全文获取类型
收费全文 | 265篇 |
免费 | 14篇 |
专业分类
279篇 |
出版年
2023年 | 3篇 |
2022年 | 7篇 |
2021年 | 14篇 |
2020年 | 3篇 |
2019年 | 7篇 |
2018年 | 9篇 |
2017年 | 8篇 |
2016年 | 7篇 |
2015年 | 10篇 |
2014年 | 21篇 |
2013年 | 15篇 |
2012年 | 28篇 |
2011年 | 18篇 |
2010年 | 14篇 |
2009年 | 10篇 |
2008年 | 11篇 |
2007年 | 12篇 |
2006年 | 10篇 |
2005年 | 11篇 |
2004年 | 5篇 |
2003年 | 6篇 |
2002年 | 3篇 |
2001年 | 2篇 |
2000年 | 6篇 |
1999年 | 3篇 |
1998年 | 3篇 |
1997年 | 2篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1992年 | 2篇 |
1990年 | 1篇 |
1989年 | 6篇 |
1988年 | 5篇 |
1987年 | 2篇 |
1985年 | 1篇 |
1980年 | 4篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
排序方式: 共有279条查询结果,搜索用时 15 毫秒
51.
Manickam Sugumaran Victor Semensi Steven J. Saul 《Archives of insect biochemistry and physiology》1989,10(1):13-27
The catabolic fate of 3,4-dihydroxyphenethyl alcohol (DHPA) and 3,4-dihydroxyphenylethyl glycol (DHPG) in insect cuticle was determined for the first time using cuticular enzyme(s) from Sarcophaga bullata and compared with mushroom tyrosinase-medicated oxidation. Mushroom tyrosinase converted both DHPA and DHPG to their corresponding quinone derivatives, while cuticular enzyme(s) partly converted DHPA to DHPG. Cuticular enzyme(s)-mediated oxidation of DHPA also accompanied the covalent binding of DHPA to the cuticle. Cuticle-DHPA adducts, upon pronase digestion, released peptides that had bound catechols. 3,4-Dihydroxyphenyl-acetaldehyde, the expected product of side chain desaturation of DHPA, was not formed at all. The presence of N-acetylcysteine, a quinone trap, in the reaction mixture containing DHPA and cuticle resulted in the generation of DHPA-quinone-N-acetylcysteine adduct and total inhibition of DHPG formation. The insect enzyme(s) converted DHPG to its quinone at high substrate concentration and to 2-hydroxy-3′,4′-dihydroxyacetophenone at low concentration. They converted exogenously added DHPA-quinone to DHPG, but acted sluggishly on DHPG-quinone. These results are consistent with the enzymatic transformations of phenoloxidase-generated quinones to quinone methides and subsequent nonenzymatic transformation of the latter to the observed products. Thus, quinone methide formation in insect cuticle seems to be caused by the combined action of two enzymes, phenoloxidase and quinone tautomerase, rather than the action of quinone methide-generating phenoloxidase (Sugumaran: Arch Insect Biochem Physiol 8, 73–88, 1988). It is proposed that DHPA and DHPG in combination can be used effectively to examine the participation of (1) quinone, (2) quinone methide, and (3) dehydro derivative intermediates in the metabolism of 4-alkylcatechols for cuticular sclerotization. 相似文献
52.
Extracts prepared from dry pea (Pisum sativum, L; cv oberon) primary axes translate efficiently their endogenous messengers in an in vitro protein synthesizing system. The native long-lived messengers are biologically fully active and direct the synthesis of a whole range of polypeptides with MW ranging up to 130,000. About 0.5% of the total in vitro synthesized polypeptides are recovered in the immunoprecipitate obtained with pea lectin antiserum. Since about one-fourth of the radioactivity in the immunoprecipitate comigrates with authentic pea lectin it is concluded that about 0.1% of the long-lived messengers code for the lectin.Abbreviations mRNA
messenger RNA
- mRNP
messenger ribonucleoprotein
- SDS
sodium dodecyl sulphate
- HEPES
N-2-hydroxyethylpiperazine-N-ethanesulphonic acid
- S.A
specific activity 相似文献
53.
Kalaiselvi Senthil Murukarthick Jayakodi Pankajavalli Thirugnanasambantham Sang Choon Lee Pradeepa Duraisamy Preethi M Purushotham Kalaiselvi Rajasekaran Shobana Nancy Charles Irene Mariam Roy Arul Kumar Nagappan Gon Sup Kim Yun Sun Lee Senthil Natesan Tae-Sun Min Tae Jin Yang 《BMC genomics》2015,16(1)
54.
Pachiappan Manickam Siradanahalli C. Guru Larisa V. Debelenko Sunita K. Agarwal Shodimu-Emmanuel Olufemi Jane M. Weisemann Mark S. Boguski Judy S. Crabtree Yingping Wang Bruce A. Roe Irina A. Lubensky Zhengping Zhuang Mary Beth Kester A. Lee Burns Allen M. Spiegel Stephen J. Marx Lance A. Liotta Michael R. Emmert-Buck Francis S. Collins S. C. Chandrasekharappa 《Human genetics》1997,101(1):102-108
Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder in which affected individuals develop tumors
primarily in the parathyroids, anterior pituitary, endocrine pancreas, and duodenum. The locus for MEN1 is tightly linked to the marker PYGM on chromosome 11q13, and linkage analysis has previously placed the MEN1 gene within a 2-Mb interval flanked by markers D11S1883 and D11S449. Loss of heterozygosity (LOH) studies in MEN1 and sporadic tumors have helped narrow the location of the gene to a 600-kb
interval between PYGM and D11S449. Eighteen new polymerase chain reaction (PCR)-based polymorphic markers were generated for the MEN1 region, with ten mapping to the PYGM-D11S449 interval. These new markers, along with 14 previously known polymorphic markers, were precisely mapped on a 2.8-Mb (D11S480–D11S913) high-density clone contig-based, physical map generated for the MEN1 region.
Received: 21 February 1997 / Accepted: 5 June 1997 相似文献
55.
Beilschmiedia tirunelvelica is described and illustrated as a new species from the Western Ghats of Agasthiyamalai Biosphere Reserve, India. The differences to similar taxa are provided with dichotomous key and table. 相似文献
56.
Rosalba Lepore Andriy Kryshtafovych Markus Alahuhta Harshul A. Veraszto Yannick J. Bomble Joshua C. Bufton Alex N. Bullock Cody Caba Hongnan Cao Owen R. Davies Ambroise Desfosses Matthew Dunne Krzysztof Fidelis Celia W. Goulding Manickam Gurusaran Irina Gutsche Christopher J. Harding Marcus D. Hartmann Christopher S. Hayes Andrzej Joachimiak Petr G. Leiman Peter Loppnau Andrew L. Lovering Vladimir V. Lunin Karolina Michalska Ignacio Mir-Sanchis AK Mitra John Moult George N. Phillips Jr Daniel M. Pinkas Phoebe A. Rice Yufeng Tong Maya Topf Jonathan D. Walton Torsten Schwede 《Proteins》2019,87(12):1037-1057
The functional and biological significance of selected CASP13 targets are described by the authors of the structures. The structural biologists discuss the most interesting structural features of the target proteins and assess whether these features were correctly reproduced in the predictions submitted to the CASP13 experiment. 相似文献
57.
Comprehensive mutational analysis of yeast DEXD/H box RNA helicases involved in large ribosomal subunit biogenesis 下载免费PDF全文
Bernstein KA Granneman S Lee AV Manickam S Baserga SJ 《Molecular and cellular biology》2006,26(4):1195-1208
DEXD/H box putative RNA helicases are required for pre-rRNA processing in Saccharomyces cerevisiae, although their exact roles and substrates are unknown. To characterize the significance of the conserved motifs for helicase function, a series of five mutations were created in each of the eight essential RNA helicases (Has1, Dbp6, Dbp10, Mak5, Mtr4, Drs1, Spb4, and Dbp9) involved in 60S ribosomal subunit biogenesis. Each mutant helicase was screened for the ability to confer dominant negative growth defects and for functional complementation. Different mutations showed different degrees of growth inhibition among the helicases, suggesting that the conserved regions do not function identically in vivo. Mutations in motif I and motif II (the DEXD/H box) often conferred dominant negative growth defects, indicating that these mutations do not interfere with substrate binding. In addition, mutations in the putative unwinding domains (motif III) demonstrated that conserved amino acids are often not essential for function. Northern analysis of steady-state RNA from strains expressing mutant helicases showed that the dominant negative mutations also altered pre-rRNA processing. Coimmunoprecipitation experiments indicated that some RNA helicases associated with each other. In addition, we found that yeasts disrupted in expression of the two nonessential RNA helicases, Dbp3 and Dbp7, grew worse than when either one alone was disrupted. 相似文献
58.
59.