排序方式: 共有71条查询结果,搜索用时 0 毫秒
61.
62.
63.
64.
65.
Carolin A. Müller Michelle Hawkins Renata Retkute Sunir Malla Ray Wilson Martin J. Blythe Ryuichiro Nakato Makiko Komata Katsuhiko Shirahige Alessandro P.S. de Moura Conrad A. Nieduszynski 《Nucleic acids research》2014,42(1):e3
Eukaryotic genomes are replicated from multiple DNA replication origins. We present complementary deep sequencing approaches to measure origin location and activity in Saccharomyces cerevisiae. Measuring the increase in DNA copy number during a synchronous S-phase allowed the precise determination of genome replication. To map origin locations, replication forks were stalled close to their initiation sites; therefore, copy number enrichment was limited to origins. Replication timing profiles were generated from asynchronous cultures using fluorescence-activated cell sorting. Applying this technique we show that the replication profiles of haploid and diploid cells are indistinguishable, indicating that both cell types use the same cohort of origins with the same activities. Finally, increasing sequencing depth allowed the direct measure of replication dynamics from an exponentially growing culture. This is the first time this approach, called marker frequency analysis, has been successfully applied to a eukaryote. These data provide a high-resolution resource and methodological framework for studying genome biology. 相似文献
66.
Epithelial–stromal interaction via Notch signaling is essential for the full maturation of gut‐associated lymphoid tissues 下载免费PDF全文
67.
Journal of Plant Research - Phegopteris decursivepinnata includes diploids, tetraploids, and triploid hybrids based on x = 30. We obtained polyploid progeny from triploid hybrids... 相似文献
68.
Sawada H Nakato H Togawa T Nakagoshi M Takikawa S Dohke K Iino T Mase K Yamamoto T Izumi S 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2003,134(3):519-527
We have cloned the full length of a novel cDNA named Bombyx mori cuticle protein that contains an AlaAlaProAla/Val-repeat (BMCPA) from a cDNA library of integument in the larval silkworm. Both a typical tandem repeat (A-A-P-A/V) for cuticle protein and a unique tandem repeat with Ser, Ala, Gly, Pro, Val, Tyr and Thr were observed in the predicted amino acid sequence of the cDNA encoding BMCPA. Approximately 80% of the amino acids in BMCPA were composed of Ser, Ala, Gly, Pro, Val and Tyr. Northern-hybridization analysis indicated that BMCPA mRNA is expressed only in the larval epidermis and that the expression pattern of the BMCPA gene in the developmental stage was observed mainly at the larval stage. We propose BMCPA may be a novel component of cuticle, and may play an important role in the integument of the larval silkworm. 相似文献
69.
Yamauchi S Nakato T Tsuchiya M Akiyama K Maruyama M Sugahara T Kishida T 《Bioscience, biotechnology, and biochemistry》2007,71(9):2248-2255
The benzyl mesylate was employed to construct the tetrasubstituted tetrahydrofuran lignan with avoiding Friedel-Crafts type of reaction. The optically pure 7,8-trans, 7',8'-trans, 7,7'-cis, and 8,8'-cis-virgatusin stereoisomers were synthesized. The enantiomeric excess was >99%. 相似文献
70.