Assessment of Environmental Hazards to Public Health in Temperate Urban Argentina

EcoHealth - Human health risk in urban areas depends on multiple environmental features. We performed a year-round survey in a highly urbanized district located in temperate Argentina (General San...     

Alendronate inhibits triglyceride accumulation and oxidative stress in adipocytes and the inflammatory response of macrophages which are associated with adipose tissue dysfunction

Journal of Physiology and Biochemistry - Alendronate, a bisphosphonate used to prevent osteoporosis, stimulates osteogenesis but impairs adipogenesis. Different clinical trials suggest that the...     

A Comparison of Vegetation and Ground‐Dwelling Ants in Abandoned and Restored Gullies and Landslide Surfaces in the Western Colombian Andes

Landslides and gullies are two common manifestations of land degradation in the densely populated Colombian Andes. In these unstable areas, further mass movements pose a serious threat to local populations and cause off‐site environmental damage through sedimentation, pollution, and increased flooding. A novel approach for restoring severely eroded slopes combines the use of stabilization structures made with stalks of Guadua angustifolia Kunth, Poaceae (bamboo), with high‐density planting of species that exhibit quick growth and sprouting. This study compared the vegetation and ground‐dwelling ant assemblages of 10 pairs of gullies, each pair formed by one enhanced and one untreated or control gully, 6–8 years after restoration or abandonment. The restoration treatment had significant effects on the complexity of vegetation. Average values for plant species richness, basal area, stem density, foliage density index, and total vegetation volume were 11.6, 140, 30, 11.5, and 15.6 times larger, respectively, in enhanced than in control gullies. Mirroring the differences in vegetation, average ant species richness was significantly larger (13 vs. 7.6 species per gully), and a higher proportion of ant species nested within enhanced than control gullies (52.5 vs. 30%). While control gullies were dominated by generalist ants such as Ectatomma ruidum and Linepithema angulatum, enhanced gullies had more specialized ground‐dwelling species, normally associated with high plant cover and abundant leaf litter such as Octostruma balzani and Heteroponera inca. We conclude that this restoration strategy promotes a fast recovery of vegetation and the ground‐dwelling ant fauna in these tropical mountains.     

The Evolution of the Cenozoic Terrestrial Mammalian Predator Guild in South America: Competition or Replacement?

South America was isolated from other continents during most of the Cenozoic, developing a singular mammalian fauna. In contrast to North America, Europe, Asia, and Africa, up to the late Neogene, the carnivore adaptive zone in South America was populated by crocodiles (Sebecidae), large snakes (Madtsoiidae), large birds (Phorusrhacidae), and metatherian mammals (Sparassodonta). Sparassodonta were varied and comprised a wide range of body masses (≈ 2–50 kg) and food habits. Their diversity decreased towards the late Miocene (Huayquerian Stage/Age) and the group became extinct in the “middle” Pliocene (≈ 3 Ma, Chapadmalalan Stage/Age). Several authors have suggested that the cause of this decline and extinction was the ingression of carnivorans to South America (about 6–7 Ma ago), because they competed with the Sparassodonta; although this hypothesis has been criticized in recent years. With the intention of testing the hypothesis of “competitive displacement,” we review the fossil record of South American Sparassodonta and Carnivora, collect data about diversity, estimate size and diet, and determine first and last appearances. The diversity of Sparassodonta is low relative to that of Carnivora throughout the Cenozoic with the early Miocene (Santacrucian Stage/Age) showing the greatest diversity with 11 species. In the late Miocene-middle Pliocene (Huayquerian Stage/Age), the fossil record shows overlap of groups, and the Sparassodonta’s richness curve begins to decline with the first record of Carnivora. Despite this overlap, carnivorans diversity ranged from four or fewer species in the late Miocene-Pliocene to a peak of around 20 species in the early Pleistocene (Ensenadan Stage/Age). Carnivora was initially represented by small-sized, omnivorous species, with large omnivores first appearing in the Chapadmalalan Stage/Age. Over this period, Sparassodonta was represented by large and small hypercarnivores and a single large omnivorous species. From this review of the fossil record, it is suggested that factors other than competitive displacement may have caused the extinction of the Sparassodonta.     

S-nitrosoglutathione-induced toxicity in Drosophila melanogaster: Delayed pupation and induced mild oxidative/nitrosative stress in eclosed flies

The toxicity of the nitric oxide donor S-nitrosoglutathione (GSNO) was tested on the Drosophila melanogaster model system. Fly larvae were raised on food supplemented with GSNO at concentrations of 1.0, 1.5 or 4.0 mM. Food supplementation with GSNO caused a developmental delay in the flies. Biochemical analyses of oxidative stress markers and activities of antioxidant and associated enzymes were carried out on 2-day-old flies that emerged from control larvae and larvae fed on food supplemented with GSNO. Larval exposure to GSNO resulted in lower activities of aconitase in both sexes and also lower activities of catalase and isocitrate dehydrogenase in adult males relative to the control cohort. Larval treatment with GSNO resulted in higher carbonyl protein content and higher activities of glucose-6-phosphate dehydrogenase in males and higher activities of superoxide dismutase and glutathione-S-transferase in both sexes. Among the parameters tested, aconitase activity and developmental end points may be useful early indicators of toxicity caused by GSNO.     

Free-radical Scavengers and Antioxidants from Peumus boldus Mol. ("Boldo")

The dry leaves of Peumus boldus (Monimiaceae) are used in infusion or decoction as a digestive and to improve hepatic complains. Preliminary assays showed free-radical scavenging activity in hot water extracts of boldo leaves, measured by the decoloration of a methanolic solution of the 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH). Assay-guided isolation led to the active compounds. Catechin proved to be the main free-radical scavenger of the extracts. Lipid peroxidation in erythrocytes was inhibited by boldo extracts and fractions at 500 &#119 g/ml with higher effect for the ethyl acetate soluble and alkaloid fractions. The IC 50 for catechin and boldine in the lipid peroxidation test were 75.6 and 12.5 &#119 g/ml, respectively. On the basis of dry starting material, the catechin content in the crude drug was 2.25% while the total alkaloid calculated as boldine was 0.06%. The activity of boldine was six times higher than catechin in the lipid peroxidation assay. However, the mean catechin:total alkaloid content ratio was 37:1. The relative concentration of alkaloids and phenolics in boldo leaves and their activity suggest that free-radical scavenging effect is mainly due to catechin and flavonoids and that antioxidant effect is mainly related with the catechin content. The high catechin content of boldo leaves and its bioactivity suggest that quality control of Boldo folium has to combine the analysis of catechin as well as their characteristic aporphine alkaloids.