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71.
Vinay Nair Monalisa Dutta Sowmya S Manian Ramya Kumari S Valadi K Jayaraman 《Bioinformation》2013,9(9):481-484
Penicillin-Binding Proteins are peptidases that play an important role in cell-wall biogenesis in bacteria and thus maintaining
bacterial infections. A wide class of β-lactam drugs are known to act on these proteins and inhibit bacterial infections by disrupting
the cell-wall biogenesis pathway. Penicillin-Binding proteins have recently gained importance with the increase in the number of
multi-drug resistant bacteria. In this work, we have collected a dataset of over 700 Penicillin-Binding and non-Penicillin Binding
Proteins and extracted various sequence-related features. We then created models to classify the proteins into Penicillin-Binding
and non-binding using supervised machine learning algorithms such as Support Vector Machines and Random Forest. We obtain a
good classification performance for both the models using both the methods. 相似文献
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Melanocyte–keratinocyte interaction induces calcium signalling and melanin transfer to keratinocytes
Preeti G. Joshi Nirmala Nair Gulnaz Begum Nanda B. Joshi Vilas P. Sinkar Shilpa Vora 《Pigment cell & melanoma research》2007,20(5):380-384
Physical contact between melanocytes and keratinocytes is a prerequisite for melanosome transfer to occur, but cellular signals induced during or after contact are not fully understood. Herein, it is shown that interactions between melanocyte and keratinocyte plasma membranes induced a transient intracellular calcium signal in keratinocytes that was required for pigment transfer. This intracellular calcium signal occurred due to release of calcium from intracellular stores. Pigment transfer observed in melanocyte–keratinocyte co‐cultures was inhibited when intracellular calcium in keratinocytes was chelated. We propose that a ‘ligand‐receptor’ type interaction exists between melanocytes and keratinocytes that triggers intracellular calcium signalling in keratinocytes and mediates melanin transfer. 相似文献
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Pereira LA Báo SN Barbosa MS da Silva JL Felipe MS de Santana JM Mendes-Giannini MJ de Almeida Soares CM 《FEMS yeast research》2007,7(8):1381-1388
Paracoccidioides brasiliensis is an important fungal pathogen. The disease it causes, paracoccidioidomycosis (PCM), ranges from localized pulmonary infection to systemic processes that endanger the life of the patient. Paracoccidioides brasiliensis adhesion to host tissues contributes to its virulence, but we know relatively little about molecules and the molecular mechanisms governing fungal adhesion to mammalian cells. Triosephosphate isomerase (TPI: EC 5.3.1.1) of P. brasiliensis (PbTPI) is a fungal antigen characterized by microsequencing of peptides. The protein, which is predominantly expressed in the yeast parasitic phase, localizes at the cell wall and in the cytoplasmic compartment. TPI and the respective polyclonal antibody produced against this protein inhibited the interaction of P. brasiliensis to in vitro cultured epithelial cells. TPI binds preferentially to laminin, as determined by peptide inhibition assays. Collectively, these results suggest that TPI is required for interactions between P. brasiliensis and extracellular matrix molecules such as laminin and that this interaction may play an important role in the fungal adherence and invasion of host cells. 相似文献
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Glyphidrilus annandalei Michaelson exhibits a pronounced breeding period extending from September to November. Breeding individuals achieve their maximum frequency in September, when 50 per cent of the whole population was with fully developed clitellum. Studies on the physical and chemical conditions of the habitat revealed that breeding in this worm depends the monsoon of the area creating optimum conditions for the life and activities of the organism. 相似文献
80.
Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139 总被引:7,自引:0,他引:7