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191.
192.
Mitsuhiro Wada Megumi Kira Hirotsugu Kido Rie Ikeda Naotaka Kuroda Toshiaki Nishigaki Kenichiro Nakashima 《Luminescence》2011,26(3):191-195
A semi‐micro flow injection analysis (SMFIA) method for evaluation of quenching effect of food additive antioxidants or health foods on peroxynitrite (ONOO‐) is described. The injected sample was carried with phosphate buffer containing NaNO2, mixed with a trigger solution to generate ONOO‐ and then detected CL generated after mixing with luminol solution. Selective chemiluminescence caused by ONOO‐ in this generation system was confirmed by catalase treatment. Ascorbic acid (ASA), Trolox and ascorbyl palmitate (ASP) were used as food additive antioxidants. EC50 values of ASA, Trolox and ASP were 2.6, 6.4 and 43 µg/mL, respectively. The amount of reagents required for an assay by this SMFIA system could reduce the time by a third compared with the conventional method previously reported. Furthermore, as an application of the proposed method, the quenching effect of commercially available Noni (Morinda citrifolia) juices was evaluated. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
193.
Tanita Frey Tomonari Murakami Koichiro Maki Takumi Kawaue Naoki Tani Ayaka Sugai Naotaka Nakazawa Kei-ichiro Ishiguro Taiji Adachi Mineko Kengaku Kenichi Ohki Yukiko Gotoh Yusuke Kishi 《Aging cell》2023,22(9):e13925
Neurons decline in their functionality over time, and age-related neuronal alterations are associated with phenotypes of neurodegenerative diseases. In nonneural tissues, an infolded nuclear shape has been proposed as a hallmark of aged cells and neurons with infolded nuclei have also been reported to be associated with neuronal activity. Here, we performed time-lapse imaging in the visual cortex of Nex-Cre;SUN1-GFP mice. Nuclear infolding was observed within 10 min of stimulation in young nuclei, while the aged nuclei were already infolded pre-stimulation and showed reduced dynamics of the morphology. In young nuclei, the depletion of the stimuli restored the nucleus to a spherical shape and reduced the dynamic behavior, suggesting that nuclear infolding is a reversible process. We also found the aged nucleus to be stiffer than the young one, further relating to the age-associated loss of nuclear shape dynamics. We reveal temporal changes in the nuclear shape upon external stimulation and observe that these morphological dynamics decrease with age. 相似文献
194.
195.
Taichi Usui Minoru Yokoyama Naotaka Yamaoka Kazuo Matsuda Katura Tuzimura Horoshi Sugiyama Shuichi Seto 《Carbohydrate research》1974,33(1):105-116
The p.m.r. spectra of some D-gluco-oligosaccharides and D-glucans in deuterium oxide were studied with respect to the anomeric proton. In (1→2)-linked glucobioses, the effect of change in configuration of the hydroxyl group at C-1 on the chemical shifts of the glycosidic proton is noted. Equilibrium mixtures of (1→2)-linked glucobioses contained more α-anomer than did the other examples, despite the cis configuration of substituents at C-1 and C-2. Some D-glucans were investigated with regard to the degree of branching, although solubility was a limitation. 相似文献