全文获取类型
收费全文 | 88篇 |
免费 | 10篇 |
出版年
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 1篇 |
2015年 | 5篇 |
2014年 | 3篇 |
2013年 | 4篇 |
2012年 | 2篇 |
2011年 | 2篇 |
2010年 | 3篇 |
2009年 | 4篇 |
2008年 | 1篇 |
2007年 | 2篇 |
2006年 | 3篇 |
2005年 | 4篇 |
2004年 | 3篇 |
2003年 | 2篇 |
2002年 | 3篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 3篇 |
1998年 | 4篇 |
1997年 | 1篇 |
1994年 | 3篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 4篇 |
1986年 | 3篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1975年 | 2篇 |
1973年 | 1篇 |
1970年 | 2篇 |
1968年 | 2篇 |
1967年 | 3篇 |
1966年 | 3篇 |
1964年 | 4篇 |
排序方式: 共有98条查询结果,搜索用时 15 毫秒
91.
Ana LS Vanz Gaby Renard Mario S Palma Jocelei M Chies Sérgio L Dalmora Luiz A Basso Diógenes S Santos 《Microbial cell factories》2008,7(1):13
Background
Biopharmaceutical drugs are mainly recombinant proteins produced by biotechnological tools. The patents of many biopharmaceuticals have expired, and biosimilars are thus currently being developed. Human granulocyte colony stimulating factor (hG-CSF) is a hematopoietic cytokine that acts on cells of the neutrophil lineage causing proliferation and differentiation of committed precursor cells and activation of mature neutrophils. Recombinant hG-CSF has been produced in genetically engineered Escherichia coli (Filgrastim) and successfully used to treat cancer patients suffering from chemotherapy-induced neutropenia. Filgrastim is a 175 amino acid protein, containing an extra N-terminal methionine, which is needed for expression in E. coli. Here we describe a simple and low-cost process that is amenable to scaling-up for the production and purification of homogeneous and active recombinant hG-CSF expressed in E. coli cells. 相似文献92.
93.
We report the genome sequence of a healthcare-associated MRSA type ST239 clone isolated from a patient with septicemia in Malaysia. This clone typifies the characteristics of ST239 lineage, including resistance to multiple antibiotics and antiseptics. 相似文献
94.
C. Montanelli N. Di Fonzo R. Marotta M. Motto C. Soave F. Salamini 《Molecular & general genetics : MGG》1984,197(2):209-218
Summary
o2-m(r) is an unstable allele of the O2 locus responding to the regulatory element Bg by somatic reversion. The spontaneous occurrence and the properties of the components of this system of controlling elements have been investigated. The system appears to have some degree of specificity for the O2 locus, because the majority of spontaneous O2 mutations are responsive to Bg. The component at the controlled locus undergoes frequent changes in state, while the Bg element appears more stable. Bg activity was revealed in 11 out of 108 open-pollinated varieties of maize. Most of the newly isolated Bg elements are linked with the O2 locus. The timing of induction of reversion events (which are restricted to mitotic division leading to egg or pollen maturation and to the developing endosperm) appears to correlate with the degree of linkage between Bg and the O2 locus. Germinal reversions of o2-m(r) to wild type give rise with a frequency around 5×10-4 to unstable phenotypes. Some peculiar features of the o2-m(r)-Bg system of controlling elements are discussed. 相似文献
95.
The mammalian accessory olfactory bulb (AOB) is chemoarchitecturally
heterogeneous in that it stains differentially with a number of markers;
the receptor cells that project to the AOB are similarly heterogeneous.
What is the significance of this heterogeneity? We have found that the AOB
of the gray, short-tailed opossum, Monodelphis domestica, stains
differentially with a number of 'markers': antibodies to olfactory marker
protein (OMP) and the alpha subunit of the G protein Gi2, the lectin of
Vicia villosa and NADPH-diaphorase. These markers stain the rostral AOB
more strongly than the caudal AOB whereas, the G protein subunit G(o) alpha
is located predominantly in the posterior subdivision of the AOB. This
heterogeneity in the chemoarchitecture of the AOB may reflect a fundamental
organizational dichotomy within the vomeronasal system that corresponds to
a functional dichotomy. The vomeronasal sensory epithelium also exhibits a
chemoarchitectural heterogeneity: receptor cells in the basal third are
G(o) alpha-immunoreactive whereas the cells in the middle third are Gi2
alpha-immunoreactive. Tracing studies using WGA-HRP demonstrate that the
neurons in the middle third of the vomeronasal sensory epithelium project
their axons to the anterior AOB whereas those in the basal third appear to
project to the posterior AOB.
相似文献
96.
97.
98.