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51.
Martha Cornejo Deborah Nambi Christopher Walheim Matthew Somerville Jacquae Walker Lino Kim Lauren Ollison Graciel Diamante Saurabh Vyawahare Maria Elena de Bellard 《Neurochemical research》2010,35(10):1643-1651
The Schwann cells are the myelinating glia of the peripheral nervous system that originated during development from the highly
motile neural crest. However, we do not know what the guidance signals for the Schwann cell precursors are. Therefore, we
set to test some of the known neurotrophins that are expressed early in developing embryos and have been shown to be critical
for the survival and patterning of developing glia and neurons. The goal of this study was to determine more specifically
if GDNF, NRG1 and NGF are chemoattractants and/or chemokinetic molecules for a Schwann cell precursor line, the Spl201. We
performed live chemoattraction assays, with imaging and also presented these molecules as part of their growing substrate.
Our results show for the first time that GDNF and NRG1 are potent chemoattractive and chemokinetic molecules for these cells
while NGF is a chemokinetic molecule stimulating their motility. 相似文献
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Polyhydroxyalkanoates (PHAs) are biodegradable polymers produced by microbes to overcome environmental stress. Commercial
production of PHAs is limited by the high cost of production compared to conventional plastics. Another hindrance is the brittle
nature and low strength of polyhydroxybutyrate (PHB), the most widely studied PHA. The needs are to produce PHAs, which have
better elastomeric properties suitable for biomedical applications, preferably from inexpensive renewable sources to reduce
cost. Certain unique properties of Bacillus subtilis such as lack of the toxic lipo-polysaccharides, expression of self-lysing genes on completion of PHA biosynthetic process
– for easy and timely recovery, usage of biowastes as feed enable it to compete as potential candidate for commercial production
of PHA. 相似文献
54.
Sheran HW Law Rudolf SS Wu Patrick KS Ng Richard MK Yu Richard YC Kong 《BMC molecular biology》2006,7(1):15-13
Background
Hypoxia-inducible factors (HIFs) are involved in adaptive and survival responses to hypoxic stress in mammals. In fish, very little is known about the functions of HIFs. 相似文献55.
Subhalaxmi Nambi Nirmalya Basu Sandhya S. Visweswariah 《The Journal of biological chemistry》2010,285(32):24313-24323
Cyclic AMP synthesized by Mycobacterium tuberculosis has been shown to play a role in pathogenesis. However, the high levels of intracellular cAMP found in both pathogenic and non-pathogenic mycobacteria suggest that additional and important biological processes are regulated by cAMP in these organisms. We describe here the biochemical characterization of novel cAMP-binding proteins in M. smegmatis and M. tuberculosis (MSMEG_5458 and Rv0998, respectively) that contain a cyclic nucleotide binding domain fused to a domain that shows similarity to the GNAT family of acetyltransferases. We detect protein lysine acetylation in mycobacteria and identify a universal stress protein (USP) as a substrate of MSMEG_5458. Acetylation of a lysine residue in USP is regulated by cAMP, and using a strain deleted for MSMEG_5458, we show that USP is indeed an in vivo substrate for MSMEG_5458. The Rv0998 protein shows a strict cAMP-dependent acetylation of USP, despite a lower affinity for cAMP than MSMEG_5458. Thus, this report not only represents the first demonstration of protein lysine acetylation in mycobacteria but also describes a unique functional interplay between a cyclic nucleotide binding domain and a protein acetyltransferase. 相似文献
56.
Reema K. Thalji Nambi Aiyar Elizabeth A. Davenport Joseph A. Erhardt Lorena A. Kallal Dwight M. Morrow Shobha Senadhi Cynthia L. Burns-Kurtis Joseph P. Marino 《Bioorganic & medicinal chemistry letters》2010,20(14):4104-4107
Benzofuran-substituted urea analogs have been identified as novel P2Y1 receptor antagonists. Structure–activity relationship studies around the urea and the benzofuran moieties resulted in compounds having improved potency. Several analogs were shown to inhibit ADP-mediated platelet activation. 相似文献
57.
S. Abdul Majeed K. S. N. Nambi G. Taju N. Sundar Raj N. Madan A. S. Sahul Hameed 《Cell biology and toxicology》2013,29(1):59-73
Rohu gill cell line (LRG) was established from gill tissue of Indian major carp (Labeo rohita), a freshwater fish cultivated in India. The cell line was maintained in Leibovitz's L-15 supplemented with 10 % foetal bovine serum (FBS). This cell line has been sub-cultured more than 85 passages over a period of 2 years. The LRG cell line consists of both epithelial and fibroblastic-like cells. The cells were able to grow at a wide range of temperatures from 22 to 32 °C, the optimum temperature being 28 °C. The growth rate of gill cells increased as the FBS proportion increased from 2 to 20 % at 28 °C. The plating efficiency was also high (34.37 %). The viability of the LRG cell line was 70–80 % after 6 months of storage in liquid nitrogen. The karyotype analysis revealed a diploid count of 50 chromosomes. The gill cells of rohu were successfully transfected with pEGFP-N1. Amplification of mitochondrial Cox1 gene using primers specific to L. rohita confirmed the origin of this cell line from L. rohita. The cytotoxicity of malathion was assessed in LRG cell line using multiple endpoints such as 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Neutral Red assay, Alamar Blue assay and Coomassie Blue protein assay. Acute toxicity assay on fish was conducted by exposing L. rohita for 96 h to malathion under static conditions. Statistical analysis revealed good correlation with r 2?=?0.946–0.990 for all combinations between endpoints employed. Linear correlations between each in vitro effective concentration 50 and the in vivo lethal concentration 50 data were highly significant. 相似文献
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We present an efficient computational architecture designed using supervised machine learning model to predict amyloid fibril
forming protein segments, named AmylPepPred. The proposed prediction model is based on bio-physio-chemical properties of
primary sequences and auto-correlation function of their amino acid indices. AmylPepPred provides a user friendly web interface
for the researchers to easily observe the fibril forming and non-fibril forming hexmers in a given protein sequence. We expect that
this stratagem will be highly encouraging in discovering fibril forming regions in proteins thereby benefit in finding therapeutic
agents that specifically aim these sequences for the inhibition and cure of amyloid illnesses.