排序方式: 共有77条查询结果,搜索用时 718 毫秒
51.
Krstić NM Bjelaković MS Pavlović VD Robeyns K Juranić ZD Matić I Novaković I Sladić DM 《Steroids》2012,77(5):558-565
The reactions of 17α-hydroxyprogesterone with Lawesson's reagent (LR) in toluene, CH(2)Cl(2) and/or CCl(4) gave, depending on the duration of the reaction, two diastereoisomeric androst-4-en-17-spiro-1,3,2-oxathiaphospholane-2-sulfide pairs 2a,b and 3a,b in approximately 7:3 ratio, differing in configuration at the phosphorus atom. A parallel analysis of heteronuclear 2D (1)H-(13)C spectra (HSQC and HMBC) and homonuclear 2D spectra (NOESY) enabled complete (1)H and (13)C assignments of each isomer. Also, analysis of NOESY correlations provided evidence for the preferred conformation. X-ray analysis of 3a confirmed the structure and absolute configuration on phosphorus. A pathway for the formation of 1,3,2-oxathiaphospholane ring was proposed. Cytotoxic activity in vitro was tested against three tumor cell lines (human cervix carcinoma HeLa cells and two human breast carcinoma MDA-MB-361 and MDA-MB-453 cells). Compound 3a and mixture 3a,b showed a moderate activity against HeLa and MDA-MB-453 cell lines while against MDA-MB-361 cell line all tested compounds exerted very weak cytotoxic effect. Antimicrobial activity against Gram-positive, Gram-negative bacteria and fungal cells, toxicity to brine shrimp Artemia salina, were evaluated. All tested compounds showed strong antifungal activity. 相似文献
52.
53.
Fehlberg V Vieweg MF Dohmann EM Hohnjec N Pühler A Perlick AM Küster H 《Journal of experimental botany》2005,56(413):799-806
In this study the further characterization of the Vicia faba leghaemoglobin promoter pVfLb29 is presented that was previously shown to be specifically active in the infected cells of root nodules and in arbuscule-containing cells of mycorrhizal roots. Using promoter studies in transgenic hairy roots of the Pisum sativum mutant RisNod24, disabled in the formation of functional arbuscules, VfLb29 promoter activity is assigned to later stages of arbuscule development. In order to narrow down the regions containing cis-acting elements of pVfLb29, the activity of five VfLb29 promoter deletions (-797/-31 to -175/-31 in relation to the start codon) fused to the gusAint coding region were tested in transgenic V. hirsuta hairy roots. The results specify a promoter region ranging from position -410 to -326 (85 bp) as necessary for gus expression in arbuscule-containing cells, whereas this segment is not involved in the nodule-specific activity. Sequence analysis of the pVfLb29 fragment -410/-326 (85 bp) revealed sequence motifs previously shown to be cis-acting elements of diverse promoters. To investigate the autonomous function of pVfLb29 regions for activation in arbuscule-containing cells, different regions of pVfLb29 from positions -410 to -198 were used to prepare chimeric promoter constructs for trans-activation studies. These fragments alone did not activate the mycorrhiza inactive promoter of the Vicia faba leghaemoglobin gene VfLb3, showing that the activation of pVfLb29 in arbuscule-containing cells is governed by a complex regulatory system that requires at least two modules located between position -410 and -31 of the VfLb29 gene. 相似文献
54.
55.
Maja Mizdrak Katarina Vukojević Natalija Filipović Vesna Čapkun Benjamin Benzon Merica Glavina Durdov 《Diagnostic pathology》2018,13(1):90
Background
In glomerular injury dendrin translocates from the slit diaphragm to the podocyte nucleus, inducing apoptosis. We analyzed dendrin expression in IgA glomerulonephritis and Henoch Schönlein purpura (IgAN/HSP) versus in podocytopathies minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS), and compared it to pathohistological findings and renal function at the time of biopsy and the last follow-up.Methods
Twenty males and 13 females with median of age 35?years (min-max: 3–76) who underwent percutaneous renal biopsy and had diagnosis of glomerular disease (GD) were included in this retrospective study. Fifteen patients had IgAN/HSP and eighteen podocytopathy. Control group consisted of ten patients who underwent nephrectomy due to renal cancer. Dendrin expression pattern (membranous, dual, nuclear or negative), number of dendrin positive nuclei and proportion of dendrin negative glomeruli were analyzed.Results
In GD and the control group significant differences in number of dendrin positive nuclei and proportion of dendrin negative glomeruli were found (P?=?0.004 and P?=?0.003, respectively). Number of dendrin positive nuclei was higher in podocytopathies than in IgAN/HSP, 3.90 versus 1.67 (P?=?0.028). Proportion of dendrin negative glomeruli correlated to higher rates of interstitial fibrosis (P?=?0.038), tubular atrophy (P?=?0.011) and globally sclerotic glomeruli (P?=?0.008). Dual and nuclear dendrin expression pattern were connected with lower rate of interstitial fibrosis and tubular atrophy than negative dendrin expression pattern (P?=?0.024 and P?=?0.017, respectively). Proportion of dendrin negative glomeruli correlated with lower creatinine clearance (CC) at the time of biopsy and the last follow-up (P?=?0.010 and P?<?0.001, respectively). Dendrin expression pattern correlated to CC at the last follow-up (P?=?0.009), being lower in patients with negative than nuclear or dual dendrin expression (P?=?0.034 and P?=?0.004, respectively).Conclusion
In this pilot study the number of dendrin positive nuclei was higher in podocytopathies than in inflammatory GD. Negative dendrin expression pattern correlated to chronic tubulointerstitial changes and lower CC, which needs to be confirmed in a larger series.56.
Histone Modifications Pattern Associated With a State of Mesenchymal Stem Cell Cultures Derived From Amniotic Fluid of Normal and Fetus‐Affected Gestations
下载免费PDF全文
![点击此处可从《Journal of cellular biochemistry》网站下载免费的PDF全文](/ch/ext_images/free.gif)
57.
Ge Y Domschke C Stoiber N Schott S Heil J Rom J Blumenstein M Thum J Sohn C Schneeweiss A Beckhove P Schuetz F 《Cancer immunology, immunotherapy : CII》2012,61(3):353-362
Severe immune suppression is frequent in late-stage tumor patients and promotes tumor immune evasion and subsequent tumor progression. Regulatory T cells (Treg) are major suppressors of anti-tumor immune responses. Therefore, targeting of Treg has become a key goal of anti-tumor therapy. Several preclinical and clinical observations suggest that Treg can be depleted by cyclophosphamide. Over a period of 3?months, we investigated the effect of metronomic low-dose cyclophosphamide on Treg numbers, suppressive capacity and proliferation on endogenous anti-tumor T-cell responses and on their correlation to clinical outcome in 12 patients with treatment-refractory metastasized breast cancer who received single-agent 50?mg cyclophosphamide p.o. daily. Cyclophosphamide treatment initially caused a significant reduction in circulating Treg by more than 40% (P?=?0.002). However, Treg numbers completely recovered during the treatment due to increased proliferative activity and maintained their suppressive capacity. Treg depletion coincided with a strong increase in breast tumor-reactive T cells (P?=?0.03) that remained at high levels during the whole period. Numbers of tumor-reactive T cells but not of Treg correlated with disease stabilization (P?=?0.03) and overall survival (P?=?0.027). We conclude that metronomic low-dose cyclophosphamide only transiently reduces Treg but induces stable tumor-specific T-cell responses, which correlate with improved clinical outcome in advanced-stage breast cancer patients. 相似文献
58.
Pasalić D Marinković N Grsković B Ferencak G Bernat R Stavljenić-Rukavina A 《Molecular biology reports》2009,36(4):775-780
Human C-reactive protein (CRP) is a reactant involved in the acute phase response and one of the many molecular factors involved
in pathogenesis of coronary artery disease (CAD). CRP gene variants potentially mediate CRP plasma concentrations and the
development of CAD. 220 Croatian subjects with angiographically confirmed CAD and 132 control subjects were included in the
study. CRP gene polymorphisms 1059G/C and -717G/A were determined by RFLPs, using MaeIII and KspI endonuclease, respectively. Plasma concentrations of CRP and homocysteine were determined by immunoturbidimetry and FPIA,
respectively. CRP 1059G/C gene variants were significantly associated with CAD (OR = 0.50; 95% CI = 0.27, 0.94; P = 0.032). Wild GG genotype and rare allele C carrier genotypes were 184 and 22 in CAD(+) group, and 101 and 24 in CAD(−)
group, respectively. Multivariate analysis with age, gender, BMI, smoking status, hypertension and diabetes as covariates
showed that 1059C carriers had lower CRP concentrations in CAD(−) (P = 0.010) and CAD(+) subjects (P = 0.028). This allele was also significantly associated with lower plasma homocysteine concentrations in both groups (P = 0.018 for CAD(−) and 0.002 for CAD(+). There was no significant difference between CAD(+) and CAD(−) subjects in absolute
frequencies for CRP -717A/G gene variant, but multivariate analysis showed that carriers of the rarer G allele had significantly
higher CRP plasma concentrations in CAD(−) subjects (P = 0.031) and higher homocysteine concentrations in CAD(+) group (P < 0.001). Atherosclerosis is an inflammatory disease resulting from different genetic and environmental factors. Results
presented here support the contribution of CRP genetic variations in the development of CAD. 相似文献
59.
Schuetz F Ehlert K Ge Y Schneeweiss A Rom J Inzkirweli N Sohn C Schirrmacher V Beckhove P 《Cancer immunology, immunotherapy : CII》2009,58(6):887-900
Background Breast cancer patients frequently harbour tumour-reactive memory T cells in their bone marrow (BM) but not in the blood. After
reactivation ex-vivo these cells rejected autologous breast tumours in xenotransplanted mice demonstrating therapeutic potential
upon reactivation and mobilization into the blood. We conducted a clinical pilot study on metastasized breast cancer patients
to investigate if ex-vivo reactivation of tumour-reactive BM memory T cells and their adoptive transfer is feasible and increases
the frequencies of tumour-reactive T cells in the blood.
Methods The study protocol involved one transfusion of T cells which were reactivated in vitro with autologous dendritic cells pulsed
with lysate of MCF7 breast cancer cells as source of tumour antigens. Immunomonitoring included characterization of T cell
activation in vitro and of tumour-specific T cells in the blood by interferon (IFN)-γ ELISPOT assay, HLA-tetramers and antigen-induced
interleukin (IL)-4 secretion.
Results Twelve patients with pre-existing tumour-reactive BM memory T cells were included into the study. In all cases, the treatment
was feasible and well tolerated. Six patients (responders) showed by ELISPOT assay de-novo tumour antigen-specific, IFN-γ-secreting
T cells in the blood after 7 days. In contrast, non responders showed in the blood tumour antigen-induced IL-4 responses.
All responders received more than 6.5 × 103 tumour-reactive T cells. In contrast, all non responders received lower numbers of tumour antigen-reactive T cells. This
was associated with reduced activation of memory T cells in activation cultures, increased amounts of CD4+ CD25high regulatory T cells in the BM and increased tumour antigen-dependent IL-10 secretion. The latter was prevented by preceding
depletion of regulatory T cells suggesting that regulatory T cells in the BM can inhibit reactivation of tumour-specific T
cells.
Conclusion Taken together, adoptive transfer of ex-vivo re-stimulated tumour-reactive memory T cells from BM of metastasized breast cancer
patients can induce the presence of tumour antigen-reactive type-1 T cells in the peripheral blood.
Florian Schuetz and Katrin Ehlert contributed equally to the study. 相似文献
60.
The objectives of this study were to investigate impact of stump and slash removal on growth and mycorrhization of Picea abies seedlings outplanted on a forest clear-cut. Four non-replicated site preparation treatments included: (1) mounding (M), (2)
removal of stumps (K), (3) mounding and removal of logging slash (HM) and (4) removal of logging slash and stumps (HK). Results
showed that height increment of the seedlings was highest in K and lowest in M after the third growing season, and similar
pattern remained after the fourth season. Ectomycorrhizal (ECM) colonisation of seedling roots was highest in M (96.6%) and
lowest in K (72.3%), and even in HK (76.0%) and HM (76.3%). Morphotyping and sequencing of internal transcribed spacer of
fungal ribosomal DNA revealed a total of 13 ECM species. Among those, Thelephora terrestris and Cenococcum geophilum were the most common, found on 27.4% and 26.3% of roots, respectively. The rest of species colonised 26.6% of roots. Richness
of ECM species was highest in M (10 species) and lowest in K (three species). Consequently, stump and slash removal from clear-felled
sites had a positive effect on growth of outplanted spruce seedlings, but negative effect on their mycorrhization. This suggests
that altered soil conditions due to site disturbance by stump and slash removal might be more favourable for tree growth than
more abundant mycorrhization of their root systems in less disturbed soil. 相似文献