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排序方式: 共有210条查询结果,搜索用时 15 毫秒
161.
Enrih Merdi? ?eljka Jeli?i? Stjepan Kr?mar Branimir Hackenberger-Kutuzovi? Nata?a Turi? Mirta Sudari? Bogojevi? ?eljko Zahirovi? 《Biologia》2010,65(3):545-551
Efficacy of different mosquito attractants was invesigated at four sites in three plant communities (Galio-Salicetum albae, Populetum nigro-albae, Genisto elatae-Quercetum roboris) of flooded and forest habitats in Kopački rit Nature Park, Croatia. The attractants were: dry ice, horse urine, horse urine
+ acetone, acetone, 1-octen-3-ol and ammonium hydroxide baited CDC traps. A total of 11,441 mosquito specimens of 12 species
were collected. Aedes vexans (91.43%) was the most numerous species. A statistically significant difference between the efficacy of dry ice and the other
attractants was shown, whereas there was no difference between the other attractants. A greater number of specimens and species
number were noted in the flooded plant communities (Populetum nigro-albae). The response of Ae. vexans to dry ice was higher in flooded sites, and it was significantly lower in forest habitat (Genisto elatae-Quercetum roboris) according to fuzzy c-means cluster analysis. The same analysis shows a higher efficacy of other attractants (horse urine,
horse urine + acetone, acetone, octenol and ammonium hydroxide) in forest habitat when compared to the flooded area habitats. 相似文献
162.
Irena Sović Samy Jambon Sandra Kraljević Pavelić Elitza Markova-Car Nataša Ilić Sabine Depauw Marie-Hélène David-Cordonnier Grace Karminski-Zamola 《Bioorganic & medicinal chemistry》2018,26(8):1950-1960
In this paper novel isoindolines substituted with cyano and amidino benzimidazoles and benzothiazoles were synthesized as new potential anti-cancer agents. The new structures were evaluated for antiproliferative activity, cell cycle changes, cell death, as well as DNA binding and topoisomerase inhibition properties on selected compounds. Results showed that all tested compounds exerted antitumor activity, especially amidinobenzothiazole and amidinobenzimidazole substituted isoindolin-1-ones and benzimidazole substituted 1-iminoisoindoline that showed antiproliferative effect in the submicromolar range. Moreover, the DNA-binding properties of selected compounds were evaluated by biophysical and biochemical approaches including thermal denaturation studies, circular dichroism spectra analyses and topoisomerase I/II inhibition assays and results identified some of them as strong DNA ligands, harboring or not additional topoisomerase II inhibition and able to locate in the nucleus as determined by fluorescence microscopy. In conclusion, we evidenced novel cyano- and amidino-substituted isoindolines coupled with benzimidazoles and benzothiazoles as topoisomerase inhibitors and/or DNA binding compounds with potent antitumor activities. 相似文献
163.
Matjaž Ravnikar Andreja Irman Nataša Radić Mojca Lunder Borut Štrukelj 《Biotechnology letters》2009,31(12):1943-1946
A new method for fast transformation of competent bacterial cells has been developed. The transformation is induced with cholic
acid analogues or saponins which cause reversible disruption of the bacterial membrane. This method shortens the time of transformation
without significant loss of transformation efficiency in comparison to heat shock method and is the first reported chemically-induced
transformation. New data about interactions between cholates and biomembranes is revealed that may contribute to better understanding
of bacterial transformation. 相似文献
164.
Jun Sakamoto Mikihiro Fujiya Kotaro Okamoto Toshie Nata Yuhei Inaba Kentaro Moriichi Hiroki Tanabe Yusuke Mizukami Jiro Watari Toshifumi Ashida Yutaka Kohgo 《Cancer epidemiology》2010,34(2):194-199
Background: We developed a novel method of methylation-specific PCR (MSP) using immunoprecipitation with anti-histone antibody (IP-MSP) to efficiently detect serum methylated DNA tightly bound to de-acetylated histones. Materials and methods: The detection limit of IP-MSP for p16 methylation was determined with a standard made by cell line (SKCO-1) lysate. p16 methylation of tumor and/or serum of 51 colorectal cancers and 10 adenoma patients, and 10 healthy volunteers was detected with conventional MSP or IP-MSP. Results: IP-MSP detected p16 methylation from 0.5 pg/μl of the cell lysate. The sensitivity of IP-MSP for detecting serum p16 methylation in 27 patients with tumors characterized by p16 methylation was significantly higher than that with conventional method (81% versus 59%), particularly in Stage II patients (91% versus 45%). IP-MSP detected no p16 hypermethylation in sera of adenoma patients and volunteers. Conclusions: IP-MSP is thus considered to be a promising procedure to detect serum methylated DNA in colorectal cancer patients. 相似文献
165.
Du?an Mladenovi? Nata?a Petronijevi? Tihomir Stojkovi? Milica Velimirovi? Gordana Jevti? Dragan Hrn?i? Tatjana Radosavljevi? Aleksandra Ra?i?-Markovi? Neboj?a Maksi? Dragan Djuric Olivera Stanojlovi? 《PloS one》2015,10(8)
Finasteride (FIN) inhibits neurosteroid synthesis and potentially improves the course of hepatic encephalopathy (HE). This study aimed to investigate the effects of FIN on brain oxidative stress and acetylcholinesterase (AchE) activity in acute thioacetamide-induced HE in rats. Male Wistar rats were divided into groups: 1. control; 2. thioacetamide-treated group (TAA; 900 mg/kg); 3. finasteride-treated group (FIN; 150 mg/kg); 4. group treated with FIN and TAA (FIN+TAA). Daily doses of FIN (50 mg/kg) and TAA (300 mg/kg) were administered intraperitoneally during three days and in FIN+TAA group FIN was administered 2h before every dose of TAA. FIN pretreatment prevented TAA-induced rise in malondialdehyde level in the cortex due to restoration of catalase activity and increased expression of superoxide dismutase 1 (SOD1) and induced an increase in malondialdehyde level in the thalamus due to reduction of glutathione peroxidase (GPx) and glutathione reductase (GR) activity. Although FIN pretreatment did not affect malondialdehyde level in hippocampus and caudate nucleus, hippocampal SOD1 expression was higher (p<0.05) and GR activity lower in FIN+TAA vs. TAA group (p<0.05). GPx activity was lower in caudate nucleus in FIN+TAA vs. TAA group (p<0.01). FIN pretreatment prevented TAA-induced rise in AchE activity in the thalamus and caudate nucleus and AchE activity correlates inversely in the thalamus (p<0.05) and positively in caudate nucleus (p<0.01) with malondialdehyde level. FIN has regionally selective effects on oxidative stress and AchE activity in the brain in acute TAA-induced HE in rats. The prooxidant role of FIN in the thalamus may be causally linked with inhibition of AchE. 相似文献
166.
Uro? Klan?ar Sa?a Baumgartner Igor Legen Polona Smrdel Nata?a Jeraj Kampu? Dejan Krajcar Bo?tjan Markun Klemen Ko?evar 《AAPS PharmSciTech》2015,16(2):398-406
It is challenging to achieve mechanically robust drug-release profiles from hydrophilic matrices containing a high dose of a drug with good solubility. However, a mechanically robust drug release over prolonged period of time can be achieved, especially if the viscosity and amount of the polymer is sufficiently high, above the “threshold values.” The goal of this research was to determine the hydroxypropyl cellulose (HPC) and hydroxypropyl methylcellulose (HPMC) polymer threshold amount that would enable robust drug release from matrix tablets containing a high dose of levetiracetam as a class I model drug according to the Biopharmaceutical Classification System (BCS). For this purpose, formulations containing HPC or HPMC of similar viscosity range, but in different amounts, were prepared. Based on the dissolution results, two final formulations were selected for additional in vitro and in vivo evaluation to confirm the robustness and to show bioequivalence. Tablets were exposed to various stress conditions in vitro with the use of different mechanically stress-inducing dissolution methods. The in vitro results were compared with in vivo results obtained from fasted and fed bioequivalence studies. Under both conditions, the formulations were bioequivalent and food had a negligible influence on the pharmacokinetic parameters Cmax and area under the curve (AUC). It was concluded that the drug release from both selected formulations is mechanically robust and that HPC and HPMC polymers with intrinsic viscosities above 9 dL/g and in quantities above 30% enable good mechanical resistance, which ensures bioequivalence. In addition, HPC matrices were found to be more mechanically robust compared to HPMC.KEY WORDS: HPC, HPMC, matrix tablets, mechanically robust dissolution, threshold amount 相似文献
167.
168.
169.
The adhesion molecule lymphocyte function-associated antigen (LFA)-1 plays a key role in immune surveillance and response. Its conformation is spatially and temporally regulated, enabling adhesion and deadhesion during T-cell migration. LFA-1 adhesion to its major ligand intercellular adhesion molecule 1 is controlled by adaptor proteins which bind the cytoplasmic tail of the β (2) subunit. Cathepsin X, a cysteine carboxypeptidase, promotes T-cell migration and morphological changes by cleaving the β (2) cytoplasmic tail of LFA-1. In this way, it modulates the affinity of LFA-1 for structural adaptors talin-1 and α-actinin-1 and enables the stepwise transition between intermediate and high-affinity conformations of LFA-1, an event that is necessary for effective T-cell function. Cathepsin X regulation that would allow precise modulation of LFA-1 affinity has a great potential for anti-LFA-1 therapy. 相似文献
170.
Todorovic-Rakovic N 《Journal of biosciences》2011,36(4):719-724
The current state in oncology research indicates that the attempts to explain such complex process as cancerogenesis by a
single or several genetic mutations were not successful enough. On the other hand, chromosomal/genomic instability – almost
universal features of malignant tumours which influence a global pattern of gene expression and, subsequently, many oncogenic
pathways – were often disregarded and considered nonessential to clinical application. However, a new arising field of system
biology including ‘new forms’ of genome diversity such as copy number variations (CNV) and high-throughput oncogene mutation
profiling now reveal all the complexity of cancer and provide the final explanation of the oncogenic pathways, based on stochastic
(onco)genomic variation rather than on (onco)genic concepts. 相似文献