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排序方式: 共有177条查询结果,搜索用时 15 毫秒
91.
92.
Daisuke Nanba Fujio Toki Sota Tate Matome Imai Natsuki Matsushita Ken Shiraishi Koji Sayama Hiroshi Toki Shigeki Higashiyama Yann Barrandon 《The Journal of cell biology》2015,209(2):305-315
Image-based identification of cultured stem cells and noninvasive evaluation of their proliferative capacity advance cell therapy and stem cell research. Here we demonstrate that human keratinocyte stem cells can be identified in situ by analyzing cell motion during their cultivation. Modeling experiments suggested that the clonal type of cultured human clonogenic keratinocytes can be efficiently determined by analysis of early cell movement. Image analysis experiments demonstrated that keratinocyte stem cells indeed display a unique rotational movement that can be identified as early as the two-cell stage colony. We also demonstrate that α6 integrin is required for both rotational and collective cell motion. Our experiments provide, for the first time, strong evidence that cell motion and epidermal stemness are linked. We conclude that early identification of human keratinocyte stem cells by image analysis of cell movement is a valid parameter for quality control of cultured keratinocytes for transplantation. 相似文献
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94.
Rattans are climbing, nonbranching palms with diverse growth forms ranging from stems that remain at the forest floor to stems
that reach the canopy. We analyzed changes in architecture and biomass allocation during ontogenetic development of 13 Indonesian
species in the genera Calamus, Ceratolobus, Daemonorops, Korthalsia, and Plectocomiopsis. Species included both nonclimbers (<5 m tall maximum) and climbers (10 to 50 m tall). Nonclimbers retain a rosette form—that
is, thick, short internodes with length/internode diameter ratio <10 but with long petioles—throughout their lives. Leaf and
internode shapes of climbers at the early stage resemble those of nonclimbers, but internodes later become longer (internode
length/diameter ratio ≥10) and petioles become shorter and developed one of two kinds of climbing organs at the adult stage.
These developmental changes reduce self-shading within the crown. Some climbers have dwarf blades at the early stage and skip
the rosette form. Principal component analysis of biomass allocation indicated that growth strategies to attain the adult
stage are diverse. These results suggest that rattans reach maturity at different phases along a series of ontogenetic development
stages and generate diverse growth forms.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
95.
96.
Hiromi Ieiri Natsuki Kameda Junko Naito Takanori Kawano Norihisa Nishida Madoka Takahashi Yoshinori Katakura 《Cytotechnology》2021,73(5):755
Euglena gracilis, a type of microalgae, contains several nutrients and accumulates paramylon, a β-1,3-glucan. In recent studies, paramylon has shown to exhibit various activities including immunomoduratory and hepatoprotective effects. In the present study, using an in vitro cell culture system, we aimed to determine whether paramylon derived from the E. gracilis EOD-1 strain, which produces large amounts of paramylon, can augment SIRT1 expression in epidermal cells via activating gut–skin interactions. Results showed that paramylon augmented the expression of SIRT1 in Caco-2 cells, a human intestinal cell line. Furthermore, microarray analysis of Caco-2 cells treated with paramylon showed that paramylon activates epidermal cells through inducing the secretion of factors from intestinal cells. Then, we focused on skin cells as target cells of paramylon-activated intestinal cells. Results showed that secretory factors from Caco-2 cells treated with paramylon augmented the expression of SIRT1 in HaCaT cells, a human keratinocyte cell line, and that expression level of genes related to the growth and maintenance of epidermal cells were significantly changed in Caco-2 cells treated with paramylon as evidenced by microarray analysis. All these results suggest that paramylon can activate epidermal cells by inducing the production of secretory factors from intestinal cells. 相似文献
97.
Masashi Shimizu Natsuki Kosaka Takashi Shimada Takemitsu Nagahata Hironori Iwasaki Hisaki Nagai Tadayoshi Shiba Mitsuru Emi 《DNA research》2002,9(5):173-178
We have devised a novel method for automated microsatellite analysis using "universal" fluorescent labeling. This system is based on polymerase chain reactions driven by sequence-specific primers and a reporter primer labeled with a fluorescent dye at its 5' end. The forward sequence-specific primer is designed with a tag region bearing no homology to any human genomic sequence. Complementary tag sequences act as templates for the 6-carboxyfluorescein-labeled reporter primer, and those products can be analyzed with an autosequencer. The results we achieved with this assay system were consistent with the results of conventional assays using radioisotope-labeled primers, and diagnosis required less time. Furthermore, the fluorescent-labeled reporter primer is "universal" in that it can be used with different sequence-specific primers designed to carry the appropriate tag sequence at their 5'-ends. Our observations suggest that the "universal" fluorescent labeling method is an efficient tool for analyzing sequence variations in human DNA. 相似文献
98.
Natsuki Kasuya Kenji Iiyama Gyosuke Meshitsuka Takeshi Okano 《Carbohydrate polymers》1997,34(4):363-234
A deoxyfluorocellulose derivative, with regioselective substitution mostly at the C-6 carbon atoms, has been studied by an X-ray diffraction method. The results showed that the crystallinity of the fluorinated cellulose is low but can be enhanced by a specific conversion treatment. The hydrothermally treated sample displayed a spectrum similar to that of cellulose IV. 相似文献
99.
100.
Natsuki Ochiai Makoto Nishizuka Tomomi Miyamoto Ichiro Miyoshi Masahito Ikawa Shigehiro Osada Masayoshi Imagawa 《Biochemical and biophysical research communications》2013
In previous studies, we identified a novel gene, factor for adipocyte differentiation 24 (fad24), which plays an important role during the early stages of adipogenesis in mouse 3T3-L1 cells. Moreover, overexpression of fad24 increased the number of smaller adipocytes in white adipose tissue and improved glucose metabolic activity in mice, thus indicating that fad24 functions as a regulator of adipogenesis in vivo. However, the physiological roles of fad24 in vivo are largely unknown. In this study, we attempted to generate fad24-deficient mice by gene targeting. No fad24-null mutants were recovered after embryonic day 9.5 (E9.5). Although fad24-null embryos were detected in an expected Mendelian ratio of genotypes at E3.5, none of the homozygous mutants developed into blastocysts. In vitro culture experiments revealed that fad24-null embryos develop normally to the morula stage but acquire growth defects during subsequent stages. The number of nuclei decreased in fad24-deficient morulae compared with that in wild-type ones. These results strongly suggested that fad24 is essential for pre-implantation in embryonic development, particularly for the progression to the blastocyst stage. 相似文献