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121.
Masahiro Uchida Shuji Hanai Naoya Uematsu Kazunobu Sawamoto Hideyuki Okano Masanao Miwa Kazuhiko Uchida 《The Journal of biological chemistry》2002,277(8):6696-6702
Poly(ADP-ribose) polymerase (PARP) may play important roles in nuclear events such as cell cycle, cell proliferation, and maintenance of chromosomal stability. However, the exact biological role played by PARP or how PARP is involved in these cellular functions is still unclear. To elucidate the biological functions of PARP in vivo, we have constructed transgenic flies that overexpress Drosophila PARP in the developing eye primordia. These flies showed mild roughening of the normally smooth ommatidial lattice and tissue polarity disruption caused by improper rotation and chirality of the ommatidia. To clarify how this phenotypical change was induced, here we analyzed transgenic flies overexpressing PARP in the developing eye, embryo, and adult in detail. PARP mRNA level and the phenotype were enhanced in flies carrying more copies of the transgene. Developing eyes from third instar larvae were analyzed by using the neural cell marker to examine the involvement of PARP in cell fate. Morphological disorder of non-neuronal accessory cells was observed in PARP transgenic flies. Interestingly, overexpression of PARP did not interfere with the cell cycle or apoptosis, but it did disrupt the organization of cytoskeletal F-actin, resulting in aberrant cell and tissue morphology. Furthermore, heat-induced PARP expression disrupted organization of cytoskeletal F-actin in embryos and tissue polarity in adult flies. Because these phenotypes closely resembled mutants or transgenic flies of the tissue polarity genes, genetic interaction of PARP with known tissue polarity genes was examined. Transgenic flies expressing either PARP or RhoA GTPase in the eye were crossed, and co-expression of PARP suppressed the effect of RhoA GTPase. Our results indicate that PARP may play a role in cytoskeletal or cytoplasmic events in developmental processes of Drosophila. 相似文献
122.
Does the photosynthetic light‐acclimation need change in leaf anatomy? 总被引:23,自引:3,他引:20
There is a strong correlation between leaf thickness and the light‐saturated rate of photosynthesis per unit leaf area (Pmax). However, when leaves are exposed to higher light intensities after maturation, Pmax often increases without increasing leaf thickness. To elucidate the mechanism with which mature leaves increase Pmax, the change in anatomical and physiological characteristics of mature leaves of Chenopodium album, which was transferred from low to high light condition, were examined. When compared with leaves subjected to low light continuously (LL leaves), the leaves transferred from low to high light (LH leaves) significantly increased Pmax. The transfer also increased the area of chloroplasts facing the intercellular space (Sc) and maintained a strong correlation between Pmax and Sc. The mesophyll cells of LL leaves had open spaces along cell walls where chloroplasts were absent, which enabled the leaves to increase Pmax when they were exposed to high light (LH). However, the LH leaves were not thick enough to allow further increase in Pmax to the level in HH leaves. Thus leaf thickness determines an upper limit of Pmax of leaves subjected to a change from low to high light conditions. Shade leaves would only increase Pmax when they have open space to accommodate chloroplasts which elongate after light conditions improve. 相似文献
123.
Naoya YASHIRO Yoshimi HIROSE Jeffrey Y. HONDA Yoshinori TAKEUCHI Toshihisa YASHIRO 《Entomological Science》2012,15(2):189-196
A new species of Trichogramma that parasitizes Sialis melania eggs is described as Trichogramma tajimaense Yashiro, Hirose and Honda, sp. nov. from Japan. Its phylogenetic position is based on a DNA‐based analysis, and data regarding its male wing polymorphism are also presented. The view that T. tajimaense is closely related to T. semblidis, another parasitoid of Sialis eggs, is supported by the results of a phylogenetic analysis, as well as by the biological and morphological similarities between both species. Trichogramma tajimaense is also similar in male wing polymorphism to T. kurosuae, a gregarious egg parasitoid of the lepidopteran Ivela auripes, as both Trichogramma species exhibit male wing trimorphism (fully alate, brachypterous and apterous forms) in contrast to the male wing dimorphism (fully alate and apterous forms) of T. semblidis. However, no phylogenetic analysis reveals a close relationship between T. tajimaense and T. kurosuae, and a difference exists between these two species in the mean percentage of flightless (brachypterous and apterous) males that emerge from a host egg mass; 96% of T. tajimaense males are incapable of flight, whereas about 50% of T. kurosuae males are flightless. Because all or almost all males of T. semblidis parasitizing Sialis eggs are apterous, T. tajimaense is more similar to T. semblidis than to T. kurosuae in the proportion of flightless males. In addition, male wing polymorphisms of Trichogramma in relation to mating systems could also show a similarity between T. tajimaense and T. semblidis when considering both species as quasi‐gregarious parasitoids of Sialis eggs. 相似文献
124.
Kyung-Mook Yun Hideki Morimoto Naoya Shibayama 《The Journal of biological chemistry》2002,277(3):1878-1883
Considerable controversy remains as to the functional and structural properties of the asymmetric alpha1beta1 half-oxygenated intermediate of human hemoglobin, consisting of a deoxygenated and an oxygenated dimer. A recent dimer-tetramer equilibrium study using [Zn(II)/Fe(II)-O(2)] hybrid hemoglobins, in which Zn-protoporphyrin IX mimics a deoxyheme, showed that the key intermediate, [alpha(Fe-O(2))beta(Fe-O(2))][alpha(Zn)beta(Zn)], exhibited an enhanced tetramer stability relative to the other doubly oxygenated species. This is one of the strongest findings in support of distinctly favorable intra-dimer cooperativity within the tetramer. However, we present here a different conclusion drawn from direct O(2) binding experiments for the same asymmetric hybrid, [alpha(Fe)beta(Fe)][alpha(Zn)beta(Zn)], and those for [alpha(Fe)beta(Zn)](2) and [alpha(Zn)beta(Fe)](2). In this study, the O(2) equilibrium curves for [alpha(Fe)beta(Fe)][alpha(Zn)beta(Zn)] were determined by an O(2)-jump stopped-flow technique to circumvent the problem of dimer rearrangement, and those for [alpha(Fe)beta(Zn)]( 2) and [alpha(Zn)beta(Fe)]( 2) were measured by using an Imai apparatus. It was shown that the first and second O(2) equilibrium constants for [alpha(Fe)beta(Fe)][alpha(Zn)beta(Zn)] are 0.0209 mmHg(-1) and 0.0276 mmHg(-1), respectively, that are almost identical to those for [alpha(Fe)beta(Zn)](2) or [alpha(Zn)beta(Fe)](2). Therefore, we did not observe large difference among the asymmetric and symmetric hybrids. The discrepancy between the present and previous studies is mainly due to previously observed negative cooperativity for [alpha(Fe)beta(Zn)](2) and [alpha(Zn)beta(Fe)](2), which is not the case in our direct O(2) binding study. 相似文献
125.
Seasonal changes in the reproductive and life‐history traits of Chrysomela populi L. were investigated in Shobara (34°52′N, 133°01′E), Japan, in 2004 and 2005. Larvae were hatched and reared under natural photoperiod and temperature in 8 different periods between late April and early September in 2004. The incidence of adult diapause increased with progression of the season in 2004. The critical timing of diapause induction, as defined by 50% of females entering diapause, was estimated to occur between late July and early August. No effects of season on the survival rate from hatching to adult emergence, percentage ovipositing females and percentage females that deposited viable eggs were clearly detected. Adult weight at emergence fluctuated seasonally, which was probably caused by air temperature. No effect of season on the oviposition period was observed among females that averted diapause. Females that entered diapause in 2004 started oviposition from the first day of May in 2005. Reproductive output was significantly greater in diapause females than in non‐diapause females. This increase in the reproductive output of diapause females was caused by elongation of the oviposition period and consequently by enlargement of the number of egg clutches deposited. These results suggest that the seasonal timing of diapause induction in females of C. populi would be affected by various risks and benefits to reproduction and survival. 相似文献
126.
Naoya Sata 《Parasitology international》2018,67(4):493-500
To clarify how the species diversity of highly dispersible parasites has developed, molecular phylogenetic analyses of Meteterakis spp., multi-host endoparasitic nematodes of reptiles and amphibians from the East Asian islands, were conducted. The results demonstrated the existence of two major clades, the J- and A-groups, with exclusive geographic ranges that are discordant with the host faunal province. However, diversification within the J-group was concordant with the host biogeography and suggested co-divergence of this group with vicariance of the host fauna. In contrast, the phylogenetic pattern within the A-group was discordant with host biogeography and implied diversification by repeated colonization. In addition, the mosaic distribution pattern of a J-group and an A-group species in the Japanese Archipelago, along with comparison of population genetic parameters and the genetic distance from their closest relatives, suggested the initial occurrence of a J-group lineage followed by exclusion in the western part of this region caused by invasion of an A-group lineage. Thus, the present study suggested that the species diversity of highly dispersible parasites including Meteterakis is formed not only by co-divergence with host faunal vicariance but also by peripatric speciation and exclusive interactions between species. 相似文献
127.
Katsuaki Yokoyama Shigemasa Tani Rei Matsuo Naoya Matsumoto 《BMC cardiovascular disorders》2018,18(1):224
Background
Lecithin-cholesterol acyltransferase (LCAT) is believed to be involved in reverse cholesterol transport, which is known to play a key role in suppression of atherosclerosis. However, recent investigations have demonstrated that higher LCAT activity, measured in terms of the serum cholesterol esterification rate by an endogenous substrate method, is associated with increased formation of triglyceride (TG)-rich lipoproteins (TRLs), leading to a decrease in the low-density lipoprotein (LDL) particle size. The purpose of this hospital-based longitudinal study was to clarify the causal relationship between changes in the LCAT activity and changes in the LDL-particle size.Methods
The subjects were a total of 335 patients, derived from our previous study cohort, with one or more risk factors for atherosclerotic cardiovascular disease (ASCVD). For this study, we measured the LDL-particle size (relative LDL migration [LDL-Rm value]) by polyacrylamide gel electrophoresis in the subjects, along with the changes in the LCAT activity, at the end of a follow-up period of at least 1 year.Results
The results revealed that the absolute change (Δ) in the LDL-particle size increased significantly as the quartile of Δ LCAT activity increased (p =?0.01). A multi-logistic regression adjusted-analysis revealed that Δ LCAT activity in the fourth quartile as compared to that in the first quartile was independently predictive of an increased LDL-particle size (odds ratio [95% confidence interval]: 2.03 [1.02/4.04], p =?0.04). Moreover, the ? LCAT activity was also positively correlated with ? TRL-related markers (i.e., TG, remnant particle-like cholesterol [RLP-C], apolipoprotein B, apolipoprotein C-2, and apolipoprotein C-3).Conclusions
The results lend support to the hypothesis that increased LCAT activity may be associated with increased formation of TRLs, leading to a reduction in the LDL-particle size in patients at a high risk for ASCVD. To reduce the risk of ASCVD, it may be important to focus not only on the quantitative changes in the serum LDL-cholesterol levels, but also on the LCAT activity.Trial registration
UMIN (https://upload.umin.ac.jp/cgi-bin/ctr/ctr_reg_list.cgi) Study ID: UMIN000033228 retrospectively registered 2 July 2018.128.
Efficient Solar‐Driven Water Oxidation over Perovskite‐Type BaNbO2N Photoanodes Absorbing Visible Light up to 740 nm
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Takashi Hisatomi Mamiko Nakabayashi Naoya Shibata Tsutomu Minegishi Masao Katayama Kazunari Domen 《Liver Transplantation》2018,8(24)
Photoelectrochemical water splitting using semiconductors absorbing a wide range of visible light is a potentially attractive means of harvesting large portions of the solar spectrum. However, this is also very challenging because narrowing the semiconductor band gap lowers the driving force for photoreactions. Herein, a highly active perovskite BaNbO2N exhibiting photoexcitation up to 740 nm for water oxidation is reported. The synthesis route, consisting of moderate nitridation and subsequent annealing in inert Ar flow, enhances the crystallinity of the BaNbO2N surface without inducing the reduction of the Nb species. As a result, a particulate BaNbO2N photoanode exhibits a photocurrent of 5.2 mA cm?2 at 1.23 VRHE under simulated solar irradiation, which is the highest yet reported for an oxynitride responsive at wavelengths above 600 nm. Suppressing the reduction of B‐site cations during the synthesis of perovskite AB(O,N)3, which otherwise results in surface defects or impurities, is critical for achieving high water oxidation activity. 相似文献
129.
This work focused on the characteristics of ethanol regulation from Monascus sp. NP1. in glucose liquid medium, a saccharification method using algae and bioethanol production from Cladophora glomerata by the fungus. The results showed that when the fungus was grown in glucose (2, 20, 40 and 50%) medium under 110 rpm rotary culture at 30 °C, the ethanol concentration at 120 h increased from 2 to 20% glucose, where it peaked. It then decreased gradually to 40%, with production stopping at 50% glucose. This result indicated the glucose regulation of ethanol production by the fungus. Ethanol present in 20% glucose medium was identified by retention time and co-injection with a standard to demonstrate that the product was ethanol. Its yield was 285 mM [13 g L?1 or 65 mg (g of glucose substrate)?1] with a low interference of by-products. Three-millimetre-long pieces of dried algae were cut and exposed to concentrations of 1, 2, 3, 4, 5 and 6 g in 65 mL of 0.3 N hydrochloric acid or sulfuric acid before autoclaving (121 °C, 15 psi, 15 min). The amount of reducing sugar was greater than that of the control (without acid treatment) and varied with the increasing quantity of algae. The best condition was sulfuric acid and 6 g dried algae. The type of acid appeared to affect saccharification. During 12 days of fermentation in algal extraction (2 g reducing sugar per millilitre algal extraction), the mould could produce twofold more ethanol yield [34–55 mg (100 g dried weight algae)?1] than the yeast, Saccharomyces cerevisiae TISTR 5049. 相似文献
130.
Jianping Li Sadahiko Iwamoto Naoya Sugimoto Hiroshi Okuda E. Kajii 《Human genetics》1997,99(5):573-577
The Duffy blood group system consists of three alleles, FYA, FYB, and FY. To study the molecular evolution of the three alleles,
we established the polymorphism of a dinucleotide (GT) repeat sequence (designated FyGT/ C) in the 3′ flanking region of the
Duffy gene, and studied the relationship between FyGT/C and Duffy polymorphism in Japanese, people of African origin, and
chimpanzee. By single-strand conformation polymorphism and sequence analysis, five and two alleles were identified in Japanese
and Africans, respectively. In 110 random Japanese, the FyGT/C genotypes observed were in agreement with Hardy-Weinberg law.
From the sequence of the chimpanzee Duffy gene, including both flanking regions, FYB was identified as the ancestral gene
of the human alleles. The FyGT/C sequences associated with the FY allele of Africans were distinct from those of Duffy positives,
whereas the FYB and FYA alleles shared common FyGT/C sequences. Thus, it is suggested that the first split took place between
the FYB and FY alleles, and the second between the FYB and FYA alleles.
Received: 25 July 1996 / Revised: 10 October 1996 相似文献