全文获取类型
收费全文 | 494篇 |
免费 | 27篇 |
专业分类
521篇 |
出版年
2023年 | 2篇 |
2022年 | 6篇 |
2021年 | 15篇 |
2020年 | 15篇 |
2019年 | 10篇 |
2018年 | 10篇 |
2017年 | 10篇 |
2016年 | 21篇 |
2015年 | 18篇 |
2014年 | 26篇 |
2013年 | 33篇 |
2012年 | 43篇 |
2011年 | 41篇 |
2010年 | 30篇 |
2009年 | 14篇 |
2008年 | 26篇 |
2007年 | 34篇 |
2006年 | 35篇 |
2005年 | 35篇 |
2004年 | 23篇 |
2003年 | 16篇 |
2002年 | 15篇 |
2001年 | 3篇 |
2000年 | 6篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1972年 | 3篇 |
1970年 | 4篇 |
1969年 | 1篇 |
1968年 | 4篇 |
1965年 | 1篇 |
排序方式: 共有521条查询结果,搜索用时 15 毫秒
71.
72.
Salmina Alla B. Komleva Yuliya K. Malinovskaya Nataliya A. Morgun Andrey V. Teplyashina Elena A. Lopatina Olga L. Gorina Yana V. Kharitonova Ekaterina V. Khilazheva Elena D. Shuvaev Anton N. 《Biochemistry. Biokhimii?a》2021,86(6):746-760
Biochemistry (Moscow) - Blood-brain barrier (BBB) is a structural and functional element of the neurovascular unit (NVU), which includes cells of neuronal, glial, and endothelial nature. The main... 相似文献
73.
74.
Brown N Xie B Markina N Vandervelde D Perchellet JP Perchellet EM Crow KR Buszek KR 《Bioorganic & medicinal chemistry letters》2008,18(17):4876-4879
We have prepared a novel speculative eight-membered lactam demonstration library based on the skeletal structure of the potent antitumor marine natural product octalactin A. The basic scaffold was readily constructed in a convergent fashion via ring-closing metathesis chemistry from the corresponding diene amides. A cursory examination of the biological properties of the library validates the relevance and significance of these structures. 相似文献
75.
A SPOT on the chromatin landscape? Histone peptide arrays as a tool for epigenetic research 总被引:1,自引:0,他引:1
Post-translational modifications of histones serve as docking sites and signals for effector proteins and chromatin-remodeling enzymes, thereby influencing many fundamental cellular processes. Nevertheless, there are huge gaps in the knowledge of which proteins read and write the 'histone code'. Several techniques have been used to decipher complex histone-modification patterns. However, none is entirely satisfactory owing to the inherent limitations of in vitro studies of histones, such as deficits in the knowledge of the proteins involved, and the associated difficulties in the consistent and quantitative generation of histone marks. An alternative technique that could prove to be a useful tool in the study of the histone code is the use of synthetic peptide arrays (SPOT blot analysis) as a screening approach to characterize macromolecules that interact with specific covalent modifications of histone tails. 相似文献
76.
We have analysed the effect of oxidative guanine lesions on the expression of a transfected reporter gene in mouse embryonic fibroblasts deficient in Cockayne syndrome B protein (Csb) and/or the 8-oxoguanine DNA glycosylase (Ogg1). We used a highly sensitive flow cytometry-based approach and quantitative real-time PCR to measure the changes in gene expression caused by the presence of oxidised guanine residues generated by photosensitisation in the vector DNA. In wild-type cells, small numbers (one or three) of oxidised guanines did not affect gene expression at short times after transfections, whereas progressive reduction of the transgene expression was observed at later time points. Although Ogg1 has a major contribution to the repair of oxidised guanine bases, its absence did not have a strong effect on the gene expression. In contrast, the lack of functional Csb protein caused a pronounced inactivation of the damaged reporter gene. Most strikingly, an additional Ogg1 deficiency significantly attenuated this effect. The results indicate that the processing of oxidative guanine modifications by Ogg1 can mediate host cell inactivation rather than reactivation of the damaged genes and that this effect is strongly enhanced in the absence of Csb. 相似文献
77.
Erdos B Kirichenko N Whidden M Basgut B Woods M Cudykier I Tawil R Scarpace PJ Tumer N 《American journal of physiology. Heart and circulatory physiology》2011,301(1):H164-H172
Aging and obesity both have a significant impact on central blood pressure (BP) regulation, and previous studies indicated that changes in central redox signaling with age may affect high-fat (HF) diet-induced cardiovascular responses. Therefore, we investigated the effects of 60% HF feeding on BP regulation in young adult (5 mo) and old (26 mo) Fischer-344 × Brown-Norway rats. Radiotelemetric transmitters were implanted to measure BP, heart rate (HR), locomotor activity, and spontaneous baroreflex sensitivity. Expression and activity of NADPH oxidase and ANG II type 1 receptor were assessed in the hypothalamus and in the nucleus tractus solitarii. Old animals gained more weight on HF diet compared with young, whereas central NADPH oxidase expression and activity elevated similarly in the two age groups. After an initial hypotensive and tachycardic response during the first week of HF feeding, BP in young animals increased and became significantly elevated after 6 wk of HF feeding. In contrast, BP in old animals remained depressed. Nighttime HR and locomotor activity decreased in both young and old rats fed with HF diet, but these changes were more significant in young rats. As a result, amplitudes of circadian variation of BP, HR, and activity that were originally higher in young rats declined significantly and became similar in the two age groups. In conclusion, our experiments led to the surprising finding that HF diet has a more serious impact on cardiovascular regulation in young animals compared with old. 相似文献
78.
79.