Application of simple fed-batch technique to high-level secretory production of insulin precursor using <Emphasis Type="Italic">Pichia pastoris</Emphasis> with subsequent purification and conversion to human insulin

Background  

The prevalence of diabetes is predicted to rise significantly in the coming decades. A recent analysis projects that by the year 2030 there will be ~366 million diabetics around the world, leading to an increased demand for inexpensive insulin to make this life-saving drug also affordable for resource poor countries.