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131.
132.
Kamimura K Fujise M Villa F Izumi S Habuchi H Kimata K Nakato H 《The Journal of biological chemistry》2001,276(20):17014-17021
Heparan sulfate, one of the most abundant components of the cell surface and the extracellular matrix, is involved in a variety of biological processes such as growth factor signaling, cell adhesion, and enzymatic catalysis. The heparan sulfate chains have markedly heterogeneous structures in which distinct sequences of sulfate groups determine specific binding properties. Sulfation at each different position of heparan sulfate is catalyzed by distinct enzymes, sulfotransferases. In this study, we identified and characterized Drosophila heparan sulfate 6-O-sulfotransferase (dHS6ST). The deduced primary structure of dHS6ST exhibited several common features found in those of mammalian HS6STs. We confirmed that, when the protein encoded by the cDNA was expressed in COS-7 cells, it showed HS6ST activity. Whole mount in situ hybridization revealed highly specific expression of dHS6ST mRNA in embryonic tracheal cells. The spatial and temporal pattern of dHS6ST expression in these cells clearly resembles that of the Drosophila fibroblast growth factor (FGF) receptor, breathless (btl). RNA interference experiments demonstrated that reduced dHS6ST activity caused embryonic lethality and disruption of the primary branching of the tracheal system. These phenotypes were reminiscent of the defects observed in mutants of FGF signaling components. We also show that FGF-dependent mitogen-activated protein kinase activation is significantly reduced in dHS6ST double-stranded RNA-injected embryos. These findings indicate that dHS6ST is required for tracheal development in Drosophila and suggest the evolutionally conserved roles of 6-O-sulfated heparan sulfate in FGF signaling. 相似文献
133.
Nakagawa N 《Primates; journal of primatology》2003,44(1):3-11
Phytochemical or nutrient analyses of primate diets have revealed clues to their food selection in a single species. On the
other hand, few interspecific comparisons of phytochemical or nutrient composition of primate diets have been made, although
diets are considered to differ in phytochemical or nutrient content from primate species to species, since different species
have different body weights and different morphological and physiological characteristics. I compared the nutrient content
of diet between patas monkeys (Erythrocebus patas) and tantalus monkeys (Cercopithecus aethiops tantalus) living sympatrically in Cameroon. Patas subsisted on a smaller number of food items, most of which were also tantalus food
items. Then, I compared the protein–fiber ratio and the available energy content of the food items eaten by patas (patas foods)
with those items eaten only by tantalus (tantalus foods). Both variables were higher in patas than tantalus foods, although
there was no significant difference in available energy of plant foods. Next, when I performed discriminant analysis for patas
foods and tantalus foods, employing the above two variables, a discriminant function with positive coefficients for both variables
was obtained. The mean discriminant-function score of patas foods was higher than that of tantalus foods. Despite being somewhat
larger in weight, patas selectively fed on a smaller number of foods of higher quality than did tantalus. I discuss why the
results are inconsistent with a well known body weight–diet relationship (Jarman–Bell principle). Energy-efficient locomotion
enables patas to exploit not only small dispersed food items of high quality but also areas where high-quality foods are distributed
in clumps.
Electronic Publication 相似文献
134.
We constructed a sex allocation model for local mate competition considering the asymmetry of competitive abilities among sons. This model assumes two females of a parasitoid wasp oviposit on the same host in sequential order. The evolutionarily stable strategy will be in either Stackelberg or Nash equilibrium, depending on whether the females can recognize their opponent's sex ratio or not, respectively. The Nash equilibrium predicts the second female produce more males than the first. If the second female is able to know and respond to the strategy of the first (a Stackelberg equilibrium), the first will decide an optimal sex ratio assuming that the second reply to it. Under such an assumption, our model predicts that not producing sons is adaptive for the second female when the sons she produces have low competitive ability. Males of parasitoid wasps Melittobia spp. are engaged in lethal male-male combat, indicating large asymmetry in mating success among sons. If females have the ability to recognize their opponent's sex ratio, our model suggests that the severe lethal male-male combat may be one factor explaining their extremely female-biased sex ratio that is unexplainable by pre-existent models. 相似文献
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Oikawa T Kamimura Y Akiba H Yagita H Okumura K Takahashi H Zeniya M Tajiri H Azuma M 《Journal of immunology (Baltimore, Md. : 1950)》2006,177(7):4281-4287
Tim-3, a member of the T cell Ig mucin (TIM) family regulates effector Th1 responses. We examined Tim-3 and its ligand expression as well as the effects of anti-Tim-3 mAb treatment in a murine model of acute graft-vs-host disease (aGVHD). In mice with aGVHD, Tim-3 expression was markedly up-regulated on splenic and hepatic CD4+ and CD8+ T cells, dendritic cells (DCs), and macrophages, and this was especially dramatic in hepatic CD8+ T cells. Both donor- and host-derived CD8+ T cells induced similar levels of Tim-3. Tim-3 ligand expression was also up-regulated in splenic T cells, DCs, and macrophages, but not in the hepatic lymphocytes. The administration of anti-Tim-3 mAbs accelerated aGVHD, as demonstrated by body weight loss, reduction in total splenocyte number, and infiltration of lymphocytes in the liver. IFN-gamma expression by splenic and hepatic CD4+ and CD8+ T cells was significantly augmented by anti-Tim-3 mAb treatment. In addition, the cytotoxicity against host alloantigen by donor CD8+ T cells was enhanced. These results demonstrate that the anti-Tim-3 treatment in aGVHD augmented the activation of effector T cells expressing IFN-gamma or exerting cytotoxicity. Our results suggest that Tim-3 may play a crucial role in the regulation of CD8+ T cells responsible for the maintenance of hepatic homeostasis and tolerance. 相似文献
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