全文获取类型
收费全文 | 1553篇 |
免费 | 102篇 |
出版年
2023年 | 10篇 |
2022年 | 11篇 |
2021年 | 18篇 |
2020年 | 13篇 |
2019年 | 15篇 |
2018年 | 20篇 |
2017年 | 20篇 |
2016年 | 25篇 |
2015年 | 29篇 |
2014年 | 43篇 |
2013年 | 97篇 |
2012年 | 77篇 |
2011年 | 69篇 |
2010年 | 50篇 |
2009年 | 42篇 |
2008年 | 74篇 |
2007年 | 63篇 |
2006年 | 78篇 |
2005年 | 55篇 |
2004年 | 52篇 |
2003年 | 61篇 |
2002年 | 56篇 |
2001年 | 60篇 |
2000年 | 55篇 |
1999年 | 58篇 |
1998年 | 30篇 |
1997年 | 18篇 |
1996年 | 12篇 |
1995年 | 13篇 |
1994年 | 8篇 |
1993年 | 11篇 |
1992年 | 35篇 |
1991年 | 33篇 |
1990年 | 32篇 |
1989年 | 33篇 |
1988年 | 18篇 |
1987年 | 16篇 |
1986年 | 25篇 |
1985年 | 20篇 |
1984年 | 25篇 |
1983年 | 22篇 |
1982年 | 9篇 |
1981年 | 13篇 |
1980年 | 13篇 |
1979年 | 19篇 |
1978年 | 12篇 |
1974年 | 11篇 |
1972年 | 14篇 |
1968年 | 8篇 |
1967年 | 8篇 |
排序方式: 共有1655条查询结果,搜索用时 15 毫秒
101.
102.
In Mytilus mussels, paternal mitochondrial DNA (mtDNA) from sperm is known to be transmitted to offspring. This phenomenon is called doubly uniparental inheritance (DUI). Under DUI, sperm mtDNA (M type) is inherited only by males. Female mussels receive maternal mtDNA (F type). However, in our previous study, we showed female and unfertilized eggs have both F and M types. We hypothesized that the two M types both from sperm and unfertilized eggs were transmitted to offspring. To test the hypothesis, we examined the number of M type haplotypes in mature M. galloprovincialis. The M type in larvae was compared with those of the parents. Cross experiments were carried out to test the inheritance of M type. In six of 20 mature mussels, two M types were detected by sequence analysis and polymerase chain reaction-restriction fragment length polymorphism. In cross experiments of larval samples from five of 12 crosses, double peak wave was observed by single nucleotide polymorphisms analysis. In these larval samples, the higher peak wave was identical to the parental M type. Larvae received much more paternal M type than the maternal ones. We demonstrated that two M types from sperm and unfertilized eggs were transmitted to offspring in M. galloprovincialis. 相似文献
103.
104.
105.
Kouichi Kawamura Masashi Kubota Miki Furukawa Yasushi Harada 《Conservation Genetics》2007,8(5):1163-1176
The amago salmon, Oncorhynchus masou ishikawae, is an endemic subspecies of O. masou in Japan. Owing to the extensive stocking of hatchery fish throughout Japan, indigenous populations of O. m. ishikawae are now on the verge of extinction. We examined the genetic effects of stocking hatchery fish on wild populations in the
River Koza, Japan, using microsatellite and mitochondrial DNA (mtDNA) markers. For mtDNA, haplotype mt1, which is common in
wild populations, was present exclusively in isolated wild populations assumed to be unaffected by previous stocking, while
it was never observed in hatchery fish. Genetic diversity was much higher in wild populations in the stocked area, which shared
many mtDNA haplotypes with hatchery fish, than in isolated wild populations with haplotype mt1. Pairwise F
ST estimates based on microsatellites showed significant differentiation among the isolated populations with many microsatellite
loci monomorphic. Significant deviation from Hardy–Weinberg equilibrium was observed in wild populations in the area subject
to stocking, where a Bayesian-based assignment test showed a high level of introgression with hatchery fish. These results
suggest that wild populations with haplotype mt1, which became isolated through anthropogenic environmental change in the
1950–1960s, represent indigenous populations of O. m. ishikawae in the River Koza. They have low genetic diversity, most likely caused by genetic bottlenecks following damming and environmental
deterioration, while stocking of hatchery fish over the past 30 years apparently had a large impact on the genetic structure
of wild populations in the main channel of the River Koza. 相似文献
106.
Marko-Varga G Ogiwara A Nishimura T Kawamura T Fujii K Kawakami T Kyono Y Tu HK Anyoji H Kanazawa M Akimoto S Hirano T Tsuboi M Nishio K Hada S Jiang H Fukuoka M Nakata K Nishiwaki Y Kunito H Peers IS Harbron CG South MC Higenbottam T Nyberg F Kudoh S Kato H 《Journal of proteome research》2007,6(8):2925-2935
Personalized medicine allows the selection of treatments best suited to an individual patient and disease phenotype. To implement personalized medicine, effective tests predictive of response to treatment or susceptibility to adverse events are needed, and to develop a personalized medicine test, both high quality samples and reliable data are required. We review key features of state-of-the-art proteomic profiling and introduce further analytic developments to build a proteomic toolkit for use in personalized medicine approaches. The combination of novel analytical approaches in proteomic data generation, alignment and comparison permit translation of identified biomarkers into practical assays. We further propose an expanded statistical analysis to understand the sources of variability between individuals in terms of both protein expression and clinical variables and utilize this understanding in a predictive test. 相似文献
107.
Ching Fui Fui Joshua Mercylla Sara Kawamura Gunzo Senoo Shigeharu Lim Leong-Seng 《Ichthyological Research》2022,69(1):90-96
Ichthyological Research - The giant mottled eel Anguilla marmorata Quoy & Gaimard is an important aquaculture candidate in eel farming industry. The high economic value of the species leads... 相似文献
108.
109.
110.
Umeda Y Kako Y Mizutani K Iikura Y Kawamura M Seishima M Hayashi H 《Journal of lipid research》2001,42(8):1214-1219
This study was designed to determine whether gemfibrozil inhibits intestinal lipid absorption. Male Sprague-Dawley rats received an oral dose of 30 mg gemfibrozil/kg body weight for 14 days. Mesenteric lymph cannulation was performed, and a lipid infusion containing 40 micromol/h (35.4 mg/h) of radiolabeled triolein and 2.74 micromol/h (1.06 mg/h) of radiolabeled cholesterol with the addition of 1 mg/h of gemfibrozil was infused intraduodenally at a rate of 3 ml/h for 8 h. The lymph was collected, and the radioactivity levels of the lumen and gut mucosa were measured after the infusion. Lymph cholesterol transport was depressed in gemfibrozil-treated rats, in terms of mass measurements as well as radioactivity in a lesser degree. More radioactive cholesterol remained in the proximal portion of the intestinal lumen and mucosa in the treated rats than in the control rats. More radioactive triglycerides also remained in the proximal intestinal lumen of treated rats, although no difference in lymphatic triglyceride transport was observed between the groups. A significant portion of the radioactive cholesterol remained in the lumen in the gemfibrozil-treated rats. Gemfibrozil increased biliary cholesterol excretion. Thus, this study shows that gemfibrozil inhibits cholesterol absorption in rat intestine. 相似文献