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51.
Identification and characterization of two novel C‐type lectins from the larvae of housefly,Musca domestica L.
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Jing Zhou Nai‐ Nai Fang Ya Zheng Kai‐ Yu Liu Bin Mao Li‐ Na Kong Ya Chen Hui Ai 《Archives of insect biochemistry and physiology》2018,98(3)
Lectins and antimicrobial peptides (AMPs) are widely distributed in various insects and play crucial roles in primary host defense against pathogenic microorganisms. Two AMPs (cecropin and attacin) have been identified and characterized in the larvae of housefly. In this study, two novel C‐type lectins (CTLs) were obtained from Musca domestica, while their agglutinating and antiviral properties were evaluated. Real‐time PCR analysis showed that the mRNA levels of four immune genes (MdCTL1, MdCTL2, Cecropin, and Attacin) from M. domestica were significantly upregulated after injection with killed Gram‐negative Escherichia coli. Moreover, purified MdCTL1‐2 proteins can agglutinate E. coli and Staphylococcus aureus in the presence of calcium ions, suggesting their immune function is Ca2+ dependent. Sequence analysis indicated that typical WND and QPD motifs were found in the Ca2+‐binding site 2 of carbohydrate recognition domain from MdCTL1‐2, which was consistent with their agglutinating activities. Subsequently, antiviral experiments indicated that MdCTL1‐2 proteins could significantly reduce the infection rate of Spodoptera frugiperda 9 cells by the baculovirus Autographa californica multicapsid nucleopolyhedrovirus, indicating they might play important roles in insect innate immunity against microbial pathogens. In addition, MdCTL1‐2 proteins could effectively inhibit the replication of influenza H1N1 virus, which was similar to the effect of ribavirin. These results suggested that two novel CTLs could be considered a promising drug candidate for the treatment of influenza. Moreover, it is believed that the discovery of the CTLs with antiviral effects in M. domestica will improve our understanding of the molecular mechanism of insect immune response against viruses. 相似文献
52.
Helicobacter pylori Activates IL‐6‐STAT3 Signaling in Human Gastric Cancer Cells: Potential Roles for Reactive Oxygen Species
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观察了脓毒血症大鼠心肌II型PLA2 活性、蛋白质含量及其m RNA 的变化。结果发现, 脓毒血症早期与晚期心肌II型PLA2 活性较对照组分别降低25 .0 % (P < 0 .05)及增高47.6 % (P < 0 .01),II型PLA2 蛋白质含量分别降低27.0% 及增高48 .0 %( 均P < 0 .01); 心肌II型PLA2 m RNA合成率与含量呈现类似的双相变化, 在脓毒血症早、晚期mRNA 合成率分别降低45.0% 和升高70.0 % (均P < 0 .01),mRNA含量分别降低34.1 % 和增加157 .0% (均P< 0 .01) 。脓毒血症早、晚期心脏II型PLA2 m RNA半衰期无显著变化(P > 0.05) 。实验结果表明大鼠脓毒血症发生过程中心肌II型PLA2 活性呈现出先下降后升高的变化, 这一变化受其mRNA 转录水平的调节。 相似文献
56.
Jie Tian Yingxin Shi Shanshan Nai Qizhi Geng Leiliang Zhang Gong-Hong Wei Xingzhi Xu Jing Li 《遗传学报》2017,44(11):549-552
<正>Cytokinesis is the final stage of cell division that generates two daughter cells(Fededa and Gerlich,2012).The textbook version di-vides the plant and animal cell cytokinesis into two categories.Plant cells form a mid-zone phragmoplast via vesicle delivering and fusion,and cell wall materials are thus deposited.Animal cells form actomyosin contractile rings,which are the sole force that drives abscission.However,recent evidence has been mounting and pinpointing a pivotal role of membrane transport and subse- 相似文献
57.
Zeng QY Chen R Darmawan J Xiao ZY Chen SB Wigley R Le Chen S Zhang NZ 《Arthritis research & therapy》2008,10(1):R17-11
Introduction
Epidemiological studies of rheumatic diseases have been conducted during the past 20 years in China. The aim of this study was to clarify prevalence rates of common rheumatic diseases in China.Methods
Relevant reports of population-based surveys conducted from 1980 to 2006 were retrieved. Studies using the World Health Organization-International League of Associations for Rheumatology COPCORD (Community Oriented Program for Control of Rheumatic Diseases) protocol and those that did not employ this protocol but were published in recognized journals were identified and analyzed.Results
Thirty-eight surveys including 241,169 adults from 25 provinces/cities were pooled for analysis. The prevalence of rheumatic complaints ranged from 11.6% to 46.4%, varying by locality, study protocol and age of the people surveyed. Prevalence of symptomatic osteoarthritis (OA) varied from 5.1% to 20.8%, with common sites of involvement being the lumbar spine, knee joint and cervical spine. Compared with rates of radiographic and symptomatic knee OA in the USA, elderly men in Beijing exhibited similar prevalence rates and elderly women exhibited a higher prevalence. The prevalence of hip OA and hand OA was much lower in Chinese than in Caucasian populations, but both kinds of OA were more common in coal miners. The prevalence of ankylosing spondylitis ranged from 0.2% to 0.54% among Han ethnic Chinese and were lower among mixed ethnic populations. The prevalence of psoriatic arthritis ranged from 0.01% to 0.1%, and that of reactive arthritis was 0.02%; undifferentiated spondyloarthropathy was identified in 0.64% to 1.2% of the individuals included in the surveys. The prevalence of rheumatoid arthritis (RA) ranged from 0.2% to 0.93%, with the highest rate being reported from a Taiwan urban area. In mainland China there were no significant differences in prevalence of RA between the northern and southern parts of China, or between different ethnic groups. The prevalence of hyperuricemia increased after the 1980s. The prevalence of gout was found to have increased in recent decades from 0.15% to 1.98%, apart from in the Taiwan aborigines, among whom the highest prevalence rate of 11.7% was recorded. The prevalence of primary Sjögren's syndrome in Beijing was 0.77% by the Copenhagen criteria and 0.33% by the San Diego criteria. The prevalence of soft tissue rheumatism was 2.5% to 5.7%. Fibromyalgia was seldom observed in China.Conclusion
Rheumatic diseases are common in China. The prevalence of rheumatic complaints varied with the locality surveyed. The prevalence of OA is comparable with that in Western countries but varies in terms of joint involvement. The prevalence of ankylosing spondylitis is similar to that in Caucasians. Except in Taiwan, the prevalence of RA in China is lower than that in developed countries. The prevalence of hyperuricemia and gout increased after the 1980s, but it remains lower than that in developed countries. More studies are required to evaluate prevalence rates among minority groups in the west and northwest parts of China, and further study is needed to address fibromyalgia in China. 相似文献58.
Sein Lwin Yasuo Inoshima Hiroshi Ueno Naotaka Ishiguro 《Cell and tissue research》2009,337(1):125-135
To investigate the uptake and transport patterns of variously sized particles in Peyer’s patches (PPs) of calves, intestinal
loops were created in four newborn and two 2-month-old calves, and the loops were inoculated with various particles, including
carbon black, fluorescein isothiocyanate (FITC)-labeled latex, FITC-labeled dextran, bovine serum, and recombinant mouse prion
protein (rMPrP). The intestinal loops were recovered at 3, 6, 9, and 24 h in newborn calves and at 24 h in 2-month-old calves
after inoculation, and the transport of the particles was examined by histological and immunohistochemical means. The uptake
of the particles was quantified by estimation of signal intensities. A greater intensity was found in newborn calves compared
with the 2-month-old calves. The peak uptake of carbon black, FITC-labeled latex, and rMPrP in the PPs of the distal ileum
occurred at 6 h after inoculation in newborn calves and then progressively decreased with time. Uptake was also dependent
on the site within the small intestine and the size of the particle studied. The transport of carbon black, FITC-labeled latex,
and FITC-labeled dextran occurred via the bloodstream, the mesenteric lymph nodes, and the liver of newborn calves. rMPrP
was found primarily in the interfollicular regions of the submucosa of the distal ileum of newborn calves. Thus, distal ileal
PPs are probably more effective at particle absorption than the jejunal PPs, and the peak uptake of the PPs within the newborn
calf occurs at 6 h after inoculation.
This study was partly supported by a grant for BSE research from the Ministry of Health, Labour, and Welfare, Japan (17270701)
and Grant-in-Aid (no. 17380180) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan. 相似文献
59.
Ho‐Kee Koon Pui‐Shan Chan Ricky Ngok‐Shun Wong Zhen‐Guo Wu Maria Li Lung Chi‐Kwong Chang Nai‐Ki Mak 《Journal of cellular biochemistry》2009,108(6):1356-1363
Epidermal growth factor receptor (EGFR), a receptor often expressed in nasopharyngeal carcinoma (NPC) cells, is one of the recently identified molecular targets in cancer treatment. In the present study, the effects of combined treatment of Zn‐BC‐AM PDT with an EGFR inhibitor AG1478 were investigated. Well‐differentiated NPC HK‐1 cells were subjected to PDT with 1 µM of Zn‐BC‐AM and were irradiated at a light dose of 1 J/cm2 in the presence or absence of EGFR inhibitor AG1478. Specific protein kinase inhibitors of downstream EGFR targets were also used in the investigation. EGFR, Akt, and ERK were found constitutively activated in HK‐1 cells and the activities could be inhibited by the EGFR inhibitor AG1478. A sub‐lethal concentration of AG1478 was found to further enhance the irreversible cell damage induced by Zn‐BC‐AM PDT in HK‐1 cells. Pre‐incubation of the cells with specific inhibitors of EGFR (AG1478), PI3k/Akt (LY294002), or MEK/ERK (PD98059) before light irradiation were found to enhance Zn‐BC‐AM PDT‐induced formation of apoptotic cells. The efficacy of Zn‐BC‐AM PDT can be increased through the inhibition of EGFR/PI3K/Akt and EGFR/MEK/ERK signaling pathways in NPC cells. Combination therapy with Zn‐BC‐AM PDT and EGFR inhibitors may further be developed for the treatment of advanced NPC. J. Cell. Biochem. 108: 1356–1363, 2009. © 2009 Wiley‐Liss, Inc. 相似文献
60.
Yu-Shin Nai Tai-Chuan Wang Yun-Ru Chen Chu-Fang Lo 《Journal of invertebrate pathology》2009,102(2):110-119
A new multiple nucleopolyhedrovirus strain was isolated from casuarina moth, Lymantria xylina Swinhoe, (Lepidoptera: Lymantriidae) in Taiwan. This Lymantria-derived virus can be propagated in IPLB-LD-652Y and NTU-LY cell lines and showed a few polyhedra (occlusion bodies) CPE in the infected cells. The restriction fragment length polymorphism (RFLP) profiles of whole genome indicated that this virus is distinct from LyxyMNPV and the virus genome size was approximately 139 kbps, which was smaller than that of LyxyMNPV. The molecular phylogenetic analyses of three important genes (polyhedrin, lef-8 and lef-9) were performed. Polyhedrin, LEF-8 and LEF-9 putative amino acid analyses of this virus revealed that this virus belongs to Group II NPV and closely related to LdMNPV than to LyxyMNPV. The phylogenetic distance analysis was further clarified the relationship to LdMNPV and this virus provisionally named LdMNPV-like virus. A significant deletion of a 44 bp sequence found in LdMNPV-like virus was noted in the fp25k sequences of LdMNPV and LyxyMNPV and may play an important role in the few polyhedra CPE. In ultrastructural observations, the nuclei of the infected LD host cells contained large occlusion bodies (OBs), and few OBs, which presented as one or two OBs in a nucleus that was otherwise filled with free nuclocapsids and virions. We concluded that this LdMNPV-like virus is a new LdMNPV strain from L. xylina. 相似文献