHHC enzymes may use additional fatty acids to modify the spike protein. Since multiple ZDHHC isoforms may modify the spike protein, we also examined the ability of the FASN inhibitor TVB-3166 to prevent S-acylation of the spike proteins of SARS-CoV-2 and human CoV-229E. We show that treating cells with TVB-3166 inhibited S-acylation of expressed spike proteins and attenuated the ability of SARS-CoV-2 and human CoV-229E to spread in vitro. Our findings further substantiate the necessity of CoV spike protein S-acylation and demonstrate that de novo fatty acid synthesis is critical for the proper S-acylation of the spike protein. 相似文献
122.
Khorshidi Negar Hassanpour Halimeh Ziyadi Hakimeh 《Journal of applied phycology》2022,34(5):2283-2295
Journal of Applied Phycology - This study assessed a culture system using a static magnetic field (SMF) for improving biomass and astaxanthin production in Haematococcus lacustris. Different... 相似文献
123.
Christopher J. Quinn Todd P. Coleman Negar Kiyavash Nicholas G. Hatsopoulos 《Journal of computational neuroscience》2011,30(1):17-44
Advances in recording technologies have given neuroscience researchers access to large amounts of data, in particular, simultaneous,
individual recordings of large groups of neurons in different parts of the brain. A variety of quantitative techniques have
been utilized to analyze the spiking activities of the neurons to elucidate the functional connectivity of the recorded neurons.
In the past, researchers have used correlative measures. More recently, to better capture the dynamic, complex relationships
present in the data, neuroscientists have employed causal measures—most of which are variants of Granger causality—with limited
success. This paper motivates the directed information, an information and control theoretic concept, as a modality-independent
embodiment of Granger’s original notion of causality. Key properties include: (a) it is nonzero if and only if one process
causally influences another, and (b) its specific value can be interpreted as the strength of a causal relationship. We next describe how the causally conditioned directed information between two processes given
knowledge of others provides a network version of causality: it is nonzero if and only if, in the presence of the present
and past of other processes, one process causally influences another. This notion is shown to be able to differentiate between
true direct causal influences, common inputs, and cascade effects in more two processes. We next describe a procedure to estimate
the directed information on neural spike trains using point process generalized linear models, maximum likelihood estimation
and information-theoretic model order selection. We demonstrate that on a simulated network of neurons, it (a) correctly identifies
all pairwise causal relationships and (b) correctly identifies network causal relationships. This procedure is then used to
analyze ensemble spike train recordings in primary motor cortex of an awake monkey while performing target reaching tasks,
uncovering causal relationships whose directionality are consistent with predictions made from the wave propagation of simultaneously
recorded local field potentials. 相似文献
124.
Negar Azarpira Mehdi Dehghani Mahdokth H. Aghdaie Masumeh Darai 《Molecular biology reports》2010,37(1):27-31
Graft-versus-host disease is the main complication after hematopoietic stem cells transplantation (HSCT). Non-HLA genotypes,
such as cytokines, have been investigated for their potential roles in the occurrence and severity of GVHD as well as for
their contribution to overall transplant-related mortality and survival. IL-7 which is secreted by bone marrow stromal cells
plays an important role in the development and survival of T cells. Its effect is mediated via interleukin 7 receptor (IL7R).
This study investigates the possible links between IL-7αR single nucleotide polymorphisms (+510 C/T, +1237 A/G, +2087 T/C
and +3110A/G) and transplant outcomes among 116 recipients of HSCT. Genotypes were determined using polymerase chain reaction—sequence-specific
primers. No significant differences were observed between the genotypic distributions of IL-7αR polymorphisms and incidence
of acute or chronic graft versus host disease. Additional studies with larger sample are necessary to further define the influence
of IL-7αR on the immune response after bone marrow transplantation. 相似文献
125.
Shahsavari Gholamreza Nouryazdan Negar Adibhesami Glavizh Birjandi Mehdi 《Molecular biology reports》2020,47(7):5137-5144
Molecular Biology Reports - The oxidative modification of low-density lipoprotein (LDL) in the arterial wall plays a pivotal role in the initiation and progression of atherosclerosis which is a... 相似文献
126.
Mozaheb Negar Arefian Ehsan Aliyan Amir Amoozegar Mohammad Ali 《International microbiology》2022,25(1):165-175
International Microbiology - The field of microbial pigments is an emerging area in natural products science. Carotenoids form a major class of such pigments and are found to be diversely... 相似文献