全文获取类型
收费全文 | 14349篇 |
免费 | 1169篇 |
国内免费 | 12篇 |
出版年
2023年 | 69篇 |
2022年 | 183篇 |
2021年 | 371篇 |
2020年 | 193篇 |
2019年 | 238篇 |
2018年 | 313篇 |
2017年 | 254篇 |
2016年 | 412篇 |
2015年 | 633篇 |
2014年 | 710篇 |
2013年 | 841篇 |
2012年 | 1159篇 |
2011年 | 1156篇 |
2010年 | 726篇 |
2009年 | 669篇 |
2008年 | 925篇 |
2007年 | 907篇 |
2006年 | 895篇 |
2005年 | 769篇 |
2004年 | 733篇 |
2003年 | 711篇 |
2002年 | 630篇 |
2001年 | 183篇 |
2000年 | 111篇 |
1999年 | 174篇 |
1998年 | 173篇 |
1997年 | 119篇 |
1996年 | 116篇 |
1995年 | 73篇 |
1994年 | 85篇 |
1993年 | 82篇 |
1992年 | 60篇 |
1991年 | 62篇 |
1990年 | 54篇 |
1989年 | 61篇 |
1988年 | 53篇 |
1987年 | 36篇 |
1986年 | 39篇 |
1985年 | 65篇 |
1984年 | 61篇 |
1983年 | 43篇 |
1982年 | 30篇 |
1981年 | 39篇 |
1980年 | 31篇 |
1979年 | 29篇 |
1978年 | 22篇 |
1977年 | 31篇 |
1976年 | 19篇 |
1974年 | 20篇 |
1969年 | 13篇 |
排序方式: 共有10000条查询结果,搜索用时 156 毫秒
41.
Nutritional conditional mutants of Drosophila melanogaster 总被引:1,自引:0,他引:1
42.
43.
44.
45.
Abalone are semimobile marine gastropods that form the basis of Australia's second most valuable fishery. A site off the coast of Port Arthur, Tasmania, was visited on six occasions. On each occasion, any unmarked live abalone found were marked with a unique identification number and were recorded. Any previously marked abalone found had its identification number and whether or not it was still alive recorded. This results in integrated mark-recapture-recovery data, as in Catchpole et al. (1998, Biometrics 54, 33-46). During the study period, abalone grew in size, and we model the survival of individuals as a function of their size, estimated from a fitted growth curve. The shells of dead animals are long lasting, and we extend existing methodology to allow for the possibility that an animal found dead may have been dead but overlooked for several visits. 相似文献
46.
I have been the Executive Editor of the Journal of PlanktonResearch (JPR) for three years (even if it feels like longer)but from January 2008 the baton is passed over to Roger Harris. My association with JPR started in earnest in the early 1990's;I was doing so much reviewing for the Journal that David Cushing,the then Editor (and, of 相似文献
47.
ABSTRACT Brood parasites often must overcome host defenses that may include behaviors that serve other functions, such as deterrence of predators and nest attendance during laying and incubation. Host use by brood parasites may also be influenced by competitors in areas where more than one parasitic species occurs. We identified the degree to which behavior of potential hosts and potential competitors affected laying by Brown‐headed Cowbirds (Molothrus ater) and Bronzed Cowbirds (M. aeneus) at a site in south Texas where they co‐occur. We watched potential host nests during the presunrise period to record cowbird laying and document nest visitation, laying, cowbird‐host encounters, and nest attentiveness by hosts. Hosts were frequently at their nests when cowbirds laid eggs (83% of 121 watches among nests of five host species) and cowbirds regularly encountered hosts (43–74% and 40–77% of watches per species of host for Brown‐headed and Bronzed cowbirds, respectively). Host nest defense infrequently interfered with cowbird laying and cowbirds rarely interacted with one another during laying. Overall, 12% of the 42 cowbird laying attempts that elicited host nest defense failed, resulting in cowbird eggs either laid atop hosts as they sat in nests or laid outside the nest cup. We clearly documented that relatively small hosts can thwart parasitism by cowbirds. Thus, the potential for successful defense of nests should be considered when assessing the evolution of behaviors to deter the removal of host eggs by cowbirds and mechanisms leading to nest abandonment. Regarding the latter, the presence of a cowbird at a nest would be a poor indicator for parasitism as some laying attempts were thwarted and unparasitized broods were reared at those nests. Despite the potential for nest defense to affect host use by cowbirds, we did not detect an effect of nest defense. Because most host defense was ineffective, we examined hypotheses for the timing of cowbird laying and host nest attendance. Our analysis of time of day of laying by Brown‐headed Cowbirds at our site and data compiled from the literature suggests that laying time is best predicted by the time of civil twilight (first light) rather than sunrise. 相似文献
48.
Summary The plasmid pKBT1 was derived by in vivo recE4-independent recombinational event(s) yielding a structure containing regions of plasmid and chromosomal origin. BamHI digests of plasmid pUB110 (Kanr/Neor) and Bg/II digests of pTL12 (Tmpr, leuA) were mixed, ligated and used to transform competent cells of a recE4 strain of Bacillus subtilis. Kanamycin-resistant transformants were electrophoretically screened for hybrid plasmids. Plasmid pKBT1 (8.0 kb) was smaller than pTL12 (10.4 kb) but larger than monomeric pUB110 (4.5 kb). Plasmid PKBT1 was stably maintained in recE4 strains of B. subtilis and conferred kanamycin resistance but did not specify trimethoprim resistance or leucine prototrophy. At least 86% of the pUB110 monomer length was present in pKBT1 and was completely contained within a single 5.58 kb HindIII fragment. The other segment of pKBT1 was of chromosomal origin as evidenced by lack of homology to pTL12 and strong hybridization to B. subtilis chromosomal DNA. At least one of the in vivo recE4-independent event(s) which produced pKBT1 must have involved intermolecular recombination between transforming and chromosomal DNA. This finding differs from previous reports in which recE4-independent recombination involving pUB110 sequences was a strictly intramolecular event. 相似文献
49.
The influence of diet on the growth of Stenonema vicarium (Walker) (Ephemeroptera: Heptageniidae) 总被引:1,自引:1,他引:0
Laboratory studies compared the growth rate of Stenonema vicarium (Walker) nymphs on diets of detritus and natural stream periphyton. In three consecutive runs of the experiment, growth rates
were consistently higher on periphyton (mean growth rate = 2.1% wet wt. d−1) than detritus (mean = 1.8% wet wt. d−1). The starting date of each run also significantly influenced growth rates. In each treatment growth rates generally decreased
over the course of the 3 runs, and ca. one-half of the nymphs in the last run did not molt or grow. It appeared that growth
of S. vicarium may be partially controlled by seasonal factors. 相似文献
50.
Freeze-Thawing of Aquaspirillum magnetotacticum Cells Selectively Releases Periplasmic Proteins 总被引:1,自引:1,他引:0 下载免费PDF全文
Lawrence C. Paoletti Kevin A. Short Nancy Blakemore Richard P. Blakemore 《Applied microbiology》1987,53(10):2590-2592
Cells of the gram-negative bacterium Aquaspirillum magnetotacticum, when suspended in buffer and freeze-thawed, produced pinkish orange supernatant fluid. The fluid contained ≤2.0% of total extractable outer membrane component 2-keto-3-deoxyoctonate or of the cytoplasmic membrane marker succinic dehydrogenase. Electrophoretic banding patterns and difference spectra of proteins and hemoproteins released by freeze-thawing cells were distinct from those of membrane-associated substances and similar to those of periplasmic substances obtained by applying conventional fractionation methods to this organism. 相似文献