全文获取类型
收费全文 | 279篇 |
免费 | 20篇 |
国内免费 | 1篇 |
出版年
2023年 | 3篇 |
2022年 | 10篇 |
2021年 | 20篇 |
2020年 | 12篇 |
2019年 | 28篇 |
2018年 | 18篇 |
2017年 | 13篇 |
2016年 | 17篇 |
2015年 | 24篇 |
2014年 | 20篇 |
2013年 | 17篇 |
2012年 | 22篇 |
2011年 | 13篇 |
2010年 | 11篇 |
2009年 | 5篇 |
2008年 | 11篇 |
2007年 | 8篇 |
2006年 | 13篇 |
2005年 | 6篇 |
2004年 | 11篇 |
2003年 | 4篇 |
2002年 | 2篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1997年 | 1篇 |
1993年 | 1篇 |
1991年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
排序方式: 共有300条查询结果,搜索用时 15 毫秒
141.
142.
Nasrin Samadi Neda Abadian Reza Ahmadkhaniha Farzaneh Amini Dina Dalili Noushin Rastkari Eliyeh Safaripour Farzaneh Aziz Mohseni 《Folia microbiologica》2012,57(6):501-508
The aim of present work was to study chemical structures and biological activities of rhamnolipid biosurfactants produced by Pseudomonas aeruginosa MN1 isolated from oil-contaminated soil. The results of liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis revealed that total rhamnolipids (RLs) contained 16 rhamnolipid homologues. Di-lipid RLs containing C10-C10 moieties were by far the most predominant congeners among mono-rhamnose (53.29?%) and di-rhamnose (23.52?%) homologues. Mono-rhamnolipids form 68.35?% of the total congeners in the RLs. Two major fractions were revealed in the thin layer chromatogram of produced RLs which were then purified by column chromatography. The retardation factors (R f) of the two rhamnolipid purple spots were 0.71 for RL1 and 0.46 for RL2. LC-MS/MS analysis proved that RL1 was composed of mono-RLs and RL2 consisted of di-RLs. RL1 was more surface-active with the critical micelle concentration (CMC) value of 15?mg/L and the surface tension of 25 mN/m at CMC. The results of biological assay showed that RL1 is a more potent antibacterial agent than RL2. All methicillin-resistant Staphylococcus aureus (MRSA) strains were inhibited by RLs that were independent of their antibiotic susceptibility patterns. RLs remarkably enhanced the activity of oxacillin against MRSA strains and lowered the minimum inhibitory concentrations of oxacillin to the range of 3.12?C6.25???g/mL. 相似文献
143.
Fred M. Kaplan Curtis H. Kugel III Neda Dadpey Yongping Shao Ethan V. Abel Andrew E. Aplin 《The Journal of biological chemistry》2012,287(50):41797-41807
ERK1/2 signaling is frequently dysregulated in tumors through BRAF mutation. Targeting mutant BRAF with vemurafenib frequently elicits therapeutic responses; however, durable effects are often limited by ERK1/2 pathway reactivation via poorly defined mechanisms. We generated mutant BRAFV600E melanoma cells that exhibit resistance to PLX4720, the tool compound for vemurafenib, that co-expressed mutant (Q61K) NRAS. In these BRAFV600E/NRASQ61K co-expressing cells, re-activation of the ERK1/2 pathway during PLX4720 treatment was dependent on NRAS. Expression of mutant NRAS in parental BRAFV600 cells was sufficient to by-pass PLX4720 effects on ERK1/2 signaling, entry into S phase and susceptibility to apoptosis in a manner dependent on the RAF binding site in NRAS. ERK1/2 activation in BRAFV600E/NRASQ61K cells required CRAF only in the presence of PLX4720, indicating a switch in RAF isoform requirement. Both ERK1/2 activation and resistance to apoptosis of BRAFV600E/NRASQ61K cells in the presence of PLX4720 was modulated by SHOC-2/Sur-8 expression, a RAS-RAF scaffold protein. These data show that NRAS mutations confer resistance to RAF inhibitors in mutant BRAF cells and alter RAF isoform and scaffold molecule requirements to re-activate the ERK1/2 pathway. 相似文献
144.
Haghighat Neda Mohammadshahi Majid Shayanpour Shokouh Haghighizadeh Mohammad Hossein 《Probiotics and antimicrobial proteins》2020,12(1):144-151
Probiotics and Antimicrobial Proteins - The aim of this study was to investigate the effects of probiotic and synbiotic supplementation on serum inflammatory markers, endotoxin, and anti-HSP70 in... 相似文献
145.
146.
Rahimi Bahareh Panahi Mohammad Saraygord-Afshari Neda Taheri Neda Bilici Merve Jafari Davod Alizadeh Effat 《Molecular biology reports》2021,48(7):5607-5619
Molecular Biology Reports - Over the last decade, mesenchymal stem cells (MSCs) have been considered a suitable source for cell-based therapy, especially in regenerative medicine. First, the... 相似文献
147.
Hitoshi Murata Yuko Ota Muneyoshi Yamaguchi Akiyoshi Yamada Shinichiro Katahata Yuichiro Otsuka Katsuhiko Babasaki Hitoshi Neda 《Mycorrhiza》2013,23(6):447-461
“Matsutake” mushrooms are formed by several species of Tricholoma sect. Caligata distributed across the northern hemisphere. A phylogenetic analysis of matsutake based on virtually neutral mutations in DNA sequences resolved robust relationships among Tricholoma anatolicum, Tricholoma bakamatsutake, Tricholoma magnivelare, Tricholoma matsutake, and Tricholoma sp. from Mexico (=Tricholoma sp. Mex). However, relationships among these matsutake and other species, such as Tricholoma caligatum and Tricholoma fulvocastaneum, were ambiguous. We, therefore, analyzed genomic copy numbers of σ marY1 , marY1, and marY2N retrotransposons by comparing them with the single-copy mobile DNA megB1 using real-time polymerase chain reaction (PCR) to clarify matsutake phylogeny. We also examined types of megB1-associated domains, composed of a number of poly (A) and poly (T) reminiscent of RNA-derived DNA elements among these species. Both datasets resolved two distinct groups, one composed of T. bakamatsutake, T. fulvocastaneum, and T. caligatum that could have diverged earlier and the other comprising T. magnivelare, Tricholoma sp. Mex, T. anatolicum, and T. matsutake that could have evolved later. In the first group, T. caligatum was the closest to the second group, followed by T. fulvocastaneum and T. bakamatsutake. Within the second group, T. magnivelare was clearly differentiated from the other species. The data suggest that matsutake underwent substantial evolution between the first group, mostly composed of Fagaceae symbionts, and the second group, comprised only of Pinaceae symbionts, but diverged little within each groups. Mobile DNA markers could be useful in resolving difficult phylogenies due to, for example, closely spaced speciation events. 相似文献
148.
149.
Tang Y Holbert MA Delgoshaie N Wurtele H Guillemette B Meeth K Yuan H Drogaris P Lee EH Durette C Thibault P Verreault A Cole PA Marmorstein R 《Structure (London, England : 1993)》2011,19(2):221-231
Yeast Rtt109 promotes nucleosome assembly and genome stability by acetylating K9, K27, and K56 of histone H3 through interaction with either of two distinct histone chaperones, Vps75 or Asf1. We report the crystal structure of an Rtt109-AcCoA/Vps75 complex revealing an elongated Vps75 homodimer bound to two globular Rtt109 molecules to form a symmetrical holoenzyme with a ~12?? diameter central hole. Vps75 and Rtt109 residues that mediate complex formation in the crystals are also important for Rtt109-Vps75 interaction and H3K9/K27 acetylation both in?vitro and in yeast cells. The same Rtt109 residues do not participate in Asf1-mediated Rtt109 acetylation in?vitro or H3K56 acetylation in yeast cells, demonstrating that Asf1 and Vps75 dictate Rtt109 substrate specificity through distinct mechanisms. These studies also suggest that Vps75 binding stimulates Rtt109 catalytic activity by appropriately presenting the H3-H4 substrate within the central cavity of the holoenzyme to promote H3K9/K27 acetylation of new histones before deposition. 相似文献
150.
Jalalvand Neda Esmaeili Davoud Bashi Moha mmad Mehdi Moghani Raiszadeh Mohammad Naeimi Sirous 《International journal of peptide research and therapeutics》2021,27(2):1167-1175
International Journal of Peptide Research and Therapeutics - Gastric cancer treatment remains a major challenge. There are many reports on the positive efficacy of Bacteriocins associated with... 相似文献