全文获取类型
收费全文 | 1163篇 |
免费 | 82篇 |
国内免费 | 6篇 |
出版年
2023年 | 9篇 |
2022年 | 15篇 |
2021年 | 32篇 |
2020年 | 10篇 |
2019年 | 22篇 |
2018年 | 30篇 |
2017年 | 25篇 |
2016年 | 25篇 |
2015年 | 40篇 |
2014年 | 61篇 |
2013年 | 68篇 |
2012年 | 71篇 |
2011年 | 82篇 |
2010年 | 56篇 |
2009年 | 53篇 |
2008年 | 63篇 |
2007年 | 75篇 |
2006年 | 52篇 |
2005年 | 41篇 |
2004年 | 49篇 |
2003年 | 49篇 |
2002年 | 33篇 |
2001年 | 18篇 |
2000年 | 25篇 |
1999年 | 16篇 |
1998年 | 16篇 |
1997年 | 10篇 |
1996年 | 10篇 |
1995年 | 9篇 |
1994年 | 11篇 |
1993年 | 5篇 |
1992年 | 12篇 |
1991年 | 10篇 |
1989年 | 10篇 |
1988年 | 11篇 |
1987年 | 5篇 |
1986年 | 5篇 |
1985年 | 16篇 |
1984年 | 8篇 |
1982年 | 8篇 |
1980年 | 8篇 |
1979年 | 8篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1976年 | 7篇 |
1975年 | 5篇 |
1974年 | 7篇 |
1972年 | 5篇 |
1967年 | 5篇 |
1966年 | 5篇 |
排序方式: 共有1251条查询结果,搜索用时 234 毫秒
21.
Thirty-nine enterotoxigenic cultures of Clostridium perfringens type A were studied for enterotoxin and haemagglutinin production. Enterotoxin was quantitated by sandwich ELISA and DOT-ELISA techniques and haemagglutinin titres were determined using sheep and human erythrocytes. Haemagglutinins from only six cultures reacted against both sheep and human erythrocytes; a further 13 reacted only against human erythrocytes, and another five only against sheep cells.The authors are with the Department of Veterinary Public Health and Epidemiology, Ranchi Veterinary College, Birsa Agricultural University, Ranchi-834007 (Bihar), India. 相似文献
22.
Buoyant density gradient analysis of nuclear DNA of fourCucumis species showed asymmetric profiles indicating the presence of satellite DNA sequences in the nuclear genome. A highly repeated satellite DNA sequence was isolated from the nuclear genome ofC. metuliferus under neutral CsCl gradients. The satellite DNA constitutes about 4.96% of total nuclear DNA and has 48.06% guanine plus cytosine content. The kinetic complexity of satellite DNA is 150 times smaller than T4 phage DNA and the base sequence divergence is low.3H-labeled cRNA transcribed from satellite DNA hybridized clearly to six heterochromatic knobs of pachytene chromosomes. The knob heterochromatin can be distinguished by Giemsa C-banding of pachytene chromosomes. Restriction enzyme analysis and Southern blot hybridization indicated that the satellite DNA has a tandem arrangement and predominantly formed two bands of size 210 and 151 base pairs. Absence of knob satellite DNA ofC. metuliferus in the nuclear genomes ofC. melo, C. anguria andC. sativus showed thatC. metuliferus remains isolated within the genusCucumis. 相似文献
23.
Summary Nutritional requirements for the production of ergot alkaloids were studied with Aspergillus fumigatus under submerged conditions of fermentation, in a chemically defined medium. Glucose in combination with mannitol and triammonium citrate were found to be the best sources of carbon and nitrogen for the production of alkaloids. Carbon to nitrogen ratio of 4.16 : 1 was found optimum. Phosphate at elevated concentration inhibited alkaloid production. 相似文献
24.
25.
26.
Kapoor V Paliwal D Baskar Singh S Mohanti BK Das SN 《Biochemical and biophysical research communications》2012,422(4):764-769
Autophagy is a physiologically regulated and evolutionary conserved process that plays a critical role in degradation of cytoplasmic proteins and other macromolecules within the lysosomes. Beclin-1, the mammalian orthologue of yeast Atg6, is an important mediator of autophagy that has been studied in many human cancers. However, the expression of Beclin-1 has not yet been investigated in oral cancer. We for the first time investigated the expression of Beclin-1 in serum and tissues and correlated it with the clinic-pathological features of oral cancer patients. m-RNA expression of Beclin-1 was evaluated in tumor and normal areas of surgical specimens from 10 oral cancer patients by real-time PCR. Approximately, 8-fold lower expression (p<0.001) of Beclin-1 mRNA was observed in tumor tissue as compared to the normal tissue. Serum levels of Beclin-1 were evaluated by SPR and ELISA. No significant difference was observed in serum Beclin-1 levels in patients as compared to healthy subjects, similarly no correlation was found between serum levels and clinic-pathological parameters such as stage, lymph node involvement and tumor size. Our results demonstrate that down-regulation of Beclin-1 may play an important role in the development and progression of oral cancer possibly by dysregulation of autophagy in tumor cells. 相似文献
27.
B R Srinivasa 《Biochemistry international》1984,9(4):523-529
The reduction of insulin by tri-n-butylphosphine followed by air oxidation in dilute solution at pH 9.1 yields A- and B-chain disulfides. A(S-S)2 and B(S-S) have been purified on SP-Sephadex C-25 using a linear gradient of sodium chloride from 0.1 to 0.45 M in 0.5 M acetic acid containing 7 M urea. The overall yield of A(S-S)2 was 70%; and B(S-S), 60%. The A(S-S)2 and B(S-S) had the expected amino acid composition and N-terminal amino acid. The kinetics of reduction and reoxidation of insulin disulfide bonds are discussed. 相似文献
28.
29.
Narasimsetti Srinivasa Rao Megala Rajesh Kumcham Prasad Gutturu Rajasekhara Reddy B. Deva Prasad Raju S. Dhanapandian 《Luminescence》2020,35(5):721-727
Sm3+ ions doped strontium lithium lead borate glasses (SLLB:Sm) were prepared using a conventional melt‐quenching technique. The glasses were analyzed using X‐ray diffractometry and Fourier transform infrared spectroscopy, optical absorption, fluorescence spectral analysis, and fluorescence lifetime decay. The Judd–Ofelt (J–O) parameters and radiative parameters of the SLLB:Sm10 glass (1.0 mol% Sm3+ ion‐doped glass) were calculated using J–O theory. From the emission spectra, among all the synthesized glass, SLLB:Sm10 glass had the highest emission intensity for 4G5/2→6H11/2 transition (610 nm). Emission parameters, such as stimulated emission cross‐section and optical gain bandwidth, were calculated. For all concentrations of Sm3+ ions, the decay profile showed an exponential nature and decreased when the Sm3+ ion concentration was increased due to a concentration quenching effect. This result suggests that the synthesized SLLB:Sm10 glass could be used for application in high‐density optical memory devices. 相似文献
30.
Mohanan Manoj Vadakkenchery Pushpanathan Anunanthini Padmanabhan Sarath Sasikumar Thelakat Jayanarayanan Ashwin Narayan Selvarajan Dharshini Ramalingam Sathishkumar Ram Bakshi Chinnaswamy Appunu 《Journal of plant research》2021,134(5):1083-1094
Journal of Plant Research - The glyoxalase pathway is a check point to monitor the elevation of methylglyoxal (MG) level in plants and is mediated by glyoxalase I (Gly I) and glyoxalase II (Gly II)... 相似文献