Malignant lymphoma of the uterine cervix. Cytologic diagnosis of a case with immunocytochemical corroboration

The cytologic findings in a case of primary malignant lymphoma of the uterine cervix initially suggested the presence of a malignant neoplasm. By the use of immunologic methods, the lymphoma cells were shown to possess the nature of B lymphocytes. The need for cytochemical study to make a definitive diagnosis of this rare condition is emphasized. The cytologic findings are compared with those of cytologically similar conditions in the cervix: reticulum-cell sarcoma, chloroma and endometrial stromal sarcoma.     

Mating reaction in Saccharomyces cerevisiae VI. Effect of 2-deoxyglucose on conjugation

The effect of 2-deoxyglucose (2-dG) on the mating reaction ofSaccharomyces cerevisiae was investigated and the followingresults were obtained. 1) The cell fusion process of the mating reaction was completelyinhibited by 0.05% 2-dG added to a culture medium containing2% D-glucose. This inhibition was partially reversed by raisingthe glucose concentration in the medium. 2) Sexual cell agglutination was hardly affected by 2-dG. 3) 2-dG at concentrations inhibiting cell fusion considerablysuppressed the incorporation of ¹⁴C-glucose into the cell wallpolysaccharides, glucan and mannan. 4) Glucose uptake and protein synthesis were only slightly inhibitedby 2-dG. 5) No enhancement of bulk polysaccharide synthesis was detectedduring mating. ¹Present address: Biological Institute, Faculty of Science,Nagoya University, Chikusa-ku, Nagoya 464, Japan. (Received April 20, 1974; )     

Genetic variations ofCottus nozawae populations from five tributaries of the Shiribetsu River of southern Hokkaido,Japan

To estimate genetic differentiation and heterogeneity in the landlocked river sculpin,Cottus nozawae, between tributary populations in the same river-system, 107 specimens were captured from 5 tributaries of the Shiribetsu river (course length 128 km), Hokkaido Island and surveyed for allozyme variations and restriction fragment length polymorphisms of mitochondrial DNA (mtDNA). Three and two alleles were seen at theIdh-2 andPgm loci, respectively, but only one locus,Idh-2, out of twenty loci examined was regarded as polymorphic, since the frequency of the most common allele did not exceed 0.95. Three different mtDNA haplotypes were detected, there being replacement of them between the tributary populations. Heterogeneities of allele and haplotype frequencies were significant between some tributary populations, suggesting that genetic differentiation has occurred between them.     

Changes in sporulation ability during the vegetative cell cycle insaccharomyces cerevisiae

The relationship between the sporulation ability and the stage of the vegetative cell cycle was studied. The ability of mature cells to sporulate was the highest just before bud initiation and decreased rapidly as soon as detectable buds appeared. The mitotic DNA synthesis was competitive with the premeiotic one.