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991.
992.
Nathalie Fenner Robert Williams Hannah Toberman Steve Hughes Brian Reynolds Chris Freeman 《Hydrobiologia》2011,665(1):51-66
The hypothesis that specific components of seawater, such as particulate, dissolved and colloidal organic and inorganic material,
render virions non-infective has long been postulated, but never rigorously tested. To address this hypothesis, the plaque
assay method was used to derive infective decay rates, k, of two bacteriophages—P1 (marine host: PWH3a) and T4 (enteric host: E. coli B). We compared k values of bacteriophage suspended in serial filtrations of seawater, with and without autoclaving and UV oxidation. Both
phages exhibited reduced decay rates in particle-free water (<0.2 μm) compared to <10 μm filtrate. The largest decrease in
virion decay rates was achieved by autoclaving the 0.2 μm filtrate. UV oxidation of <0.2 μm filtrate, however, yielded higher
decay rates than observed in autoclaved treatments. The lowest k values were seen in ultra-filtered seawater (<10 kDa). Exposure to a wide range of concentrations of Pronase E (a proteolytic
enzyme), inorganic clay (kaolinite or montmorillonite), and organic particles (phytoplankton debris) did not promote phage
inactivation. P1 infective titers were also not consistently reduced by exposures to axenic cultures of a resistant host mutant
(PWH3a-R) and a non-host marine bacterium (MB-5). Finally, phage were exposed to a range of temperatures to derive activation
energies required for phage inactivation. Application of the Arrhenius model to inactivation of T4 and P1 yielded activation
energies (E
a) of 49 and 40 kJ mol−1, respectively. This is the first comprehensive analysis in which specific seawater components were assayed for their ability
to inactivate bacteriophage. Inactivation of these phage does not appear to depend on capsomere denaturation, proteolytic
extracellular enzymes, sorption to non-host bacteria, clay particles or particulate organic debris, but is accelerated by
naturally occurring particles, which include living organisms, and heat-labile colloids and macromolecules >10 kDa. 相似文献
993.
Fort P Guémar L Vignal E Morin N Notarnicola C de Santa Barbara P Faure S 《Developmental biology》2011,(2):4511-463
The neural crest (NC) is a stem cell-like population that arises at the border of neural and non-neural ectoderm. During development, NC undergoes an epithelio-mesenchymal transition (EMT), i.e. loss of epithelial junctions and acquisition of pro-migratory properties, invades the entire embryo and differentiates into a wide diversity of terminal tissues. We have studied the implication of Rho pathways in NC development and previously showed that RhoV is required for cranial neural crest (CNC) cell specification. We show here that the non-canonical Wnt response rhoU/wrch1 gene, closely related to rhoV, is also expressed in CNC cells but at later stages. Using both gain- and loss-of-function experiments, we demonstrate that the level of RhoU expression is critical for CNC cell migration and subsequent differentiation into craniofacial cartilages. In in vitro cultures, RhoU activates pathways that cooperate with PAK1 and Rac1 in epithelial adhesion, cell spreading and directional cell migration. These data support the conclusion that RhoU is an essential regulator of CNC cell migration. 相似文献
994.
995.
The Ma gene for complete-spectrum resistance to Meloidogyne species in Prunus is a TNL with a huge repeated C-terminal post-LRR region 总被引:1,自引:0,他引:1
Claverie M Dirlewanger E Bosselut N Van Ghelder C Voisin R Kleinhentz M Lafargue B Abad P Rosso MN Chalhoub B Esmenjaud D 《Plant physiology》2011,156(2):779-792
Root-knot nematode (RKN) Meloidogyne species are major polyphagous pests of most crops worldwide, and cultivars with durable resistance are urgently needed because of nematicide bans. The Ma gene from the Myrobalan plum (Prunus cerasifera) confers complete-spectrum, heat-stable, and high-level resistance to RKN, which is remarkable in comparison with the Mi-1 gene from tomato (Solanum lycopersicum), the sole RKN resistance gene cloned. We report here the positional cloning and the functional validation of the Ma locus present at the heterozygous state in the P.2175 accession. High-resolution mapping totaling over 3,000 segregants reduced the Ma locus interval to a 32-kb cluster of three Toll/Interleukin1 Receptor-Nucleotide Binding Site-Leucine-Rich Repeat (LRR) genes (TNL1-TNL3), including a pseudogene (TNL2) and a truncated gene (TNL3). The sole complete gene in this interval (TNL1) was validated as Ma, as it conferred the same complete-spectrum and high-level resistance (as in P.2175) using its genomic sequence and native promoter region in Agrobacterium rhizogenes-transformed hairy roots and composite plants. The full-length cDNA (2,048 amino acids) of Ma is the longest of all Resistance genes cloned to date. Its TNL structure is completed by a huge post-LRR (PL) sequence (1,088 amino acids) comprising five repeated carboxyl-terminal PL exons with two conserved motifs. The amino-terminal region (213 amino acids) of the LRR exon is conserved between alleles and contrasts with the high interallelic polymorphisms of its distal region (111 amino acids) and of PL domains. The Ma gene highlights the importance of these uncharacterized PL domains, which may be involved in pathogen recognition through the decoy hypothesis or in nuclear signaling. 相似文献
996.
Both predation and individual variation in life history traits influence population dynamics. Recent results from laboratory
predator–prey systems suggest that differences between individuals can also influence predator–prey dynamics when different
genotypes experience different predation-associated mortalities. Despite the growing number of studies in this field, there
is no synthesis identifying the overall importance of the interactions between predation and individual heterogeneity and
their role in shaping the dynamics of free-ranging populations of vertebrates. We aim to fill this gap with a review that
examines how individual variability in prey susceptibility, in predation costs, in predator selectivity, and in predatory
performance, might influence prey population dynamics. Based on this review, it is clear that (1) predation risk and costs
experienced by free-ranging prey are associated with their phenotypic attributes, (2) many generalist predator populations
consist of individual specialists with part of the specialization associated with their phenotypes, and (3) a complete understanding
of the population dynamic consequences of predation may require information on individual variability in prey selection and
prey vulnerability. Altogether, this work (1) highlights the importance of maintaining long-term, detailed studies of individuals
of both predators and prey in contrasting ecological conditions, and (2) advocates for a better use of available information
to account for interactive effects between predators and their prey when modelling prey population dynamics. 相似文献
997.
von Mérey G Veyrat N Mahuku G Valdez RL Turlings TC D'Alessandro M 《Phytochemistry》2011,72(14-15):1838-1847
Maize plants respond to feeding by arthropod herbivores by producing a number of secondary plant compounds, including volatile organic compounds (VOCs). These herbivore-induced VOCs are not only known to attract natural enemies of the herbivores, but they may also prime inducible defences in neighbouring plants, resulting in stronger and faster defence responses in these VOC-exposed plants. Among the compounds that cause this priming effect, green leaf volatiles (GLVs) have received particular attention, as they are ubiquitous and rapidly emitted upon damage. In this study, we investigated their effects under realistic conditions by applying specially devised dispensers to release four synthetic GLVs at physiologically relevant concentrations in a series of experiments in maize fields. We compared the VOC emission of GLV-exposed maize plants to non-exposed plants and monitored the attraction of herbivores and predators, as well as parasitism of the caterpillar Spodoptera frugiperda, the most common herbivore in the experimental maize fields. We found that maize plants that were exposed to GLVs emitted increased quantities of sesquiterpenes compared to non-exposed plants. In several replicates, herbivorous insects, such as adult Diabrotica beetles and S. frugiperda larvae, were observed more frequently in GLV-treated plots and caused more damage to GLV-exposed plants than to non-exposed plants. Parasitism of S. frugiperda was only weakly affected by GLVs and overall parasitism rates of S. frugiperda were similar in GLV-exposed and non-exposed plots. The effects on insect presence depended on the distance from the GLV-dispensers at which the plants were located. The results are discussed in the context of strategies to improve biological control by enhancing plant-mediated attraction of natural enemies. 相似文献
998.
Simkin AJ Guirimand G Papon N Courdavault V Thabet I Ginis O Bouzid S Giglioli-Guivarc'h N Clastre M 《Planta》2011,234(5):903-914
In plants, the mevalonic acid (MVA) pathway provides precursors for the formation of triterpenes, sesquiterpenes, phytosterols
and primary metabolites important for cell integrity. Here, we have cloned the cDNA encoding enzymes catalysing the final
three steps of the MVA pathway from Madagascar periwinkle (Catharanthus roseus), mevalonate kinase (MVK), 5-phosphomevalonate kinase (PMK) and mevalonate 5-diphosphate decarboxylase (MVD). These cDNA were
shown to functionally complement MVA pathway deletion mutants in the yeast Saccharomyces cerevisiae. Transient transformations of C. roseus cells with yellow fluorescent protein (YFP)-fused constructs reveal that PMK and MVD are localised to the peroxisomes, while
MVK was cytosolic. These compartmentalisation results were confirmed using the Arabidopsis thaliana MVK, PMK and MVD sequences fused to YFP. Based on these observations and the arguments raised here we conclude that the final
steps of the plant MVA pathway are localised to the peroxisome. 相似文献
999.
Ladeuze S Lentz N Delbrassinne L Hu X Mahillon J 《Applied and environmental microbiology》2011,77(7):2555-2558
In this study, the fungistatic activity of Bacillus cereus cereulide-producing strains was demonstrated against nine fungal species. The role of cereulide was confirmed using plasmid-cured derivatives and ces knockout mutants. The fungistatic spectra of cereulide and valinomycin, a chemically related cyclododecadepsipeptide, were also compared and found to be similar but distinct. 相似文献
1000.