全文获取类型
收费全文 | 180篇 |
免费 | 8篇 |
出版年
2023年 | 3篇 |
2022年 | 2篇 |
2021年 | 5篇 |
2020年 | 4篇 |
2019年 | 9篇 |
2018年 | 5篇 |
2017年 | 4篇 |
2016年 | 5篇 |
2015年 | 16篇 |
2014年 | 15篇 |
2013年 | 14篇 |
2012年 | 13篇 |
2011年 | 18篇 |
2010年 | 7篇 |
2009年 | 9篇 |
2008年 | 6篇 |
2007年 | 7篇 |
2006年 | 5篇 |
2005年 | 3篇 |
2004年 | 5篇 |
2003年 | 3篇 |
2002年 | 2篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 4篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1993年 | 2篇 |
1991年 | 1篇 |
1988年 | 3篇 |
1982年 | 2篇 |
1980年 | 1篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1971年 | 1篇 |
1966年 | 1篇 |
1960年 | 2篇 |
1927年 | 1篇 |
排序方式: 共有188条查询结果,搜索用时 189 毫秒
31.
Selvakumar G Mohan M Kundu S Gupta AD Joshi P Nazim S Gupta HS 《Letters in applied microbiology》2008,46(2):171-175
Aim: To determine the cold tolerance and plant growth promotion potential of Serratia marcescens strain SRM (MTCC 8708). Methods and Results: Serratia marcescens strain SRM was isolated from the flowers of summer squash plants, showing no apparent symptoms of yellow vine disease. It was evaluated for growth and plant growth promotion attributes at 15 and 4°C. At 15°C, the isolate was able to solubilize 76·6 μg ml?1 of P and produce Indole Acetic Acid, IAA (11·1 μg ml?1). HCN and siderophore production were also detected at 15°C. The isolate retained all the plant growth promotion traits at 4°C. Seed bacterization with the isolate significantly enhanced plant biomass and nutrient uptake of wheat seedlings grown in cold temperatures. Conclusion: Serratia marcescens strain SRM is a promising cold‐tolerant isolate that can significantly influence wheat seedling growth at cold temperatures. Significance and Impact of the Study: This strain can be employed as a bioinoculant in cold temperature conditions. 相似文献
32.
33.
34.
Efficient molecular markers for the selection of rapeseed genetic materials with high seed oil content and ideal fatty acid (FA) composition are preferred by rapeseed breeders. Recently, we reported the molecular mechanism of TRANSPARENT TESTA 2 (TT2) in inhibiting seed FA biosynthesis in Arabidopsis. However, evidence showing the association of rapeseed TT2 homologs and seed FA production are still insufficient. In this study, we collected 83 rapeseed (Brassica napus L.) landraces from different geographical backgrounds to conduct association mapping of BnaC.TT2.a in relation to seed coat color and FA biosynthesis. Population background was corrected by 84 pairs of SSR markers that were uniformly distributed among the linkage groups of the Tapidor-Ningyou-7 DH population. A single copy of BnaC.TT2.a for single nucleotide polymorphism (SNP) assay was cloned by a pair of previously reported specific primers. From the analysis of BnaC.TT2.a allelic variations using GLM+Q model, four SNPs on intron 1 of BnaC.TT2.a that were associated with seed FA were discovered. Moreover, an InDel at position 738 on exon 3 of BnaC.TT2.a indicated a change of protein function that was significantly associated with seed coat color, linoleic acid (C18:2), and total FA content. These findings revealed the role of BnaC.TT2.a in regulating the seed color formation and seed FA biosynthesis in rapeseed, thereby suggesting effective molecular markers for rapeseed breeding. 相似文献
35.
36.
Longhua Zhou Haoyi Wang Xin Chen Yuling Li Nazim Hussain Libo Cui Dezhi Wu Lixi Jiang 《Plant Growth Regulation》2017,83(3):385-396
37.
Influence of initial cellulose concentration on the carbon flow distribution during batch fermentation by Clostridium thermocellum ATCC 27405 总被引:1,自引:0,他引:1
The objective of this research was to understand how carbon loading influences hydrogen (H2) synthesis and metabolic flow patterns in the thermophilic, cellulolytic bacterium, Clostridium thermocellum. C. thermocellum was cultivated in batch cultures with high (5 g L−1) and low (1 g L−1) initial concentrations of α-cellulose at 60°C. The growth rate of C. thermocellum was 22% lower (0.15 h−1) in cultures with low-cellulose concentration compared with cultures with high-cellulose concentrations. Although substrate
depletion coincided with the end of log-growth in low-cellulose cultures, the prime reason for growth arrest in high-cellulose
cultures was not identified. Ethanol, acetate, and formate were the major soluble end-products with concomitant release of
H2 and CO2 under both conditions. Lactate appeared during the late log phase in high-carbon cultures when pH dropped below 6.4 and became
the major end-product in stationary phase. During the exponential phase of cell growth, significantly higher yields for H2 and acetate (1.90 ± 0.14 and 1.11 ± 0.04 mol/mol glucose equivalent, respectively) were obtained from low-cellulose cultures
compared to those from high-cellulose cultures. The maximum specific rate of H2 production, 6.41 ± 0.13 mmol H2/g dry cell/h, obtained during the exponential phase from low-carbon cultures was about 37% higher than that obtained from
high-carbon cultures. 相似文献
38.
Growth phase-dependant enzyme profile of pyruvate catabolism and end-product formation in Clostridium thermocellum ATCC 27405 总被引:1,自引:0,他引:1
Thomas Rydzak David B. Levin Nazim Cicek Richard Sparling 《Journal of biotechnology》2009,140(3-4):169-175
End-product synthesis and enzyme activities involved in pyruvate catabolism, H2 synthesis, and ethanol production in mid-log (OD600 0.25), early stationary (OD600 0.5), and stationary phase (OD600 0.7) cell extracts were determined in Clostridium thermocellum ATCC 27405 grown in batch cultures on cellobiose. Carbon dioxide, hydrogen, ethanol, acetate and formate were major end-products and their production paralleled growth and cellobiose consumption. Lactate dehydrogenase, pyruvate:formate lyase, pyruvate:ferredoxin oxidoreductase, methyl viologen-dependant hydrogenase, ferredoxin-dependant hydrogenase, NADH-dependant hydrogenase, NADPH-dependant hydrogenase, NADH-dependant acetaldehyde dehydrogenase, NADH-dependant alcohol dehydogenase, and NADPH-dependant alcohol dehydrogenase activities were detected in all extracts, while pyruate dehydrogenase and formate dehydrogenase activities were not detected. All hydrogenase activities decreased (2–12-fold) as growth progressed from early exponential to stationary phase. Alcohol dehydrogenase activities fluctuated only marginally (<45%), while lactate dehydrogenase, pyruvate:formate lyase, and pyruvate:ferredoxin oxidoreductase remained constant in all cell extracts. We have proposed a pathway involved in pyruvate catabolism and end-product formation based on enzyme activity profiles in conjunction with bioinformatics analysis. 相似文献
39.
Govindan Selvakumar Piyush Joshi Sehar Nazim Pankaj K. Mishra Jaideep K. Bisht Hari S. Gupta 《Biologia》2009,64(2):239-245
Phosphate solubilization and growth promotion by Pseudomonas fragi CS11RH1 (MTCC 8984), a psychrotolerant bacterium isolated from a high altitude garlic rhizosphere from the Indian Himalayas,
are reported here. The identity of the isolate was arrived on the basis of its biochemical features and sequencing of the
16S rRNA gene. The isolate grew and solubilized phosphate at temperatures ranging from 4 to 30°C. Besides solubilizing P it
produced indole acetic acid (IAA) and hydrogen cyanide (HCN). Seed bacterization with the isolate significantly increased
the percent germination, rate of germination, plant biomass and nutrient uptake of wheat seedlings. While Pseudomonas fragi is normally associated with the spoilage of dairy products stored at cold temperatures, this is an early report on the plant
growth promoting ability of the bacterium. 相似文献
40.
Nazim Fatès 《Swarm Intelligence》2010,4(2):91-115
The decentralised gathering problem consists in grouping in a compact cluster agents that are initially randomly scattered.
We propose a bio-inspired algorithm, the Reaction–Diffusion–Chemotaxis aggregation scheme, to group agents that have limited
abilities. The agents and their environment are described with a stochastic model inspired by the aggregation of the Dictyostelium discoideum cellular slime mold. The environment is an active lattice, whose cells transmit information according to a reaction–diffusion
mechanism. The agents are virtual amoebae; they trigger excitations randomly and move by following reaction–diffusion waves.
We demonstrate that despite its simplicity, this model exhibits interesting properties of self-organisation and is efficient
for gathering agents. Moreover, observations show that the system is robust to various perturbations, such as the presence
of obstacles on the lattice or noise in the movements of the agents. 相似文献