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151.
152.
Nakamura H Kawasaki Y Arakawa N Saeki M Maeda S Koyama Y Baba A Matsuda T 《Neurochemical research》2000,25(3):385-387
The effects of the Na+-Ca2+ exchange inhibitor 2-[2-[4-(4-nitrobenzyloxy)phenyl]ethyl]isothiourea methanesulfonate (KB-R7943) on depolarization-induced Ca2+ signal and [3H]noradrenaline release were examined in SH-SY5Y cells. KB-R7943 at 10 M significantly inhibited high K+-induced increase in intracellular Ca2+ concentration. KB-R7943 also inhibited high K+-evoked release of [3H]noradrenaline from the cells. These findings suggest that the Na+-Ca2+ exchanger in the reverse mode is involved at least partly in depolarization-induced transmitter release. 相似文献
153.
Muto T Hotta Y Miyazeki K Ando H Ishikawa N Hasegawa T Sugimoto Y Yamada J Miki Y 《Molecular and cellular biochemistry》2005,272(1-2):119-132
The protective effects of sarpogrelate (SG), a 5-HT2A antagonist, were investigated in perfused guinea-pig Langendorff hearts subjected to ischemia and reperfusion. Changes in cellular levels of high phosphorous energy, NO and Ca2+ in the heart together with simultaneous recordings of left ventricular developed pressure (LVDP) were monitored using an nitric oxide (NO) electrode, fluorometry and 31P-NMR. The recovery of LVDP from ischemia by reperfusion was 30.1% in the control, while the treatment with SG (5×10-7 M) in pre- and post-ischemia hearts produced a gradual increase to 73.1 and 53.6%, respectively. At the final stage of ischemia, the intracellular concentration of Ca2+ ([Ca2+i) and release of NO increased with no twitching and remained at a high steady level. The addition of SG increased the transient NO signal (TNO) level at the end of ischemia compared with the control, but [Ca2+]i during ischemia decreased. Meanwhile, mitochondrial Ca2+ uptake on acidification or Ca2+ content changes of the perfusate was suppressed by pre-treatment with SG or the KATP channel opener diazoxide, but not the KATP channel blocker 5-HD. The myocardial NO elevated with 5-HT in normal Langendorff hearts was suppressed by the treatment with SG. Therefore, the existence of the 5HT2A receptor in a Langendorff heart was anticipated. By in vitro EPR, SG was found to directly quench the hydroxy radical. Thus, these findings suggested that the 5-HT2A receptor induced in ischemia–reperfusion plays an important role in the mitochondrial KATP channel of hearts in close relation with NO and active oxygen radicals. 相似文献
154.
Kiyofumi Murakami Takayuki Sano Naohisa Kure Katsushi Ishii Tatsuya Yasunaga 《Biopolymers》1983,22(9):2035-2044
The interaction of methyl orange with poly(L -lysine) was studied kinetically by the stopped-flow technique with CD detection, as well as by static CD titration experiments. In the static experiments, the differences observed in the polymer-to-dye ratio dependences of the CD spectra and absorption spectra suggested at least two kinds of bound states of the methyl orange attached to the polymer. The kinetic experiments using the stopped-flow apparatus, however, revealed four distinct reaction processes. The reaction mechanism was elucidated from the concentration dependence of the time constant for each process as follows: the first process was attributed to the bimolecular binding step of methyl orange to the side chain of poly(L -lysine), the second and third process were ascribed to the intramolecular reaction of the polymer–dye complex, and the fourth process was found to be the intermolecular aggregation of the polymer–dye complex. The origin of the stacking of methyl orange on poly(L -lysine) is discussed on the basis of the characteristics of signal amplitudes obtained from the kinetic experiments for these processes. 相似文献
155.
Yasuhito Ishigaki Yuka Nakamura Takanori Tatsuno Shaofu Ma Naohisa Tomosugi 《Experimental biology and medicine (Maywood, N.J.)》2015,240(4):438-445
The RNA-binding protein 8A (RBM8A)–mago-nashi homolog, proliferation-associated (Magoh) complex is a component of the exon junction complex (EJC) required for mRNA metabolism involving nonsense-mediated mRNA decay (NMD). RBM8A is a phosphorylated protein that plays some roles in NMD. However, the detailed status and mechanism of the phosphorylation of RBM8A is not completely understood. Therefore, in this study, we analyzed in detail RBM8A phosphorylation in human cells. Accordingly, analysis of the phosphorylation status of RBM8A protein in whole-cell lysates by using Phos-tag gels revealed that the majority of endogenous RBM8A was phosphorylated throughout the cell-cycle progression. Nuclear and cytoplasmic RBM8A and RBM8A in the EJC were also found to be mostly phosphorylated. We also screened the phosphorylated serine by mutational analysis using Phos-tag gels to reveal modifications of serine residues 166 and 168. A single substitution at position 168 that concomitantly abolished the phosphorylation of serine 166 suggested the priority of kinase reaction between these sites. Furthermore, analysis of the role of the binding protein Magoh in RBM8A phosphorylation revealed its inhibitory effect in vitro and in vivo. Thus, we conclude that almost all synthesized RBM8A proteins are rapidly phosphorylated in cells and that phosphorylation occurs before the complex formation with Magoh. 相似文献
156.
Nakashima Naohisa Kato Tomomichi Morozumi Tomoki Tsujimoto Katsuto Akitsu Tomoko Kawaguchi Nasahara Kenlo Nishida Murayama Shohei Muraoka Hiroyuki Noda Hibiki M. 《Journal of plant research》2021,134(4):713-728
Journal of Plant Research - Solar-induced chlorophyll fluorescence (SIF) emissions were estimated by the "area-ratio Fraunhofer line depth (aFLD) method", a new retrieval methodology in... 相似文献
157.
Two oligosaccharides were isolated from a partial hydrolysis of the lipopolysaccharide from Shigella dysenteriae which had a common antigen with human red blood cells (RBC). A disaccharide sh-2 had a structure of O-β-D-galactosyl-(l→2)-D-galactose, and was active in the hemagglutination inhibition test between human O RBC and anti-S. dysenteriae chicken serum. A tetrasaccharide sh-12 was composed of galactose and rhamnose and was a more effective inhibitor than sh-2. Both oligosaccharides were assumed to be derived from the determinant structure of a common antigen. Serological specificity of the hemagglutinin in chicken serum was examined using these oligosaccharides and human milk oligosaccharides, and the 1→2 linked galactosyl residue was supposed to be important for determination of the heterophile RBC antigenicity. 相似文献