全文获取类型
收费全文 | 239篇 |
免费 | 18篇 |
出版年
2022年 | 3篇 |
2021年 | 2篇 |
2020年 | 3篇 |
2019年 | 10篇 |
2018年 | 4篇 |
2017年 | 4篇 |
2016年 | 11篇 |
2015年 | 9篇 |
2014年 | 8篇 |
2013年 | 11篇 |
2012年 | 16篇 |
2011年 | 10篇 |
2010年 | 12篇 |
2009年 | 9篇 |
2008年 | 15篇 |
2007年 | 20篇 |
2006年 | 15篇 |
2005年 | 15篇 |
2004年 | 13篇 |
2003年 | 6篇 |
2002年 | 10篇 |
2001年 | 4篇 |
2000年 | 3篇 |
1999年 | 8篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 4篇 |
1991年 | 4篇 |
1990年 | 8篇 |
1989年 | 3篇 |
1986年 | 2篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1981年 | 1篇 |
排序方式: 共有257条查询结果,搜索用时 15 毫秒
21.
Rong S Ikeda H Sato Y Hirohashi Y Takamura Y Sahara H Sato T Maeda A Ohguro H Sato N 《Cancer immunology, immunotherapy : CII》2002,51(5):282-290
Recoverin is a photoreceptor-specific calcium binding protein that is only expressed in the retina in normal tissues. Aberrant expression of recoverin, however, has been observed in several cancer tissues and may cause a very rare autoimmune disease, cancer-associated retinopathy (CAR). It has been suggested that CAR-positive cancer patients have a more favorable prognosis than CAR-negative cancer patients. To estimate the status of recoverin-specific T cells in such cancer patients, we generated an HLA-A24-recoverin peptide tetramer. By use of the tetramer, we could directly assess the frequency of CTL precursors (CTLp) of 20 HLA-A24(+) cancer patients with ten colon, six stomach and four breast cancers, and seven healthy individuals. Four cancer patients showed a CTLp frequency higher than 0.9%. Seven cancer patients including the former four patients and two healthy individuals showed specific anti-recoverin cytotoxic responses in an HLA-A24-restricted manner after in vitro stimulation with the recoverin peptide. Moreover, five cancer patients analyzed in an independent experiment using different peripheral blood mononuclear cells (PBMC) samples showed similar CTLp and cytotoxic T lymphocyte (CTL) frequencies and cytotoxic responses, suggesting that the CTLp frequency analyzed by the tetramer and the cytotoxic response may have a good correlation. Thus, we hypothesize that anti-recoverin CTLp may exist in some cancer patients, and that anti-recoverin CTL may be readily induced. 相似文献
22.
Sahara K Takemura Y 《Journal of experimental zoology. Part A, Comparative experimental biology》2003,297(2):196-200
The silkworm, Bombyx mori, has a dimorphic sperm system. The eupyrene sperm is the sperm to fertilize eggs and the apyrene sperm plays a crucial role for assisting fertilization. Heat-treated (33 degrees C for 96h) Daizo (DH) males, one of the strains in the silkworm, produce only eupyrene sperm, while in triploid males only apyrene sperm are functional. Though both types of males are found to be sterile, double copulation of the two males with a single female greatly increases fertility. Here we examined the fertilizing ability of eupyrene and apyrene sperm by means of an artificial insemination technique previously established in B. mori. Neither the eupyrene sperm collected from DH males, nor the apyrene sperm from triploid males have the ability to fertilize eggs. Artificial insemination with the mixture of eupyrene and apyrene sperm leveled up the frequency of fertilized eggs to more than 80%. When cryopreserved DH sperm (eupyrene sperm) were subjected to the same experiment, more than 95% fertilized eggs were obtained. These results confirmed that apyrene sperm play an important and indispensable role in fertilization in B. mori. Separate collection of functional eupyrene sperm and functional apyrene sperm and success of fertilization by means of the artificial insemination technique are applicable for further studies to elucidate the function of apyrene sperm. 相似文献
23.
Kanki K Torigoe T Hirai I Sahara H Kamiguchi K Tamura Y Yagihashi A Sato N 《Microbiology and immunology》2000,44(12):1051-1061
The nature of target molecules of natural killer (NK) cell-mediated lysis remains to be elucidated. As we previously reported, mAb 109 recognizes one of the tumor-associated antigens, designated as 109 antigen (Ag), expressed on the cell surface of rat fibrosarcomas W31 and W14, which are transformants of WFB (rat fetal fibroblast cell line) with H-ras oncogene. 109Ag was thought to be a target structure of NK cells since mAb 109 inhibited NK cell-mediated lysis against W31 and W14. Here, we demonstrate by molecular cloning that 109Ag is identical to rat CD44. Immunoprecipitation and immunoblotting studies also showed that mAb 109 and anti-rat CD44 mAb OX-50 recognize the same protein of W31 cell lysates with an 86 kDa molecular size. CD44 was suggested to be a target structure of NK cell-mediated lysis; however, rat CD44 cDNA transfection alone into CD44 null cell lines did not result in up-regulation of target cell susceptibility to NK cell-mediated lysis. Our results therefore indicated that CD44 may play a crucial role as one of the target structures in our rat fibrosarcoma system though the cell surface expression of CD44 alone does not affect NK susceptibility of the target cells. 相似文献
24.
25.
AFM observation of single,functioning ionotropic glutamate receptors reconstituted in lipid bilayers
Nahoko Kasai Chandra S. Ramanujan Ichiro Fujimoto Akiyoshi Shimada John F. Ryan Keiichi Torimitsu 《Biochimica et Biophysica Acta (BBA)/General Subjects》2010
Background
Ionotropic glutamate receptors (iGluRs) are responsible for extracellular signaling in the central nervous system. However, the relationship between the overall structure of the protein and its function has yet to be resolved. Atomic force microscopy (AFM) is an important technique that allows nano-scale imaging in liquid. In the present work we have succeeded in imaging by AFM of the external features of the most common iGluR, AMPA-R (α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor), in a physiological environment.Methods
Homomeric GluR3 receptors were over-expressed in insect cells, purified and reconstituted into lipid membranes. AFM images were obtained in a buffer from membranes immobilized on a mica substrate.Results
Using Au nanoparticle-conjugated antibodies, we show that proteins reconstitute predominantly with the N-terminal domain uppermost on the membrane. A tetrameric receptor structure is clearly observed, but it displays considerable heterogeneity, and the dimensions differ considerably from cryo-electron microscopy measurements.Conclusions
Our results indicate that the extracellular domains of AMPA-R are highly flexible in a physiological environment.General significance
AFM allows us to observe the protein surface structure, suggesting the possibility of visualizing real time conformational changes of a functioning protein. This knowledge may be useful for neuroscience as well as in pharmaceutical applications. 相似文献26.
Nahoko Shikata Yukihiro Maki Masahiko Nakatsui Masato Mori Yasushi Noguchi Shintaro Yoshida Michio Takahashi Nobuo Kondo Masahiro Okamoto 《Amino acids》2010,38(1):179-187
The changes in the concentrations of plasma amino acids do not always follow the flow-based metabolic pathway network. We
have previously shown that there is a control-based network structure among plasma amino acids besides the metabolic pathway
map. Based on this network structure, in this study, we performed dynamic analysis using time-course data of the plasma samples
of rats fed single essential amino acid deficient diet. Using S-system model (conceptual mathematical model represented by
power-law formalism), we inferred the dynamic network structure which reproduces the actual time-courses within the error
allowance of 13.17%. By performing sensitivity analysis, three of the most dominant relations in this network were selected;
the control paths from leucine to valine, from methionine to threonine, and from leucine to isoleucine. This result is in
good agreement with the biological knowledge regarding branched-chain amino acids, and suggests the biological importance
of the effect from methionine to threonine. 相似文献
27.
Yusuke Hirasawa Tomokazu Shoji Takashi Arai Alfarius E. Nugroho Jun Deguchi Takahiro Hosoya Nahoko Uchiyama Yukihiro Goda Khalijah Awang A. Hamid A. Hadi Motoo Shiro Hiroshi Morita 《Bioorganic & medicinal chemistry letters》2010,20(6):2021-2024
A new bisindole alkaloid, bisleuconothine A (1) consisting of an eburnane–aspidosperma type skeleton, was isolated from the bark of Leuconotis griffithii. The structure including absolute stereochemistry was elucidated on the basis of 2D NMR data and X-ray analysis. Bisleuconothine A (1) showed cell growth inhibitory activity against various human cancer cell lines. 相似文献
28.
Mariko Kato Nahoko Nagasaki-Takeuchi Yuki Ide Rie Tomioka Masayoshi Maeshima 《Plant signaling & behavior》2010,5(7):848-850
In plants, Ca2+, phosphatidylinositol phosphates (PtdInsPs) and inositol phosphates are major components of intracellular signaling. Several kinds of proteins and enzymes, such as calmodulin (CaM), protein kinase, protein phosphatase, and the Ca2+ channel, mediate the signaling. Two new Ca2+-binding proteins were identified from Arabidopsis thaliana and named PCaP1 and PCaP2 [plasma membrane (PM)-associated Ca2+(cation)-binding protein 1 and 2]. PCaP1 has an intrinsically disordered region in the central and C-terminal parts. The PCaP1 gene is expressed in most tissues and the PCaP2 gene is expressed predominantly in root hairs and pollen tubes. We recently demonstrated that these proteins are N-myristoylated, stably anchored in the PM, and are bound with phosphatidylinositol phosphates, especially PtdInsP2s. Here we propose a model for the switching mechanism of Ca2+-signaling mediated by PtdInsPs. Ca2+ forms a complex with CaM (Ca2+-CaM) when there is an increase in the cytosol free Ca2+. The binding of PCaPs with Ca2+-CaM causes PCaPs to release PtdInsPs. Until the release of PtdInsPs, the signaling is kept in the resting state.Key words: calcium signal, calmodulin, inositol phosphate, intrinsically disordered protein, myristoylation, phosphatidylinositol phosphate, plasma membrane 相似文献
29.
Junichi Matsubara Masaya Ono Kazufumi Honda Ayako Negishi Hideki Ueno Takuji Okusaka Junji Furuse Koh Furuta Emiko Sugiyama Yoshiro Saito Nahoko Kaniwa Junichi Sawada Ayako Shoji Tomohiro Sakuma Tsutomu Chiba Nagahiro Saijo Setsuo Hirohashi Tesshi Yamada 《Molecular & cellular proteomics : MCP》2010,9(4):695-704
30.