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61.
Kiyotaka Akiyama Hiroyuki Morita Shiro Suetsugu Seiko Kuraba Yasuharu Numata Yoshihisa Yamamoto Kiyoko Inui Terukuni Ideura Noriko Wakisaka Kiyoko Nakano Hiroaki Oniki Tadaomi Takenawa Mutsushi Matsuyama Ashio Yoshimura 《Mammalian genome》2008,19(1):41-50
The BUF/Mna strain of rat is a model of focal and segmental glomerulosclerosis (FSGS) in which a quantitative trait locus
(QTL) for proteinuria, Pur1, has been identified. The aim of the present study was to identify candidates for the Pur1 gene. To narrow the Pur1 QTL, we performed fine QTL mapping and single nucleotide polymorphism (SNP) genotyping. To identify candidate genes, sequencing
and gene-expression analyses of all genes contained in the narrowed locus were conducted. The narrowed Pur1 region contained 25 genes. Among these genes, only the Arp3 gene was mutated in the BUF/Mna strain; it contained a missense mutation that caused an L111F substitution. This leucine is conserved across species. Gene-expression analysis failed to identify any other candidate genes
for Pur1. Arp3-mediated actin assembly abnormalities were visible in immunohistochemical and electron microscopic examinations of
podocytes in old BUF/Mna rats. Taken together, these data suggest that Arp3 is a candidate for the Pur1 gene. This observation is consistent with our growing recognition that abnormal signaling-induced assembly of actin in podocytes
leads to the development of FSGS.
Nucleotide sequence data reported in this article are available in the DDBJ/EMBL/GenBank database under accession numbers
AB292042-292043 and AB294577-294580. 相似文献
62.
Seiko Nakagawa 《Free radical research》2017,51(7-8):749-754
UV-photolysis of hydrogen peroxide is a useful technique to produce hydroxyl radical. However, it is not an appropriate method to estimate the reactivity of polyphenols with hydroxyl radicals because many of the polyphenol derivatives also absorb the UV-light to generate hydroxyl radicals. In this study, X-ray irradiation of water with a flowing system was applied to estimate the reactivity of hydroxyl radicals with polyphenols using electron spin resonance (ESR) spin trap. The obtained relative reaction rates reasonably agreed with previous data by pulse radiolysis. This method will be a useful technique to estimate the reactivity of antioxidants including polyphenols with hydroxyl radicals. 相似文献
63.
Shimizu K Udono T Tanaka C Narushima E Yoshihara M Takeda M Tanahashi A van Elsackar L Hayashi M Takenaka O 《Primates; journal of primatology》2003,44(2):183-190
Urinary estrone conjugates (E1C), pregnanediol-3-glucuronide (PdG), and follicle-stimulating hormone (FSH) were determined by enzyme immunoassays (EIAs)
during the normal menstrual cycle in the orangutan, gorilla, chimpanzee, and bonobo. Furthermore, the data were compared to
those levels in the human and long-tailed macaque. The results showed a typical preovulatory E1C surge and postovulatory increase in PdG in all species. The pattern of E1C during the menstrual cycle in the great apes more closely resembled the human than do the long-tailed macaque. A major difference
of E1C pattern between these species appeared in the luteal phase. In the great apes and the human, E1C exhibited two peaks, the first peak detected at approximately mid cycle and the second peak detected during the luteal phase.
On the other hand, in the long-tailed macaque, increase of E1C in the luteal phase was small or nonexistent. The gorilla, chimpanzee, and bonobo exhibited similar PdG trends. The orangutan
excreted one tenth less PdG than these species during the luteal phase. The long-tailed macaque also excreted low levels of
PdG. The patterns of FSH in orangutan, chimpanzee, bonobo and long-tailed macaque showed a marked mid-cycle rise and an early
follicular phase rise, similar to those in the human. Comparing similar taxa, a large difference was found in FSH of gorilla;
there were three peaks during the menstrual cycle. Thus, there is considerable species variation in the excretion of these
hormones during the menstrual cycle and comparative studies could be approached with a single method. The methods and baseline
data presented here provide the basis for a practical approach to evaluation and monitoring of ovarian events in the female
great apes.
Electronic Publication 相似文献
64.
Hamaguchi K Kuwata H Yoshihara K Masuda S Shimbara S Oh-ishi S Murakami M Kudo I 《Biochimica et biophysica acta》2003,1635(1):37-47
Although the expression of the prototypic secretory phospholipase A(2) (sPLA(2)), group IIA (sPLA(2)-IIA), is known to be up-regulated during inflammation, it remains uncertain if other sPLA(2) enzymes display similar or distinct profiles of induction under pathological conditions. In this study, we investigated the expression of several sPLA(2)s in rodent inflammation models. In lipopolysaccharide (LPS)-treated mice, the expression of sPLA(2)-V, and to a lesser extent that of sPLA(2)-IID, -IIE, and -IIF, were increased, whereas that of sPLA(2)-X was rather constant, in distinct tissues. 12-O-Tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema, in which the expression of sPLA(2)-IID, -IIF and -V was increased, was significantly reduced by YM-26734, a competitive sPLA(2)-IIA inhibitor that turned out to inhibit sPLA(2)-IID, -IIE, -V and -X as well. In contrast, sPLA(2)-IIA was dominant in carageenin-induced pleurisy in rats, where the accumulation of exudate fluids and leukocytes was significantly ameliorated by YM-26734. These results indicate that distinct sPLA(2)s can participate in inflammatory diseases according to tissues, animal species, and types of inflammation. 相似文献
65.
Four new des-A-ergostane derivatives including blazeispirols B, C, E and F were isolated from the cultured mycelia of fungus Agaricus blazei Murill and were established to be (20S, 22R, 23R, 24S)-14beta,22: 22,25-diepoxy-5-methoxy-des-A-ergosta-5,7,9,11-tetraen-23-ol; (20S, 22S, 23R, 24S)-14beta,22: 22,25-diepoxy-5-methoxy-des-A-ergosta-5,7,9-trien-23-ol; (20S, 22S, 23R, 24S)-14beta, 22: 22, 25-diepoxy-5-methoxy-des-A-ergosta-5,7,9,11-tetraene-19,23-diol and (20S, 22S, 23R, 24S)-14beta,22: 22,25-diepoxy-des-A-ergosta-5,7,9-triene-5,23-diol by comparison of extensive 1D and 2D NMR spectral data with that of blazeispirol A. 相似文献
66.
Norihide Yokoi Seiko Shimizu Kotaro Ishibashi Kazuhiro Kitada Hiroyuki Iwama Misako Namae Moriyuki Sugawara Tadao Serikawa Kajuro Komeda 《Mammalian genome》2000,11(2):111-114
We have previously described a rat autosomal recessive mutation, creeping (cre), causing severe ataxia and disarrangement of neuronal cells in the central nervous system. The mutant strain has recently
been successfully inbred, named Komeda Zucker creeping (KZC) rat. In the present study, we have performed a genetic analysis
of the creeping mutation, and mapped it to rat Chromosome (Chr) 4. Comparative mapping, together with the similarity of the
phenotype, suggested that the creeping mutation is homologous to the mouse reeler mutation. In fact, reelin expression was
markedly reduced in the homozygous mutant (cre/cre) animals compared with the normal littermates. Thus, the KZC rat should become a useful biological model with a novel mutation
in the reelin gene.
Received: 25 June 1999 / Accepted: 19 October 1999 相似文献
67.
Hirotaka Matsuo Tappei Takada Akiyoshi Nakayama Toru Shimizu Masayuki Sakiyama Seiko Shimizu 《Nucleosides, nucleotides & nucleic acids》2014,33(4-6):266-274
ATP-binding cassette transporter, sub-family G, member 2 (ABCG2/BCRP) is identified as a high-capacity urate exporter, and its dysfunction has an association with serum uric acid levels and gout/hyperuricemia risk. Generally, hyperuricemia has been classified into urate “overproduction type,” “underexcretion type,” and “combined type” based on only renal urate excretion, without considering an extra-renal pathway such as gut excretion. In this study, we investigated the effects of ABCG2 dysfunction on human urate handling and the mechanism of hyperuricemia.Clinical parameters for urate handling including urinary urate excretion (UUE) were examined in 644 Japanese male outpatients with hyperuricemia. The severity of their ABCG2 dysfunction was estimated by genotype combination of two common ABCG2 variants, nonfunctional Q126X (rs72552713) and half-functional Q141K (rs2231142).Contrary to the general understanding that ABCG2 dysfunction leads to decreased renal urate excretion, UUE was significantly increased by ABCG2 dysfunction (P = 3.60 × 10?10). Mild, moderate, and severe ABCG2 dysfunctions significantly raised the risk of “overproduction” hyperuricemia including overproduction type and combined type, conferring risk ratios of 1.36, 1.66, and 2.35, respectively.The present results suggest that common dysfunctional variants of ABCG2 decrease extra-renal urate excretion including gut excretion and cause hyperuricemia. Thus, “overproduction type” in the current concept of hyperuricemia should be renamed “renal overload type,” which is caused by two different mechanisms, “extra-renal urate underexcretion” and genuine “urate overproduction.”Our new concept will lead to a more accurate diagnosis and more effective therapeutic strategy for hyperuricemia and gout. 相似文献
68.
Ifuku K Nakatsu T Shimamoto R Yamamoto Y Ishihara S Kato H Sato F 《Photosynthesis research》2005,84(1-3):251-255
PsbP is a membrane extrinsic subunit of Photosystem II (PS II), which is involved in retaining Ca2+ and Cl−, two inorganic cofactors for the water-splitting reaction. In this study, we re-investigated the role of N-terminal region of PsbP on the basis of its three-dimensional structure. In previous paper [Ifuku and Sato (2002) Plant Cell Physiol 43: 1244–1249], a truncated PsbP lacking 19 N-terminal residues (Δ19) was found to bind to NaCl-washed PS II lacking PsbP and PsbQ without activation of oxygen evolution at all. Three-dimensional (3D) structure of PsbP suggests that deletion of 19 N-terminal residues would destabilize its protein structure, as indicated by the high sensitivity of Δ19 to trypsin digestion. Thus, a truncated PsbP lacking 15 N-terminal residues (Δ15), which retained core PsbP structure, was produced. Whereas Δ15 was resistant to trypsin digestion and bound to NaCl-washed PS II membranes, it did not show the activation of oxygen evolution. This result indicated that the interaction of 15-residue N-terminal flexible region of PsbP with PS II was important for Ca2+ and Cl− retention in PS II, although the 15 N-terminal residues were not essential for the binding of PsbP to PS II. The possible N-terminal residues of PsbP that would be involved in this interaction are discussed. 相似文献
69.
Tsutomu Ikeda Youichi Takanami Seiko Imaizumi Takashi Matsumoto Youichi Mikami Susumu Kubo 《Plant cell reports》1987,6(3):216-218
The extract of Mirabilis jalapa cultured cells and its precipitate fraction with 90% saturated ammonium sulfate showed an anti-plant viral activity comparable to that of the roots and leaves of the original plant. In the immunodiffusion experiment, the extract of cultured cells positively reacted with MAP (Mirabilis Anti-plant viral Protein) anti-serum. The changes in MAP formation during cell growth and the MAP content of roots and leaves were examined using enzyme-linked immunosorbent assay (ELISA). MAP formation proceeded almost in parallel with cell growth. The MAP content of cultured cells reached the highest level (0.6 mg/g dry weight) on the 9th day after inoculation, which was less than one-third of the content of the roots but three times larger than that of the leaves.Abbreviations MAP
Mirabilis anti-plant viral protein
- TMV
tobacco mosaic virus
- 2,4-D
2,4-dichlorophenoxyacetic acid
- ELISA
enzyme-linked immunosorbent assay
Studies on the production of anti-plant viral substances of higher plant cells in suspension culture. Part 1 相似文献