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51.
The structure of N i -( N '-Sulfodiaminophosphinyl)- l -ornithine (PSOrn) in complex with the enzyme ornithine transcarbamoylase (OTCase) was recently characterised by Langley et al. [D.B. Langley, M.D. Templeton, B.A. Fields, R.E. Mitchell and C.A. Collyer, J. Biol. Chem., 275 (2000) 20012] using X-ray diffraction techniques. In this work, the interaction of PSOrn with the arginine residues of OTCase is modelled using density functional theory, with an emphasis on characterising the mechanism of binding between PSOrn, an inhibitor, and the enzyme. For the purposes of this study, the interaction of PSO, an analogue of PSOrn (obtained by replacing a (CH 2 ) 3 CH( CO 2 m )( NH 3 + ) side chain by methyl) with one and two arginine (Arg) molecules are investigated. The PSO > (Arg) 2 trimer is found to be strongly bound, by ~171 kJ mol m 1 , due to the presence of four hydrogen bonds in addition to a large ionic interaction between a dinegative PSO 2 m and protonated arginines. The computed geometry is consistent with the X-ray structure and the large binding energy is consistent with the observation that PSOrn is a powerful inhibitor. Furthermore, in agreement with the proposals of Langley et al. , the most stable bound form of PSO is found to be an imino type tautomer. The population analyses that were carried out on PSO suggest that PN, PO, SN and SO bonds, as in a range of other systems, are generally either single or semipolar bonds. 相似文献
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Naomi B. Farber 《Ethnic and racial studies》2013,36(1):200-209
Bette J. Dickerson (ed.), AFRICAN AMERICAN SINGLE MOTHERS: UNDERSTANDING THEIR LIVES AND FAMILIES, Thousand Oaks, CA: Sage Publications, 1995, xxxii + 200 pp., $24.00 (paper). Linda Gordon, PITIED BUT NOT ENTITLED: SINGLE MOTHERS AND THE HISTORY OF WELFARE 1890–1935, Cambridge, MA: Harvard University Press, 1994, 433 pp., $15.95 (paper). Joyce A. Ladner, TOMORROW'S TOMORROW: THE BLACK WOMAN, Lincoln, NE: The University of Nebraska Press, 1995, xxiii + 304 pp., $12.00 (paper). Carl Husemoller Nightingale, ON THE EDGE: A HISTORY OF POOR BLACK CHILDREN AND THEIR AMERICAN DREAMS, New York: Basic Books, 1993, xv + 254 pp., $14.00 (paper). M. Belinda Tucker and Claudia Mitchell‐Kernan, (eds), THE DECLINE IN MARRIAGE AMONG AFRICAN AMERICANS: CAUSES, CONSEQUENCES AND POLICY IMPLICATIONS, New York: Russell Sage Foundation, 1995, xxiv + 397 pp., $49.95 and $19.95 (paper). 相似文献
54.
Robert H. C. Chen Sabine Wislet-Gendebien Filsy Samuel Naomi P. Visanji Gang Zhang Diana Marsilio Tammy Langman Paul E. Fraser Anurag Tandon 《The Journal of biological chemistry》2013,288(11):7438-7449
α-Synuclein is an abundant presynaptic protein and a primary component of Lewy bodies in Parkinson disease. Although its pathogenic role remains unclear, in healthy nerve terminals α-synuclein undergoes a cycle of membrane binding and dissociation. An α-synuclein binding assay was used to screen for vesicle proteins involved in α-synuclein membrane interactions and showed that antibodies directed to the Ras-related GTPase Rab3a and its chaperone RabGDI abrogated α-synuclein membrane binding. Biochemical analyses, including density gradient sedimentation and co-immunoprecipitation, suggested that α-synuclein interacts with membrane-associated GTP-bound Rab3a but not to cytosolic GDP-Rab3a. Accumulation of membrane-bound α-synuclein was induced by the expression of a GTPase-deficient Rab3a mutant, by a dominant-negative GDP dissociation inhibitor mutant unable to recycle Rab3a off membranes, and by Hsp90 inhibitors, radicicol and geldanamycin, which are known to inhibit Rab3a dissociation from membranes. Thus, all treatments that inhibited Rab3a recycling also increased α-synuclein sequestration on intracellular membranes. Our results suggest that membrane-bound GTP-Rab3a stabilizes α-synuclein on synaptic vesicles and that the GDP dissociation inhibitor·Hsp90 complex that controls Rab3a membrane dissociation also regulates α-synuclein dissociation during synaptic activity. 相似文献
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Sascha Naomi McKeon Marta Moreno Maria Anise Sallum Marinete Marins Povoa Jan Evelyn Conn 《Memórias do Instituto Oswaldo Cruz》2013,108(5):605-615
To evaluate whether environmental heterogeneity contributes to the
genetic heterogeneity in Anopheles triannulatus, larval habitat
characteristics across the Brazilian states of Roraima and Pará and genetic
sequences were examined. A comparison with Anopheles goeldii
was utilised to determine whether high genetic diversity was unique to
An. triannulatus. Student t test and
analysis of variance found no differences in habitat characteristics between the
species. Analysis of population structure of An. triannulatus
and An. goeldii revealed distinct demographic histories in a
largely overlapping geographic range. Cytochrome oxidase I
sequence parsimony networks found geographic clustering for both species;
however nuclear marker networks depicted An. triannulatus with
a more complex history of fragmentation, secondary contact and recent
divergence. Evidence of Pleistocene expansions suggests both species are more
likely to be genetically structured by geographic and ecological barriers than
demography. We hypothesise that niche partitioning is a driving force for
diversity, particularly in An. triannulatus. 相似文献
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59.
Selection of High Ubiquinone 10-Producing Strain of Tobacco Cultured Cells by Cell Cloning Technique
Takashi Matsumoto Tsutomu Ikeda Naomi Kanno Takuro Kisaki Masao Noguchi 《Bioscience, biotechnology, and biochemistry》2013,77(4):967-969
A novel enzyme, which was named Nα-benzyloxycarbonyl amino acid urethane hydrolase, was purified from a cell-free extract of Streptococcus faecalis R ATCC 8043, using Nα-benzyloxycarbonyl glycine as substrate. The enzyme was purified 1300-fold with an activity yield of 8%. The purified enzyme was homogeneous by disc electrophoresis. The molecular weight of the native enzyme is about 220,000 by gel filtration, and a molecular weight of 32,000 was determined for the reduced and denatured enzyme by gel electrophoresis in sodium dodecyl sulfate. The isoelectric point was 4.48. The enzyme was inhibited by p-chloromercuribenzoate. The presence of divalent cations (i.e., Co2+ or Zn2+) is essential for its activity. 相似文献
60.